Production and characterization of a fusion form of hepatitis E virus tORF2 capsid protein in Escherichia coli

2020 ◽  
pp. 1-8
Author(s):  
Mohamed Boumaiza ◽  
Khaled Trabelsi ◽  
Zeineb Choucha ◽  
Ines Akrouti ◽  
Serena Leone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E

Characterization of capsid protein (p495) of hepatitis E virus expressed in Escherichia coli and assembling into particles in vitro

Vaccine ◽  
2018 ◽  
Vol 36 (16) ◽  
pp. 2104-2111 ◽  
Author(s):  
Minghua Zheng ◽  
Jie Jiang ◽  
Xiao Zhang ◽  
Nan Wang ◽  
Kaihang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E

Characterization of monoclonal antibodies to hepatitis E virus (HEV) capsid protein and identification of binding activity

2007 ◽  
Vol 14 (5) ◽  
pp. 555-563 ◽  
Author(s):  
Junkun He ◽  
Robert A. Kuschner ◽  
Vincent Dewar ◽  
Pierre Voet ◽  
Ludmila V. Asher ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Binding Activity

Production of capsid proteins of rat hepatitis E virus in Escherichia coli and characterization of self-assembled virus-like particles

2021 ◽  
pp. 198483
Author(s):  
Tominari Kobayashi ◽  
Masaharu Takahashi ◽  
Satoshi Ohta ◽  
Shigeo Nagashima ◽  
Putu Prathiwi Primadharsini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Capsid Proteins ◽  
Virus Like Particles ◽  
Self Assembled

Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles

2017 ◽  
Vol 102 (1) ◽  
pp. 185-198 ◽  
Author(s):  
Martynas Simanavicius ◽  
Paulius Lukas Tamosiunas ◽  
Rasa Petraityte-Burneikiene ◽  
Reimar Johne ◽  
Rainer G. Ulrich ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Capsid ◽  
Virus Like Particles ◽  
Antigenic Characterization ◽  
Virus Capsid Protein

scholarly journals Antigenic Characterization of ORF2 and ORF3 Proteins of Hepatitis E Virus (HEV)

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigen ◽  
Open Reading Frame ◽  
Reading Frame ◽  
E Coli ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Open Reading Frame 2

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization

scholarly journals Prevalence and Molecular Characterization of the Hepatitis E Virus in Retail Pork Products Marketed in Canada

2017 ◽  
Vol 9 (2) ◽  
pp. 208-218 ◽  
Author(s):  
Oksana Mykytczuk ◽  
Jennifer Harlow ◽  
Sabah Bidawid ◽  
Nathalie Corneau ◽  
Neda Nasheri
Keyword(s):  
Molecular Characterization ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pork Products

Identification and characterization of avian hepatitis E virus genotype 2 from chickens with hepatitis-splenomegaly syndrome in Korea

Virus Genes ◽  
2016 ◽  
Vol 52 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Hyun-Woo Moon ◽  
Byung-Woo Lee ◽  
Haan Woo Sung ◽  
Byung-Il Yoon ◽  
Hyuk Moo Kwon
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 2 ◽  
Avian Hepatitis E Virus ◽  
Identification And Characterization

scholarly journals Identification of two neutralization epitopes on the capsid protein of avian hepatitis E virus

2008 ◽  
Vol 89 (2) ◽  
pp. 500-508 ◽  
Author(s):  
E.-M. Zhou ◽  
H. Guo ◽  
F. F. Huang ◽  
Z. F. Sun ◽  
X. J. Meng
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus
Sign in / Sign up

Export Citation Format

Share Document