Anti‐viral immunity against Marek's disease virus infected chicken kidney cells

Abstract Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.

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Inhibitory Effects of Macrophages Against Marek's Disease Virus Plaque Formation in Chicken Kidney Cell Cultures2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/63.5.1267 ◽

1979 ◽

Keyword(s):

Disease Virus ◽

Cell Cultures ◽

Kidney Cell ◽

Marek's Disease Virus ◽

Plaque Formation ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Inhibitory Effects ◽

Chicken Kidney

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Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA

10.21203/rs.2.15458/v1 ◽

2019 ◽

Author(s):

Ke Ding ◽

Zuhua Yu ◽

Chuan Yu ◽

Yanyan Jia ◽

Lei He ◽

...

Keyword(s):

Disease Virus ◽

Tumor Suppressors ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Infected Chicken ◽

Exact Role ◽

Transformed Cell Line ◽

Underlying Mechanisms

Abstract Background: Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. Results: The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targetedthe RORA gene and downregulated its expression in the MSB1 cells.Conclusion: The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.

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Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA

10.21203/rs.2.15458/v2 ◽

2019 ◽

Author(s):

Ke Ding ◽

Zuhua Yu ◽

Chuan Yu ◽

Yanyan Jia ◽

Lei He ◽

...

Keyword(s):

Disease Virus ◽

Tumor Suppressors ◽

Marek's Disease Virus ◽

Marek's Disease ◽

G1 Phase ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Infected Chicken ◽

Exact Role ◽

Transformed Cell Line

Abstract Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.

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Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA

10.21203/rs.2.15458/v4 ◽

2020 ◽

Author(s):

Ke Ding ◽

Zuhua Yu ◽

Chuan Yu ◽

Yanyan Jia ◽

Lei He ◽

...

Keyword(s):

Disease Virus ◽

Tumor Suppressors ◽

Marek's Disease Virus ◽

Marek's Disease ◽

G1 Phase ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Infected Chicken ◽

Exact Role ◽

Transformed Cell Line

Abstract Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.

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Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.22.102 ◽

2019 ◽

Vol 20 (2) ◽

pp. 6-11

Author(s):

Aly El-Kenawy ◽

Mohamed El-Tholoth ◽

Emad A

Keyword(s):

Real Time ◽

Disease Virus ◽

Real Time Pcr ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Field Samples ◽

Feather Follicle ◽

Positive Results

In the present study, a total of 16 samples including feather follicle epithelium, ovary, spleen and kidney (4 samples for each organ) were collected from diseased chicken flocks suspected to be infected with Marek’s disease virus (MDV) at Dakahlia Governorate, Egypt during the period from October 2016 to October 2017. Each sample was pooled randomly from three to five birds (90 to 360 days old). The isolation of the suspected virus from the collected samples was carried out via chorioallantoic membranes (CAMs) of 12 days old embryonated chicken eggs (ECEs). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard MDV. MDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). Molecular identification of virus was carried out by conventional polymerase chain reaction (PCR) and real- time PCR in four selected samples. The results revealed that 14 samples (87.5%) including 4 (100%) samples from feather follicle epithelium, ovary and kidney and 2 (50%) samples from spleen, showed positive results in virus isolation after 3rd passage. The positive results percentage by AGPT for field samples were 50% (8 out of 16 samples), while after the 3rd passage in ECEs were 37.5% (6 out of 16 samples) and the positive results percentage by IFAT for field samples were 62.5% (10 out of 16 samples), while after the 3rd passage in ECEs were 81.25 % (13 out of 16 samples). Viral nucleic acid was detected in all selected samples by conventional and real- time PCR. The results indicate that feather follicle epithelium is the best organ for MDV detection. IFAT is superior over AGPT in virus detection. Conventional and real - time PCR could be efficiently used for molecular detection of the virus.

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