Determination of Girisopam (2,3-Benzodiazepine Compound) and Its Four Metabolites in Human and Rat Plasma by Gradient RP-HPLC Method

1998 ◽  
Vol 21 (6) ◽  
pp. 803-818 ◽  
Author(s):  
I. Ürmös ◽  
I. Klebovich ◽  
K. B. Nemes
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

Ecofriendly chromatographic methods for determination of co-prescribed drugs, olanzapine and metformin, in rat plasma

Bioanalysis ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 597-613
Author(s):  
Aml A Emam ◽  
Neven M Habib ◽  
Hamada M Mahmoud ◽  
Nada S Abdelwhab ◽  
Maha M Abdelrahman
Keyword(s):  
Pharmaceutical Preparation ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hypoglycemic Agent ◽  
Chromatographic Methods ◽  
Rp Hplc ◽  
Development And Validation ◽  
Developing System

Background: Olanzapine (OLZ) is one of most recommended drugs for the treatment of schizophrenia while metformin (MET) is the most commonly used hypoglycemic agent. Aim: Development and validation of two green, sensitive and accurate chromatographic methods for the simultaneous determination of OLZ along with the co-prescribed, MET. Materials & methods: TLC-densitometric method with a developing system consisting of methylene chloride:methanol:ethyl acetate:triethylamine (4:4:5:0.1, by volume) and a reversed-phase (RP)-HPLC method where the chromatographic separation was performed using ethanol:water mixture (50: 50, v/v) as a mobile phase. Results: TLC-densitometric method had linearity over concentration ranges of 160–4000 ng/band for OLZ and 150–4500 ng/band for MET, while RP-HPLC method was linear and validated over concentration range of 300–20000 ng/ml for OLZ and MET. Conclusion: Pharmacokinetic study was successfully performed and suggested the possibility of co-administration of MET with OLZ and their further formulation in one pharmaceutical preparation to enhance patient’s compliance.

A simplified and sensitive validated RP-HPLC method for determination of temozolomide in rat plasma and its application to a pharmacokinetic study

2018 ◽  
Vol 41 (10) ◽  
pp. 692-697 ◽  
Author(s):  
Archana Khosa ◽  
Kowthavarapu V. Krishna ◽  
Ranendra N. Saha ◽  
Sunil K. Dubey ◽  
Satish Reddi
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

scholarly journals Development of RP-HPLC method for simultaneous determination of docetaxel and curcumin in rat plasma: Validation and stability

2017 ◽  
Vol 12 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Dong Wuk Kim ◽  
Abid Mehmood Yousaf ◽  
Dong Xun Li ◽  
Jong Oh Kim ◽  
Chul Soon Yong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

ESTIMATION OF TINIDAZOLE IN ALBINO RAT PLASMA BY USING RP-HPLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (06) ◽  
pp. 48-52
Author(s):  
M. Debnath ◽  
◽  
Y. Indira Muzib ◽  
S. Ashutosh Kumar
Keyword(s):  
Mobile Phase ◽  
Potassium Dihydrogen Phosphate ◽  
High Specificity ◽  
Rat Plasma ◽  
Hplc Method ◽  
Precipitation Method ◽  
Dihydrogen Phosphate ◽  
Albino Rat ◽  
Rp Hplc

A new RP-HPLC method for the quantitative determination of tinidazole in albino rat plasma was developed and validated as per US-FDA guidelines. The drug was spiked in the albino rat plasma and extracted with mobile phase by precipitation method. The extracted analyte was injected into Hypersil ODS C 18 (4.6 x 150 mm; 5 μm) or equivalent, maintained at 25°C temperature and effluent monitored at 310 nm. The mobile phase consisted of potassium dihydrogen phosphate [pH 3.0]: acetonitrile [HPLC Grade] (40:60 v/v). The flow rate was maintained at 1.0 mL/min. The developed method shows high specificity for tinidazole. The calibration curve for tinidazole was linear from 1.0 to 40.0 ng/0.5mL plasma (r 2 = 1). The inter-day and intra-day precision was found to be within limits. The average % recovery for the drug tinidazole was found to be 99.53 - 100.21 % and the reproducibility was found to be satisfactory. The proposed method was adequate, sensitivity, reproducibility, and specificity for the determination of tinidazole in albino rat plasma.

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