Oxidative and anti-oxidative responses to metal toxicity in an extremophilic alga (Cyanidium caldarium) and a neutrophilic alga (Chlamydomonas reinhardtii)

Phycologia ◽  
2021 ◽  
pp. 1-11
Author(s):  
Paulina Mikulic ◽  
John Beardall
Author(s):  
M. R. Edwards ◽  
J. D. Mainwaring

Although the general ultrastructure of Cyanidium caldarium, an acidophilic, thermophilic alga of questionable taxonomic rank, has been extensively studied (see review of literature in reference 1), some peculiar ultrastructural features of the chloroplast of this alga have not been noted by other investigators.Cells were collected and prepared for thin sections at the Yellowstone National Park and were also grown in laboratory cultures (45-52°C; pH 2-5). Fixation (glutaraldehyde-osmium), dehydration (ethanol), and embedding (Epon 812) were accomplished by standard methods. Replicas of frozenfracture d- etched cells were obtained in a Balzers apparatus. In addition, cells were examined after disruption in a French Press.


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