scholarly journals Proteomic Analysis of Brain Plasma Membranes Isolated by Affinity Two-phase Partitioning

2005 ◽  
Vol 5 (2) ◽  
pp. 390-400 ◽  
Author(s):  
Jens Schindler ◽  
Urs Lewandrowski ◽  
Albert Sickmann ◽  
Eckhard Friauf ◽  
Hans Gerd Nothwang
Keyword(s):  
Proteomic Analysis ◽  
Plasma Membranes ◽  
Two Phase ◽  
Phase Partitioning

Subfractional Analysis of Cyanobacterial Membranes and Isolation of Plasma Membranes by Aqueous Polymer Two-Phase Partitioning

1994 ◽  
Vol 218 (1) ◽  
pp. 103-111 ◽  
Author(s):  
B. Norling ◽  
V. Mirzakhanian ◽  
F. Nilsson ◽  
D.J. Morre ◽  
B. Andersson
Keyword(s):  
Plasma Membranes ◽  
Two Phase ◽  
Phase Partitioning ◽  
Aqueous Polymer

scholarly journals Fractionation of rat liver plasma-membrane regions by two-phase partitioning

1986 ◽  
Vol 235 (3) ◽  
pp. 685-691 ◽  
Author(s):  
P Gierow ◽  
M Sommarin ◽  
C Larsson ◽  
B Jergil
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Plasma Membranes ◽  
Gradient Centrifugation ◽  
Fold Increase ◽  
Phase System ◽  
Two Phase ◽  
Phase Partitioning ◽  
Aqueous Polymer ◽  
Counter Current

Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential and sucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phase system. This method separates membranes according to differences in surface properties rather than size and density. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and 5′-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction, whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers. Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranes and lysosomes, showed a heterogeneous behaviour on counter-current distribution.

Aqueous Polymer Two-Phase Systems for the Proteomic Analysis of Plasma Membranes from Minute Brain Samples

2008 ◽  
Vol 7 (1) ◽  
pp. 432-442 ◽  
Author(s):  
Jens Schindler ◽  
Urs Lewandrowski ◽  
Albert Sickmann ◽  
Eckhard Friauf
Keyword(s):  
Proteomic Analysis ◽  
Plasma Membranes ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems
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