scholarly journals Use of a Genetically Introduced Cross-linker to Identify Interaction Sites of Acidic Activators within Native Transcription Factor IID and SAGA

2002 ◽  
Vol 278 (9) ◽  
pp. 6779-6786 ◽  
Author(s):  
Joachim Klein ◽  
Mark Nolden ◽  
Steven L. Sanders ◽  
Jay Kirchner ◽  
P. Anthony Weil ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Interaction Sites ◽  
Cross Linker ◽  
Transcription Factor Iid

scholarly journals Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.

1991 ◽  
Vol 88 (12) ◽  
pp. 5124-5128 ◽  
Author(s):  
N. Horikoshi ◽  
K. Maguire ◽  
A. Kralli ◽  
E. Maldonado ◽  
D. Reinberg ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Direct Interaction ◽  
Tata Box ◽  
Adenovirus E1a ◽  
Transcription Factor Iid ◽  
E1a Protein

Mass-spectrometric identification of interaction sites for cytochrome P450 2b4/nadph cytochrome P450 reductase

2010 ◽  
Vol 56 (1) ◽  
pp. 40-54 ◽  
Author(s):  
A.V. Ivanov ◽  
A.T. Kopylov ◽  
V.G. Zgoda ◽  
I.Yu. Toropygin ◽  
E.V. Khrjapova ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Protein Interactions ◽  
Cytochrome P450 Reductase ◽  
Interaction Sites ◽  
Nadph Cytochrome ◽  
Protein Partners ◽  
Cytochrome P450 2B4 ◽  
Mass Spectrometric Identification ◽  
Cross Linker ◽  
Covalently Linked

We determined the interaction sites of the cytochrome P450's protein-partners: 2B4 (d-2B4) and NADPH-cytochrome P450 of reductase (d-Fp). While in operation, these proteins are forming the complexes. We used 4-4'-dithio(bisphenyl)azide linker for non-specific covalent coupling of d-2B4 complexes with d-Fp in Emulgen-913 - monomerized system. Covalently-linked peptides in this complex were identified with ESI-MS/MS. Several sites of these proteins' binding with each other were revealed. Based on them, a model of intermolecular protein interactions was created. The model includes 5 cross-linker-stabilized contact sites of d-2B4 with d-Fp involving the following peptides of d-2B4 and d-Fp: 1) d-2B4423-433 и d-Fp 102-109; 2) d-2B4324-336 и d-Fp570-585; 3) d-2B4327-336 и d-Fp452-464; 4) d-2B4 192-197 и d-Fp456-464; 5) d-2B4 134-139 и d-Fp406-425.Herein, in the latter two cases, the peptides of d-Fp are located in their inter-domain slit and stabilize protein-protein complex via nanoprobe cross-linker; therefore, the formation of d-2B4/d-Fp complexes in these sites may involve aminoacid residues d-Fp456-464 and d-Fp406-425 surrounding inter-domain slit.

Transcription factor IID probes localize a single gene to the proximal region of mouse chromosome 17

Gene ◽  
1993 ◽  
Vol 130 (2) ◽  
pp. 283-286 ◽  
Author(s):  
Michael Danciger ◽  
Christine A. Kozak ◽  
Suzi Suzuki ◽  
Mary R. Chang ◽  
Toshimichi Shinohara ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Mouse Chromosome ◽  
Single Gene ◽  
Proximal Region ◽  
Chromosome 17 ◽  
Transcription Factor Iid
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