scholarly journals COMPARATIVE STUDIES IN ROUS SARCOMA WITH VIRUS, TUMOR CELLS, AND CHICK EMBRYO CELLS TRANSFORMED IN VITRO BY VIRUS

1961 ◽  
Vol 114 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Stian Erichsen ◽  
Jan Eng ◽  
Herbert R. Morgan
Keyword(s):  
Chick Embryo ◽  
Tumor Cells ◽  
Rous Sarcoma Virus ◽  
Chick Embryo Fibroblast ◽  
Transformed Cell ◽  
Rous Sarcoma ◽  
Embryo Cells ◽  
Sarcoma Virus

The chick embryo fibroblast infected by Rous sarcoma virus in vitro acquires the capacity to produce the acid mucopolysaccharides which are found in the tumors caused by this virus and which are also produced by tumor cells in vitro. The transformed cell acquires synthetic as well as morphologic, metabolic, and proliferative properties characteristic of Rous sarcoma tumor cells in vivo and in vitro and the transformed cell may be analogous to the tumor cell produced by virus infection in vivo.

scholarly journals COMPARATIVE STUDIES IN ROUS SARCOMA WITH VIRUS, TUMOR CELLS, AND CHICK EMBRYO CELLS TRANSFORMED IN VITRO BY VIRUS

1962 ◽  
Vol 115 (1) ◽  
pp. 245-251 ◽  
Author(s):  
Robert M. Dougherty ◽  
Herbert R. Morgan
Keyword(s):  
Chick Embryo ◽  
Tumor Cells ◽  
Comparative Studies ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Transformed Fibroblasts ◽  
Embryo Cells ◽  
Sarcoma Virus

Chick embryo fibroblasts infected in vitro with Rous sarcoma virus have properties similar to tumor cells when injected into virus-immune chickens. When such virus-transformed fibroblasts are injected into normal chickens, they apparently participate in the production of tumors independent of their release of virus and are thus apparently malignant in vivo.

Growth curve of Rous sarcoma virus on chick embryo cells in vitro

Virology ◽  
1959 ◽  
Vol 8 (1) ◽  
pp. 60-79 ◽  
Author(s):  
Philippe Vigier ◽  
Alice Goldé
Keyword(s):  
Chick Embryo ◽  
Growth Curve ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Embryo Cells ◽  
Sarcoma Virus

Functional domains of the pp60v-src protein as revealed by analysis of temperature-sensitive Rous sarcoma virus mutants

1984 ◽  
Vol 4 (8) ◽  
pp. 1508-1514
Author(s):  
A W Stoker ◽  
P J Enrietto ◽  
J A Wyke
Keyword(s):  
Rous Sarcoma Virus ◽  
Kinase Activity ◽  
Temperature Sensitive ◽  
Tyrosine Kinase Activity ◽  
Rous Sarcoma ◽  
Heat Labile ◽  
Src Gene ◽  
Sarcoma Virus

Four temperature-sensitive (ts) Rous sarcoma virus src gene mutants with lesions in different parts of the gene represent three classes of alteration in pp60src. These classes are composed of mutants with (i) heat-labile protein kinase activities both in vitro and in vivo (tsLA27 and tsLA29), (ii) heat-labile kinases in vivo but not in vitro (tsLA33), and (iii) neither in vivo nor in vitro heat-labile kinases (tsLA32). The latter class indicates the existence of structural or functional pp60src domains that are required for transformation but do not grossly affect tyrosine kinase activity.

Long-term cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain (D) of Rous sarcoma virus

1976 ◽  
Vol 22 (10) ◽  
pp. 1474-1479
Author(s):  
Lorraine Leblond-Larouche ◽  
Réjean Morais
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Viable Cells ◽  
Deficient Medium ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Roller Cultures

Attempts have been made to keep in vitro, for extended periods of time, cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain of Rous sarcoma virus, subgroup D. Roller cultures of transformed chick cells kept in serum-deficient medium can be maintained without subcultivation for up to 6 months. The confluent cultures continuously release viruses and viable tumor cells into the medium. The released cells can be plated and have characteristics of growth and morphology which are relatively stable with time until the culture degenerates. Cells released at later stages of the culture produced substantially more viruses than those released earlier, suggesting that cell selection or differentiation occurs during long-term cultivation in low serum concentration. Long-term cultures of untransformed chick embryo fibroblasts can also be maintained in the same way. The release of viable cells by these confluent cultures, however, is negligible.

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