scholarly journals EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT)

1965 ◽  
Vol 121 (6) ◽  
pp. 1071-1086 ◽  
Author(s):  
Adnan S. Dajani ◽  
Wallace A. Clyde ◽  
Floyd W. Denny
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Fluorescent Antibody ◽  
Indirect Fluorescent Antibody ◽  
Quantitative Culture ◽  
Culture Methods ◽  
Qualitative And Quantitative ◽  
Antibody Staining ◽  
Colony Forming Units ◽  
Mycoplasma Pneumoniae Infection ◽  
Fluorescent Antibody Staining

The pathogenesis of Mycoplasma pneumoniae infection was studied in the Syrian hamster with qualitative and quantitative culture methods and special histopathologic techniques. The animals were readily infected with the mycoplasma, which multiplied throughout the respiratory tract. Sensitivity of this experimental host to infection was indicated by the 50 per cent infective dose, which was 10 colony-forming units of the organism. Inoculation consistently resulted in the production of peribronchial pneumonitis which was induced by the mycoplasma. The organisms were visualized in a superficial location in the mucosa of involved bronchi, by means of indirect fluorescent antibody staining and by a modification of the Brown and Brenn technique. The data indicate applicability of the hamster to the study of problems concerned with M. pneumoniae disease which are impractical or impossible to resolve in the human host.

scholarly journals Quantitative immunohistochemistry: a comparison of microdensitometric analysis of unlabeled antibody peroxidase-antiperoxidase staining and of microfluorometric analysis of indirect fluorescent antibody staining for nicotinamide adenosine dinucleotide phosphate (NADPH)-cytochrome c (P-450) reductase in rat liver.

1983 ◽  
Vol 31 (10) ◽  
pp. 1183-1189 ◽  
Author(s):  
M T Smith ◽  
J A Redick ◽  
J Baron
Keyword(s):  
Cytochrome C ◽  
Rat Liver ◽  
Immunohistochemical Staining ◽  
Fluorescent Antibody ◽  
Indirect Fluorescent Antibody ◽  
Liver Lobule ◽  
Nadph Cytochrome ◽  
Antibody Staining ◽  
Unlabeled Antibody ◽  
Fluorescent Antibody Staining

The intralobular distribution of nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c (P-450) reductase (NADPH:ferricytochrome oxidoreductase, EC 1.6.2.4) in rat liver has been investigated by means of two quantitative immunohistochemical techniques: microdensitometric quantitation of unlabeled antibody peroxidase-antiperoxidase staining and microfluorometric analysis of indirect fluorescent antibody staining. Utilizing sheep antiserum elicited against NADPH-cytochrome c (P-450) reductase that had been isolated and purified to apparent homogeneity from rat liver microsomes, the reductase was detected within hepatocytes throughout the liver. However, differences in the intensity of staining of hepatocytes within different regions of the liver lobule were readily apparent after completion of both immunohistochemical staining procedures. These visual findings were verified by microdensitometric and microfluorometric analyses of immunohistochemical staining, both of which revealed that approximately the same degree of staining for NADPH-cytochrome c (P-450) reductase was produced within the centrilobular and midzonal regions of the liver lobule, whereas periportal hepatocytes were stained with significantly less intensity. These results demonstrate that the application of either microdensitometry in conjunction with unlabeled antibody peroxidase-antiperoxidase staining or microfluorometry after indirect fluorescent antibody staining can be used to quantitatively determine the intratissue distributions of antigens.

scholarly journals Experimental Brucella Abortus Strain 19 Arthritis in Young Cattle

1994 ◽  
Vol 6 (1) ◽  
pp. 56-61 ◽  
Author(s):  
B. Johnson ◽  
D. A. Mosier ◽  
R. J. Morton ◽  
A. W. Confer
Keyword(s):  
Brucella Abortus ◽  
Fluorescent Antibody ◽  
Post Inoculation ◽  
Antibody Staining ◽  
Transmission Electron ◽  
Colony Forming Units ◽  
Chronic Synovitis ◽  
Young Cattle ◽  
Culture Negative ◽  
Fluorescent Antibody Staining

Several reports have shown an association between lameness in cattle and vaccination with Brucella abortus strain 19. Affected joints are culture negative for Brucella, but the synovial fluid is positive for B. abortus antibodies. The joints contain cloudy fluid, with villous proliferation of the synovium. Brucella abortus antigens are often found in the synovium with fluorescent antibody staining. This report describes the experimental reproduction of a chronic synovitis in 6 young Angus steers using intra-articular injections of B. abortus strain 19. The carpal and tibial joints were injected with 5 × 109 colony-forming units/ml of B. abortus strain 19 and regularly biopsied over a 28-day period. Steers started becoming serologically positive for B. abortus on post-inoculation day (PID) 5 and were all positive by PID 7. Joints were cultured and examined by fluorescent antibody staining, immunohistochemical methods, and light and transmission electron microscopy. Lesions typical of the field cases were present by PID 21. Brucella abortus was cultured more often during PID 1–5 (6 of 9 joints) than during PID 7–28 (3 of 15 joints). Brucella abortus was only found on PID 1, and 5 by fluorescent antibody staining and in only 2 joints immunohistochemically on PID 5 and 7. The reproduction of lesions typical of field cases but the inability to locate B. abortus antigens in the synovium raises the question of whether in field cases the synovium is continually or intermittently seeded with bacteria or if factors other than just the bacterium are needed to perpetuate the lesion.

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