chromatographic methods
Recently Published Documents


TOTAL DOCUMENTS

1339
(FIVE YEARS 154)

H-INDEX

53
(FIVE YEARS 6)

ADMET & DMPK ◽  
2022 ◽  
Author(s):  
Seth K. Amponsah ◽  
Joseph A. Boadu ◽  
Daniel K. Dwamena ◽  
Kwabena F.M. Opuni

Aminoglycosides are broad-spectrum antibiotics used in the treatment of gram-negative bacterial infections. Due to their nephrotoxic and ototoxic potential (narrow therapeutic index), the use of aminoglycoside for clinical indications requires monitoring. The objective of this review was to identify relevant literature reporting liquid chromatographic methods for the bioanalysis of aminoglycosides in both preclinical and clinical settings/experiments. Data on liquid chromatographic methods were collected from articles in an online academic database (PubMed, Science Direct, Scopus, and Google Scholar). All 71 articles published from 1977 to 2020 were included in the review. Reversed-phase liquid chromatography was the most used method for the bioanalysis of aminoglycosides. Fluorescence or ultraviolet detection methods were mostly used from 1977 to 2002 (51 articles), while mass spectrometry was predominantly used as a detector from 2003 to 2020 (15 articles). Sixty-seven articles reported calibration ranges, which varied significantly for the various drugs assayed: some in the range of 0.1-0.5 ng/mL and others 1250-200000 ng/mL. Also, 61 articles reported R2 values (0.964-1.0) for almost all analytes under consideration. Sixty-three articles reported percent recoveries mostly between 61.0 % to 114.0 %, with only two articles reporting recoveries of 4.9 % and 36 %. Out of the 71 reviewed articles, 56 reported intermediate precision values ranging between 0.331 % to 19.76 %, which is within the acceptable limit of 20 %. This review will serve as a guide for research and/or routine clinical monitoring of aminoglycosides in biological matrices.


2022 ◽  
pp. 32-92
Author(s):  
Mario Contin ◽  
Cecilia Dobrecky ◽  
Sabrina Flor ◽  
Manuela Martinefski ◽  
Silvia Lucangioli ◽  
...  

2021 ◽  
Vol 14 (4) ◽  
pp. 489-501
Author(s):  
Mikhail V. Shashkov ◽  
◽  
Yulia S. Sotnikova ◽  
Pavel A. Dolgushev ◽  
Maria V. Alekseeva

This paper presents a study of the pyrolysis products organic raw materials (bio-oil and sludge sediments of treatment facilities) by chromatographic methods. A feature of the work is to optimize the sample preparation procedure by fractionating the pyrolysis products. Using the method of gel permeation chromatography, molecular weight distribution of pyrolysis products was assessed. Determination of the water content in these objects (by Karl Fischer titration) was used to assess the possibility of their direct analysis by gas chromatography. A sample of sludge pyrolysis and several fractions obtained from a bio-oil sample were analyzed. By the method of two-dimensional gas chromatography, where a selfdeveloped column based on an ionic liquid was used as the first measurement column, the pyrolysate of sludge sediments and the ether fraction of bio-oil were analyzed. The obtained chromatograms and quantitative results are presented


Sign in / Sign up

Export Citation Format

Share Document