Effect of Temperature and Mobile Phase Composition on RP-HPLC Separation of Cephalosporins

1990 ◽  
Vol 13 (16) ◽  
pp. 3269-3288 ◽  
Author(s):  
J. Martín-Villacorta ◽  
R. Méndez
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Effect Of Temperature ◽  
Hplc Separation ◽  
Mobile Phase Composition ◽  
Rp Hplc

β-Lactam Antibiotics: Effect of Temperature and Mobile Phase Composition on Reversed-Phase HPLC Separation

2005 ◽  
pp. 178-183
Author(s):  
J. Martín-Villacorta ◽  
R. Méndez ◽  
N. Montes ◽  
J. C. García-Glez
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Effect Of Temperature ◽  
Reversed Phase Hplc ◽  
Hplc Separation ◽  
Mobile Phase Composition

Use of Three-Dimensional Minimum  -Plots for Optimization of Mobile Phase Composition for RP-HPLC Separation of Sulfonamides

1984 ◽  
Vol 22 (1) ◽  
pp. 31-39 ◽  
Author(s):  
J. W. Weyland ◽  
C. H. P. Bruins ◽  
D. A. Doornbos
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Three Dimensional ◽  
Hplc Separation ◽  
Mobile Phase Composition ◽  
Rp Hplc

Effect of chemically bonded stationary phases and mobile phase composition onβ-blockers retention in RP-HPLC

2009 ◽  
Vol 23 (3) ◽  
pp. 324-333 ◽  
Author(s):  
Boguslaw Buszewski ◽  
Tomasz Welerowicz ◽  
Tomasz Kowalkowski
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Stationary Phases ◽  
Mobile Phase Composition ◽  
Bonded Stationary Phases ◽  
Rp Hplc

scholarly journals Quantitative estimation of trimazolin hydrochloride in pharmaceutical preparation by RP-HPLC method

2009 ◽  
Vol 63 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Ivana Savic ◽  
Goran Nikolic ◽  
Vladimir Bankovic ◽  
Ivan Savic
Keyword(s):  
Phase Composition ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Quantitative Estimation ◽  
Pharmaceutical Preparation ◽  
Hplc Method ◽  
Mobile Phase Composition ◽  
Rp Hplc ◽  
Ich Guidelines

A sensitive and selective RP-HPLC method was developed and validated for the quantitative determination of trimazolin hydrochloride in nasal drops formulations. The mobile phase composition was water-acetonitrile (50:50, v/v) and the UV detection was carried out at 270 nm. Linearity range in the concentration range of 10 to 110 ?g cm-3. The method was tested and validated for various parameters according to ICH guidelines. The detection and quantization limits were found to be 1.45 and 4.8 ?g cm-3, respectively. The results demonstrated that the procedure for estimation of trimazolin hydrochloride in nasal drops formulations was accurate, precise and reproducible.

scholarly journals IMPLICATIONS OF MOBILE PHASE COMPOSITION AND PH ON THE CHROMATOGRAPHIC SEPARATION OF AMITRIPTYLINE AND ITS METABOLITE NORTRIPTYLINE

2018 ◽  
Vol 10 (4) ◽  
pp. 132
Author(s):  
Luana Mifsud Buhagiar ◽  
Manuel Scorpiniti ◽  
Nicolette Sammut Bartolo ◽  
Janis Vella Szijj ◽  
Victor Ferrito ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phase Composition ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Organic Modifier ◽  
Mobile Phase Composition ◽  
Simple Method ◽  
Column Temperature ◽  
Rp Hplc

Objective: Separation of tricyclic compounds sets the keystone for determining parent drug to metabolite concentration ratios and analysing impurities. The combined effects of acetonitrile composition and pH of the mobile phase on the separation of amitriptyline and nortriptyline by reversed-phase high-performance liquid chromatography (RP-HPLC) are presented.Methods: A series of RP-HPLC triplicate runs were carried out using acetonitrile and a phosphate buffer as the mobile phase and a Kinetex® C18 LC Column as the stationary phase using an Agilent 1260 Infinity Series® II liquid chromatography system with UV/visible detection. The stationary phase, column temperature, injection volume and flow rate were kept unchanged during analysis. Mobile phase composition and pH were varied to observe impact on peak shape, resolution and retention time, taking into consideration green analytical chemistry aspects.Results: Optimal chromatographic outcomes were achieved when using the mobile phase made up of 35% acetonitrile and 65% buffer at a pH of 5.6. These conditions resulted in nortriptyline and amitriptyline eluting at 4.66 min and 5.92 min respectively. Increasing the organic modifier content of the mobile phase to 40% completed separation within a run time of 4 min with comparable resolution. The 2 min gained by increasing 5% acetonitrile may not be justified due to potential implications on greening laboratory practices.Conclusion: Reversed-phase chromatography embodies a simple method for the separation of compounds that are similar in structure. Attuning the percentage of organic modifier and buffer pH provides acceptable retention times, without compromising resolution between neighbouring peaks.

EFFECT OF MOBILE PHASE COMPOSITION AND pH ON HPLC SEPARATION OF RHIZOME OF POLYGONUM BISTORTA

2012 ◽  
Vol 35 (7) ◽  
pp. 977-987 ◽  
Author(s):  
Azeem Intisar ◽  
J. Boima Kiazolu ◽  
Yun Wang ◽  
Lingyi Zhang ◽  
Weibing Zhang
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Hplc Separation ◽  
Mobile Phase Composition
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