Trends of pollen grain size variation in C3 and C4 Poaceae species using pollen morphology for future assessment of grassland ecosystem dynamics

Grana ◽  
2014 ◽  
Vol 54 (2) ◽  
pp. 129-145 ◽  
Author(s):  
Farooq Jan ◽  
Lisa Schüler ◽  
Hermann Behling
2014 ◽  
Vol 65 (1-2) ◽  
pp. 111-116 ◽  
Author(s):  
Renata Śnieżko

Flowers of <em>Oenothera hookeri</em> Torr. et Gray, <em>Oe. brevistylis</em> and <em>Oe. lamarkiana</em> de Vries were pollinated after anthesis by insects. <em>Oe. biennis</em> L., <em>Oe. suaveolens</em> Desf and sulfurea were selfpollinated in the buds. Pollen morphology was slightly different: <em>Oe. hookeri</em> have regular, triporated pollen, often germinating through two pores; in <em>Oe. suaveolens</em> many pollen grains had callose patches on the intine; in <em>Oe. brevistylis</em> tetraporated pollen were more often than in other species; in <em>Oe. lamarckiana</em> many pollen grains were empty; in <em>Oe. biennis</em> and <em>Oe. suaveolens</em> pollen grain size and viability varied. The pollen tube growth and fertilization were similar in 5 species and can be considered as typical for <em>Oenothera</em>. In the ovary pollen tubes branched and changed their growth direction. Near micropyle they formed short branches to the inner integument. In the nucellus the pollen tube became swollen.


2010 ◽  
Vol 2 (4) ◽  
pp. 27-33
Author(s):  
Olubukola ADEDEJI

The exine morphology of pollen grains of Stachytarpheta indica (Linn.) Vahl, Stachytarpheta cayennensis (Rich.) Vahl and Stachytarpheta angustifolia (Mill.) Vahl is reported. This study was carried out with a light microscope. Pollen grains from fresh anthers were collected and aceolysed. Statistical analysis used to analyse the data collected include cluster analysis, correlation analysis, similarity and distance indices. The pollen grains are spheroidal to oblate to sub-oblate in shape. They are aperturate, both colpate and porate. Tricolpate types occur most frequently, acolpate, monocolpate, bicolpate and tetracolpate types less frequently. The multicolpate and multiporate attributes in all the species indicate that the genus is not primitive in evolutionary history and this species probably, evolved around in the same time. According to the size, the pollen grains of the genus falls into groups permagna (pollen diameter 100-200 μm) and giganta (pollen diameter greater than 200 μm). S. cayennensis and S. anguistifolia belong to group permagna and S. indica only in the group giganta. This separates S. indica from the other two species. The large pollen grain size in the genus clearly supports the fact that the flowers in the genus are more insect-and-bird pollinated than wind pollinated. The similarity and distance indices of the species showed that S. cayennensis and S. angustifolia are the closest. S. indica is closer to S. angustifolia but farther from S. cayennensis.


1991 ◽  
Vol 69 (12) ◽  
pp. 2583-2596 ◽  
Author(s):  
R. J. Hebda ◽  
C. C. Chinnappa ◽  
B. M. Smith

Pollen grains of 12 species of western Canadian rosaceous genera, Luetkea, Oemleria, Physocarpus, and Prunus, were studied in the light microscope and scanning electron microscope. All pollen grains are produced as isopolar, radially symmetrical, usually tricolporate monads. Grains are predominantly spheroidal with a circular to triangular amb. Most of the species exhibit a well-developed chambered aperture complex at the equator, formed by a pair of sexinal flaps whereas in Prunus spinosa, Prunus americana, and Prunus virginiana the chamber is absent or weakly developed. Oemleria cerasiformis has no pore flaps. Apertures of Prunus emarginata and Prunus virginiana often have an equatorial bridge over the aperture complex. The exine of Prunus, Physocarpus, and Luetkea pollen is tectate perforate, like that of many Rosaceae. Sculpturing consists of ridges and valleys that form a striate to rugulate pattern. Ridges and valleys tend to be mainly parallel to the colpus but often loop near the poles. Pollen grains of Prunus spinosa are always rugulate or vermiculate with ridges arranged randomly. Oemleria cerasiformis pollen appears tectate imperforate. Ridges and valleys intersect at triple points in the subpolar region and curve in a semicircle around the pore area. On this basis Oemleria pollen is distinct from all other western Canadian Rosaceae. Pollen grain size ranges from small for Luetkea pectinata (17 μm in diameter) to medium for Prunus spinosa (36 × 33 μm). Key words: pollen morphology, Rosaceae, Western Canada, systematics.


2020 ◽  
Vol 42 ◽  
pp. e46
Author(s):  
Jéssica Mena Barreto De Freitas ◽  
Andrielle Wouters Kuhn ◽  
Viviane Dal-Souto Frescura ◽  
Liliana Essi ◽  
Solange Bosio Tedesco

The objective of this study was to evaluate stomatal and pollen grain size and to estimate pollen viability of individuals from different populations of Paspalum rawitscheri (Parodi) Chase ex G.H. Rua Valls. To analyze stomatal size, slides were made of the adaxial leaf epidermis using the epidermal impression method. The height and width of 100 stomata per population were analyzed. Pollen was obtained from inflorescences to evaluate pollen grain size and pollen viability. Pollen grains were stained with 2% acetic orcein, 2% acetic carmine, or Alexander’s reactive stain. Per population, 1600 grains of pollen were observed for viability, and 50 grains of pollen were measured. There were significant differences between populations in stomatal height and pollen grain height and width. The populations also differed in pollen viability, with the Santa Maria population showing the lowest viability. The differences in stomatal and pollen grain size suggest genetic variability in the evaluated populations. Moreover, low pollen viability in one population indicates that its decline may be related to low fertility. Keywords: Grass. Ploidy. Fertility. Threatened species.


2011 ◽  
Vol 48 (No. 8) ◽  
pp. 368-375
Author(s):  
Tejaswini

Present study focuses on making best possible use of male gametophytic generation in carnation breeding program. Exploration of pollen population revealed the existence of variability in terms of pollen morphology and histochemical content among as well as within varieties and species of Dianthus caryophyllus and D. chinensis sufficient to make selection. Pollen grain size and histochemical content were found to be associated with germination capacity and pollen tube growth rate. In addition, pollen germination capacity and elongation of pollen tube in response to presence of culture filtrate from F. oxysporum. f.sp. dianthi causal organism of fusarium wilt in carnation was found to be governed by pollen grain size and histochemical content of pollen grains. Entire result suggests the possibility of selecting the desired pollen grains from a&nbsp;pollen population and possibility of attempting selective pollination in carnation breeding program.


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