scholarly journals Multiple Mechanisms Contribute to the Activation of RNA Polymerase III Transcription in Cells Transformed by Papovaviruses

2002 ◽  
Vol 277 (50) ◽  
pp. 48182-48191 ◽  
Author(s):  
Zoë A. Felton-Edkins ◽  
Robert J. White
2020 ◽  
Vol 54 (1) ◽  
pp. 67-74 ◽  
Author(s):  
I. G. Ustyantsev ◽  
K. A. Tatosyan ◽  
D. V. Stasenko ◽  
N. Y. Kochanova ◽  
O. R. Borodulina ◽  
...  

2013 ◽  
Vol 288 (38) ◽  
pp. 27564-27570 ◽  
Author(s):  
Neha Verma ◽  
Ko-Hsuan Hung ◽  
Jin Joo Kang ◽  
Nermeen H. Barakat ◽  
William E. Stumph

In the fruit fly Drosophila melanogaster, RNA polymerase III transcription was found to be dependent not upon the canonical TATA box-binding protein (TBP) but instead upon the TBP-related factor 1 (TRF1) (Takada, S., Lis, J. T., Zhou, S., and Tjian, R. (2000) Cell 101, 459–469). Here we confirm that transcription of fly tRNA genes requires TRF1. However, we unexpectedly find that U6 snRNA gene promoters are occupied primarily by TBP in cells and that knockdown of TBP, but not TRF1, inhibits U6 transcription in cells. Moreover, U6 transcription in vitro effectively utilizes TBP, whereas TBP cannot substitute for TRF1 to promote tRNA transcription in vitro. Thus, in fruit flies, different classes of RNA polymerase III promoters differentially utilize TBP and TRF1 for the initiation of transcription.


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