Probing Site-specific Interactions in Protein-DNA Complexes Using Heteronuclear NMR Spectroscopy and Molecular Modeling: Binding of Cro Repressor to OR3

1998 ◽  
Vol 16 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Carol A. Edwards ◽  
Chang-Shung Tung ◽  
Louis A. Silks ◽  
Joe M. Gatewood ◽  
James A. Fee ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectroscopy ◽  
Heteronuclear Nmr ◽  
Specific Interactions ◽  
Dna Complexes ◽  
Site Specific ◽  
Heteronuclear Nmr Spectroscopy ◽  
Cro Repressor

Solution of the spatial structure of dimeric transmembrane domains of proteins by heteronuclear NMR spectroscopy and molecular modeling

BIOPHYSICS ◽  
2006 ◽  
Vol 51 (S1) ◽  
pp. 23-27 ◽  
Author(s):  
P. E. Volynsky ◽  
E. V. Bocharov ◽  
D. E. Nolde ◽  
Ya. A. Vereschaga ◽  
M. L. Mayzel ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectroscopy ◽  
Spatial Structure ◽  
Heteronuclear Nmr ◽  
Transmembrane Domains ◽  
Heteronuclear Nmr Spectroscopy

scholarly journals Structure-Functional Insight into Transmembrane Helix Dimerization by Protein Engineering, Molecular Modeling and Heteronuclear NMR Spectroscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 470a ◽  
Author(s):  
Eduard Bocharov ◽  
Pavel Volynsky ◽  
Konstantin Mineev ◽  
Dmitry Lesovoy ◽  
Kirill Nadezhdin ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectroscopy ◽  
Protein Engineering ◽  
Heteronuclear Nmr ◽  
Transmembrane Helix ◽  
Heteronuclear Nmr Spectroscopy ◽  
Insight Into

Molecular modulation of calcium oxalate crystallization

2006 ◽  
Vol 291 (6) ◽  
pp. F1123-F1132 ◽  
Author(s):  
James J. De Yoreo ◽  
S. Roger Qiu ◽  
John R. Hoyer
Keyword(s):  
Molecular Modeling ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Energy Levels ◽  
Critical Role ◽  
Specific Interactions ◽  
Crystal Surfaces ◽  
Site Specific

Calcium oxalate monohydrate (COM) is the primary constituent of the majority of renal stones. Osteopontin (OPN), an aspartic acid-rich urinary protein, and citrate, a much smaller molecule, are potent inhibitors of COM crystallization at levels present in normal urine. Current concepts of the role of site-specific interactions in crystallization derived from studies of biomineralization are reviewed to provide a context for understanding modulation of COM growth at a molecular level. Results from in situ atomic force microscopy (AFM) analyses of the effects of citrate and OPN on growth verified the critical role of site-specific interactions between these growth modulators and individual steps on COM crystal surfaces. Molecular modeling investigations of interactions of citrate with steps and faces on COM crystal surfaces provided links between the stereochemistry of interaction and the binding energy levels that underlie mechanisms of growth modification and changes in overall crystal morphology. The combination of in situ AFM and molecular modeling provides new knowledge that will aid rationale design of therapeutic agents for inhibition of stone formation.

Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids

2019 ◽  
Vol 305 ◽  
pp. 146-151 ◽  
Author(s):  
Thomas G. Meikle ◽  
Ashish Sethi ◽  
David W. Keizer ◽  
Jeffrey J. Babon ◽  
Frances Separovic ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Heteronuclear Nmr ◽  
Cubic Phase ◽  
Heteronuclear Nmr Spectroscopy
Sign in / Sign up

Export Citation Format

Share Document