scholarly journals Structural Characterization of Viral Ortholog of Human DNA Glycosylase NEIL1 Bound to Thymine Glycol or 5-Hydroxyuracil-containing DNA

2011 ◽  
Vol 287 (6) ◽  
pp. 4288-4298 ◽  
Author(s):  
Kayo Imamura ◽  
April Averill ◽  
Susan S. Wallace ◽  
Sylvie Doublié
Keyword(s):  
Structural Characterization ◽  
Dna Glycosylase ◽  
Thymine Glycol ◽  
Human Dna

scholarly journals Identification and Characterization of a Novel Human DNA Glycosylase for Repair of Cytosine-derived Lesions

2002 ◽  
Vol 277 (34) ◽  
pp. 30417-30420 ◽  
Author(s):  
Tapas K. Hazra ◽  
Yoke W. Kow ◽  
Zafar Hatahet ◽  
Barry Imhoff ◽  
Istvan Boldogh ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Human Dna ◽  
Identification And Characterization

scholarly journals Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA

2002 ◽  
Vol 99 (6) ◽  
pp. 3523-3528 ◽  
Author(s):  
Tapas K. Hazra ◽  
Tadahide Izumi ◽  
Istvan Boldogh ◽  
Barry Imhoff ◽  
Yoke W. Kow ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Human Dna ◽  
Identification And Characterization ◽  
Damaged Dna

scholarly journals Structural Characterization of Human 8-Oxoguanine DNA Glycosylase Variants Bearing Active Site Mutations

2006 ◽  
Vol 282 (12) ◽  
pp. 9182-9194 ◽  
Author(s):  
Christopher T. Radom ◽  
Anirban Banerjee ◽  
Gregory L. Verdine
Keyword(s):  
Structural Characterization ◽  
Active Site ◽  
Dna Glycosylase ◽  
Active Site Mutations

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Sign in / Sign up

Export Citation Format

Share Document