Enamel matrix proteins regulate hypoxia-induced cellular biobehavior and osteogenic differentiation in human periodontal ligament cells

2017 ◽  
Vol 92 (8) ◽  
pp. 606-618 ◽  
Author(s):  
ZC Song ◽  
S Li ◽  
JC Dong ◽  
MJ Sun ◽  
XL Zhang ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Human Periodontal Ligament Cells

scholarly journals Interaction of enamel matrix proteins with human periodontal ligament cells

2015 ◽  
Vol 20 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Harsh D. Amin ◽  
Irwin Olsen ◽  
Jonathan Knowles ◽  
Michel Dard ◽  
Nikolaos Donos
Keyword(s):  
Periodontal Ligament ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Human Periodontal Ligament Cells

Effects of enamel matrix proteins on multi-lineage differentiation of periodontal ligament cells in vitro

2013 ◽  
Vol 9 (1) ◽  
pp. 4796-4805 ◽  
Author(s):  
Harsh D. Amin ◽  
Irwin Olsen ◽  
Jonathan C. Knowles ◽  
Michel Dard ◽  
Nikolaos Donos
Keyword(s):  
Periodontal Ligament ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Lineage Differentiation

Expression Profiling of Periodontal Ligament Cells Stimulated with Enamel Matrix Proteins in vitro: A Model for Tissue Regeneration

2002 ◽  
Vol 81 (11) ◽  
pp. 776-783 ◽  
Author(s):  
P.M. Brett ◽  
M. Parkar ◽  
I. Olsen ◽  
M. Tonetti
Keyword(s):  
Periodontal Ligament ◽  
Gene Array ◽  
Periodontal Regeneration ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Genetic Responses

Several studies have examined the role of enamel matrix proteins in root formation and periodontal regeneration, although most of these have focused on a few specific genes which had previously been implicated. However, recent advances in expressional profiling have made it possible to examine the range of genetic responses involved in these processes. In the present experiments, we have therefore utilized this technique to determine the effects of enamel matrix proteins on the gene activities of periodontal ligament cells in vitro. Such cells were found to have an elevated level of RNA synthesis compared with control cells. Moreover, hybridization of the cDNA prepared from this RNA to gene array filters showed that there was differential expression of 121 genes, most of which had not previously been associated with periodontal regeneration. Some of these selective changes in gene activity might thus reflect the fundamental events that underlie periodontal development.

Gene profile in periodontal ligament cells and clones with enamel matrix proteins derivative

2007 ◽  
Vol 34 (7) ◽  
pp. 599-609 ◽  
Author(s):  
Idit Barkana ◽  
Eleni Alexopoulou ◽  
Shoshi Ziv ◽  
Jasmin Jacob-Hirsch ◽  
Ninette Amariglio ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Gene Profile ◽  
Enamel Matrix Proteins

scholarly journals Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway

2021 ◽  
Vol 9 ◽  
Author(s):  
Lingjun Li ◽  
Yangheng Zhang ◽  
Min Wang ◽  
Jing Zhou ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Osteogenic Differentiation ◽  
P38 Mapk ◽  
Periodontal Ligament ◽  
Mapk Pathway ◽  
Periodontal Regeneration ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
P38 Mapk Pathway

Periodontitis is a chronic inflammatory disease with plaques as the initiating factor, which will induce the destruction of periodontal tissues. Numerous studies focused on how to obtain periodontal tissue regeneration in inflammatory environments. Previous studies have reported adenovirus-mediated human β-defensin 3 (hBD3) gene transfer could potentially enhance the osteogenic differentiation of human periodontal ligament cells (hPDLCs) and bone repair in periodontitis. Gold nanoparticles (AuNPs), the ideal inorganic nanomaterials in biomedicine applications, were proved to have synergetic effects with gene transfection. To further observe the potential promoting effects, AuNPs were added to the transfected cells. The results showed the positive effects of osteogenic differentiation while applying AuNPs into hPDLCs transfected by adenovirus encoding hBD3 gene. In vivo, after rat periodontal ligament cell (rPDLC) transplantation into SD rats with periodontitis, AuNPs combined hBD3 gene modification could also promote periodontal regeneration. The p38 mitogen-activated protein kinase (MAPK) pathway was demonstrated to potentially regulate both the in vitro and in vivo processes. In conclusion, AuNPs can promote the osteogenic differentiation of hBD3 gene-modified hPDLCs and periodontal regeneration via the p38 MAPK pathway.

Sign in / Sign up

Export Citation Format

Share Document