scholarly journals Measurement of Free Ca in Sarcoplasmic Reticulum in Perfused Rabbit Heart Loaded with 1,2-Bis(2-amino-5,6-difluorophenoxy)ethane-N,N,N′,N′-tetraacetic Acid by F NMR

1996 ◽  
Vol 271 (13) ◽  
pp. 7398-7403 ◽  
Author(s):  
Weina Chen ◽  
Charles Steenbergen ◽  
Louis A. Levy ◽  
Joseph Vance ◽  
Robert E. London ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Rabbit Heart ◽  
Tetraacetic Acid ◽  
Perfused Rabbit Heart

scholarly journals Regulation of the Ca 2+ Gradient Across the Sarcoplasmic Reticulum in Perfused Rabbit Heart

1998 ◽  
Vol 83 (9) ◽  
pp. 898-907 ◽  
Author(s):  
Weina Chen ◽  
Robert London ◽  
Elizabeth Murphy ◽  
Charles Steenbergen
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Rabbit Heart ◽  
Perfused Rabbit Heart

High-resolution1H NMR imaging of regional ischemia in the isolated perfused rabbit heart at 4.7 T

1991 ◽  
Vol 21 (1) ◽  
pp. 144-150 ◽  
Author(s):  
John C. Chatham ◽  
Stacey Ackerman ◽  
Stephen J. Blackband
Keyword(s):  
Rabbit Heart ◽  
Nmr Imaging ◽  
Regional Ischemia ◽  
Perfused Rabbit Heart

Na+ entry via store-operated channels modulates Ca2+signaling in arterial myocytes

2000 ◽  
Vol 278 (1) ◽  
pp. C163-C173 ◽  
Author(s):  
Assaf Arnon ◽  
John M. Hamlyn ◽  
Mordecai P. Blaustein
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Cyclopiazonic Acid ◽  
General Mechanism ◽  
Tetraacetic Acid ◽  
Pump Activity ◽  
In Cells

In many nonexcitable cells, hormones and neurotransmitters activate Na+ influx and mobilize Ca2+ from intracellular stores. The stores are replenished by Ca2+influx via “store-operated” Ca2+ channels (SOC). The main routes of Na+ entry in these cells are unresolved, and no role for Na+ in signaling has been recognized. We demonstrate that the SOC are a major Na+ entry route in arterial myocytes. Unloading of the Ca2+stores with cyclopiazonic acid (a sarcoplasmic reticulum Ca2+ pump inhibitor) and caffeine induces a large external Na+-dependent rise in the cytosolic Na+ concentration. One component of this rise in cytosolic Na+ concentration is likely due to Na+/Ca2+exchange; it depends on elevation of cytosolic Ca2+ and is insensitive to 10 mM Mg2+ and 10 μM La3+. Another component is inhibited by Mg2+ and La3+, blockers of SOC; this component persists in cells preloaded with 1,2-bis(2-aminophenoxy)ethane- N, N, N′, N′-tetraacetic acid to buffer Ca2+ transients and prevent Na+/Ca2+exchange-mediated Na+ entry. This Na+ entry apparently is mediated by SOC. The Na+ entry influences Na+ pump activity and Na+/Ca2+exchange and has unexpectedly large effects on cell-wide Ca2+ signaling. The SOC pathway may be a general mechanism by which Na+ participates in signaling in many types of cells.

Stability of myocardial O2 consumption-pressure-volume area relation in red cell-perfused rabbit heart

1991 ◽  
Vol 261 (5) ◽  
pp. H1630-H1635
Author(s):  
H. Yaku ◽  
B. K. Slinker ◽  
E. S. Myhre ◽  
M. W. Watkins ◽  
M. M. Lewinter
Keyword(s):  
Diastolic Pressure ◽  
Mechanical Energy ◽  
Rabbit Heart ◽  
Cardiac Contraction ◽  
Red Cell ◽  
O2 Consumption ◽  
Perfused Rabbit Heart ◽  
Total Mechanical Energy ◽  
Heart Preparation ◽  
Myocardial O2 Consumption

We evaluated the mechanical and energetic stability of the isolated rabbit heart perfused with a suspension of bovine red cells in Krebs-Henseleit buffer in terms of the pressure-volume area (PVA) concept. PVA, the area surrounded by the end-systolic and end-diastolic pressure-volume (P-V) relations and the systolic P-V trajectory of the P-V diagram, represents the total mechanical energy generated by each cardiac contraction. Myocardial O2 consumption (VO2) per beat has been reported to be highly linearly correlated with PVA. We used the slope and VO2-axis intercept of the VO2-PVA relation as energetic parameters and the maximum P-V ratio (Emax) as a contractility index of the left ventricle (LV) and compared them every 30 min for 120 min. Emax, the slope, and VO2 intercept of the VO2-PVA relation did not change significantly over 120 min compared with their control values [7.3 +/- 2.9 mmHg.ml-1.100 g LV, (1.67 +/- 0.40) x 10(-5) ml O2.mmHg-1.ml-1, and (3.26 +/- 1.01) x 10(-2) ml O2.beat-1.100 g LV-1, respectively]. However, the goodness of the linear fit of the VO2-PVA relation decreased after 90 min (r = 0.94 control, 0.62 at 90 min, and 0.64 at 120 min). Therefore, we conclude that the isolated bovine red cell-perfused rabbit heart preparation is stable for mechanical and energetic studies for at least 60 min.

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