scholarly journals Identification and Characterization of the Sodium-binding Site of Activated Protein C

1999 ◽  
Vol 274 (8) ◽  
pp. 4970-4976 ◽  
Author(s):  
Xuhua He ◽  
Alireza R. Rezaie
Keyword(s):  
Binding Site ◽  
Protein C ◽  
Activated Protein C ◽  
Sodium Binding ◽  
Identification And Characterization

scholarly journals Characterization of a Factor Xa Binding Site on Factor Va near the Arg-506 Activated Protein C Cleavage Site

2007 ◽  
Vol 282 (30) ◽  
pp. 21848-21855 ◽  
Author(s):  
Andrew J. Gale ◽  
Subramanian Yegneswaran ◽  
Xiao Xu ◽  
Jean-Luc Pellequer ◽  
John H. Griffin
Keyword(s):  
Binding Site ◽  
Cleavage Site ◽  
Protein C ◽  
Activated Protein C ◽  
Factor Xa ◽  
Factor Va

Characterization of a cDNA for Rhesus Monkey Protein C Inhibitor – Evidence for N-Terminal Involvement in Heparin Stimulation

1995 ◽  
Vol 74 (04) ◽  
pp. 1079-1087 ◽  
Author(s):  
Klaus-P Radtke ◽  
José A Fernández ◽  
Bruno O Villoutreix ◽  
Judith S Greengard ◽  
John H Griffin
Keyword(s):  
Rhesus Monkey ◽  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Amino Acid Sequences ◽  
Heparin Binding ◽  
Reactive Center ◽  
Protein C Inhibitor ◽  
Polymerase Chain

SummarycDNAs for protein C inhibitor (PCI) were cloned from human and rhesus monkey 1 liver RNAs by reverse transcription and polymerase chain reaction (PCR) amplification. Sequencing showed that rhesus monkey and human PCI cDNAs were 93% identical. Predicted amino acid sequences differed at 26 of 387 residues. Pour of these differences (T352M, N359S, R362K, L3631) were in the reactive center loop that is important for inhibitory specificity, and two were in the N-terminal helix (M8T, E13K) that is implicated in glycosaminoglycan binding. PCI in human or rhesus monkey plasma showed comparable inhibitory activity towards human activated protein C in the presence of 10 U/ml heparin. However, maximal acceleration of the inhibition of activated protein C required 5-fold lower heparin concentration for rhesus monkey than for human plasma, consistent with the interpretation that the additional positive charge (E13K) in a putative-heparin binding region increased the affinity for heparin.

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