Growing of replacement young cattle in ООО «PskovAgroInvest»

ООО «PskovAgroInvest» pays great attention to the rearing of replacement young animals. The rate of increase in the intensity of raising heifers on the farm meets the standards and requirements of pedigree dairy cattle breeding. The farm uses stage-bystage rearing of replacement young stock, taking into account its age. A group approach to rearing young animals. Replacement heifers use natural and cultivated pastures in the summer. In recent years, the age of replacement heifers at the first insemination has noticeably decreased and approached the norm. At the same time, the live weight of animals at the first insemination increased, which in 2020 amounted to 460 kg, which opens up prospects for a further decrease in the age of the first insemination. This became possible due to an increase in the average daily growth in cultivation, which regularly increases every year and amounts to 756 g in 2020, which is 98 g, or 14.9%, more than in 2016. From table 2 it follows that in recent years, at all age periods, replacement heifers had a live weight exceeding the breed standard, which is 250 kg at 10-month-old, 290 kg at 12-month-old and 390 kg at 18-month-old age. At the same time, an increase in the average live weight of the replacement livestock is observed every year. So, in 2020, the value of the indicator was 278 kg at the age of 10 months, 331 kg at the age of 12 months, 463 kg at the age of 18 months, which is 16 kg, 54 kg and 75 kg, or 6.1%, 19, 5% and 19.3%, more than in 2016, respectively. Thus, the analyzed period allows us to conclude about the effectiveness of zootechnical work to improve the rearing of replacement heifers on the farm, but it must be continued in order to reduce the age of first insemination to 15–17 months with the optimal live weight of the livestock.

Study of the efficiency of using biogenic metals for feeding calves

Breeding young cattle is important, especially during the transition period from dairy feeding to concentrated dry feed. The paper presents the results of the use of biogenic metal nicotinates for weaning calves to improve metabolism in animals. The aim of research. To investigate the effect of biogenic metal nicotinates: Zn, Cu, Fe, Co, Mn on the biochemical parameters of blood in calves after weaning. Materials and methods. The study was carried out during 2021 in the conditions of LLC "Agrofirma Lan", Sumy region, Sumy district, Kindrativka, Ukraine for breeding young cattle. The calves of the experimental group were given compound feed and a premix of nicotinates of biogenic metals: Zn, Cu, Fe, Co, Mn, manufactured by PPronos Agro" (1 g per 1 kg of feed). In the control group, combined feed and a premix with metal sulfates were used for 30 days. Results. An increase in the level of total protein in the body of calves of the experimental groups was established by 16.12 % in comparison with the control (p≤0.05). Also, in experimental animals, the activity of the enzymes alanine aminotransferase and aspartate aminotransferase was higher than the physiological norm, which shows an insignificant effect of nicotinates of biogenic metals on internal organs and systems. In the experimental group of calves, the level of magnesium was probably higher by 52.38 % and potassium – by 14.94 % compared to the control group (p≤0.05). It was found that the animals of the experimental groups probably had more zinc by 34.96 %; copper – by 35.72 %; iron – by 92.29 %; manganese – by 41.13 %; selenium – by 3.22 % and cobalt – by 98.33 % compared to the control (p≤0.05). Conclusions. The positive effect of the use of biogenic metal nicotinates on the metabolism of calves at weaning has been proven. It was found that the level of total protein in the body of calves of the experimental groups was probably higher by 16.12 %, magnesium - by 52.38 %; potassium - by 14.94 %. When determining the content of inorganic substances, it was found that the animals of the experimental groups probably had more zinc by 34.96 %; copper – by 35.72 %; iron – by 92.29 %; manganese – by 41.13 %; selenium – by 3.22 % and cobalt – by 98.33 % compared to the control (p≤0.05)

Enhanced Pathogenesis Caused by Influenza D Virus and Mycoplasma bovis Coinfection in Calves: a Disease Severity Linked with Overexpression of IFN-γ as a Key Player of the Enhanced Innate Immune Response in Lungs

Bovine respiratory disease (BRD) is among the most prevalent diseases in young cattle. BRD is due to complex interactions between viruses and/or bacteria, most of which have a moderate individual pathogenicity.

INFLUENCE OF DIFFERENT DOSES OF TISSUE BIOSTIMULANT ON BIOCHEMICAL BLOOD INDICES OF YOUNG CATTLE

Изучено влияние введения разных доз тканевого биостимулятора на биохимические показатели сыворотки крови ремонтного молодняка крупного рогатого скота. Опыт проводился в Алтайском крае на 4 группах ремонтных телочек Приобского типа черно-пестрой породы по 10 голов в каждой. При подборе животных учитывались возраст (1 мес) и живая масса (51,3±1,48 кг). Продолжительность опыта составляла 14 дней. Животным контрольной группы подкожно однократно вводили физиологический раствор в дозе 3,0 мл на 1 голову, I опытной — тканевый биостимулятор в дозе 2,0 мл, II — 3,0 мл, III — 4,0 мл на 1 голову. Опытную партию тканевого биостимулятора изготовили из субпродуктов и боенских отходов пантовых оленей по запатентованной технологии. Материалом для приготовления препарата служили мезентеральные лимфоузлы и средостения, селезенка, печень, матки с плодами (2—3 мес), плацента, отобранные в асептических условиях во время убоя здоровых животных. Введение тканевого биостимулятора телочкам в разных дозах способствовало повышению некоторых исследуемых биохимических показателей сыворотки крови. Наиболее оптимальной дозой применения тканевого биостимулятора следует считать 3,0 мл/гол, что способствует повышению содержания общего белка в сыворотке крови на 1,4% (P≤0,05), глюкозы — на 22,6% (P≤0,05) и снижению содержания холестерина на 12,3% (P≤0,05). The effect of the administration of different doses of the tissue biostimulant on the biochemical blood serum indices of replacement young cattle was studied. The experiment was carried out in the Altai Region in 4 groups of 10 Black-Pied replacement heifers of the Priobskiy type. When selecting the animals, their age (1 month) and live weight (51.3±1.48 kg) was taken into account. The experiment lasted 14 days. Saline solution was injected under the skin to the animals of the control group at a dose of 3.0 mL per 1 head; the tissue bio-stimulant was administered in the following doses: in the 1st trial group — 2.0 mL per head; the 2nd trial group 3.0 mL per head; the 3rd trial group — 4.0 mL per 1 head. The trial batch of the tissue bio-stimulant was made from velvet antler deer by-products and slaughterhouse offal by using the patent-protected technology. The tissue biostimulant was made from mesenteric lymph nodes and mediastinums, spleen, liver, uteri with 2—3 month old fetuses, and placentae collected under aseptic conditions from healthy animals at slaughter. The administration of the tissue bio-stimulant to heifers in different doses increased some of the studied biochemical blood serum indices. The tissue biostimulant dose of 3.0 mL per head should be considered the most optimal one; it increased the total protein content in blood serum by 1.4% (P≤0.05), glucose — by 22.6% (P≤0.05), and decreased cholesterol content by 12.3% (P≤0.05).

REQUIREMENTS FOR CREATING CONDITIONS FOR KEEPING ANIMALS ON DAIRY FARMS

The purpose of the research is – to develop requirements for creating comfortable conditions for keeping farm animals, taking into account EU standards, during milk production on farms. Research methods. During the development of requirements for the creation of comfortable conditions for keeping farm animals, taking into account EU standards, in the process of milk production on farms used the following basic regulations: Council Directive 98/58/EU, Council Directive 91/629/EU, Council Directive 92/46/EU, Commission Directive 89/362/EC. Requirements in the field of milk production, adapted to EU standards, are systematized by the following components: keeping, feeding, watering animals, milking cows, milk cooling, manure removal, creating a microclimate, veterinary care. Research results. In practice, during milk production it is necessary to create free, comfortable conditions for keeping cattle. Convenient access of service personnel to the place of calving of cows should be provided. In the conditions of the maternity ward, the technological areas should be 8-10 m² per cow. Young cattle, including calves, should be provided with a clean and dry rest area, protected from drafts. According to EU regulations, calves up to eight weeks of age can be kept in individual cages or in group cages. According to EU Council Directive 97/2, calves older than 8 weeks of age are kept only in group cages. In accordance with the EU Directive 97/2 technological areas for keeping young cattle with a live weight of up to 150 kg - must be at least 1,5 m² / head., Live weight 150 - 220 kg – 1,7 m² / head., Live weight 220 kg and more – 1,8 m² / goal. Technological areas for keeping animals with a live weight of 200 to 500 kg - from 2,7 to 4,7 m² / goal. The feeding front for young cattle with a live weight of 200 to 500 kg is from 0,4 to 0,6 m / goal. The technological area in the section per cow must be at least 6 m² / head. Rational sizes of boxes for rest of cows: width 1,2 m, length (near a wall) - 2,6 m, length (in paired boxes) - 2,45 m, height of a protection of a box - 1,1 ± 0,05 m, an inclination boxing floor towards the manure passage 5 ± 1%, the height of the boxing floor above the level of the manure passage 0,2 – 0,25 m. The width of the rest area of cows must be at least 6 m. In accordance with EU Council Directive 98/58 must be provided free access of animals to feeders and feed. EU Commission Decision 97/182 states that priority should be given to the feeding of whole milk substitutes when feeding calves. A feeding front for cows must be provided – 0,7 ± 0,05 m. In accordance with EU Council Directive 98/58, free access of animals to drinking troughs and water must be ensured. According to European standards, the total bacterial contamination of milk should be - ≤ 300 thousand KUO / cm³, the number of somatic cells in milk - ≤ 400 thousand /cm³. Drainage of wastewater from milking parlors, household premises of the farm is carried out using a separate from the manure removal system - sewer system. The bactericidal phase of fresh milk is 4 hours, no later than this period you need to start processing milk into dairy products. The depth of the manure channel in the livestock building should be 8 - 20 cm, the sides of which are located at right angles to the surface of the channel. The minimum width of the manure passage for cows should be 2,7 m. An acceptable level of air temperature for cows during the year from minus 10 °C to + 25 °C at a relative humidity of up to 80 %. Periodically carry out preventive veterinary measures, weighing animals, trimming the hooves of limbs in cows 2-4 times a year, monthly examination of cows for mastitis using mastitis detectors. Conclusions. For the first time in Ukraine, requirements have been developed to create comfortable conditions for keeping farm animals, taking into account EU standards, during milk production on farms. Requirements adapted to EU standards in the field of milk production are systematized by the following components: keeping, feeding, watering dairy cattle, milking cows, cooling milk, manure removal, creating a microclimate, veterinary care.

Metabolism in calves two weeks before diarrhea at the age of 19-52 days of life

The article presents data on the study of metabolism in calves two weeks before diarrhea at the age of 19-52 days of life. For this purpose, three groups of conditionally healthy animals of the Holstein breed were formed at the age of 19, 30, 52 days of life. To assess the metabolic status of young cattle, biochemical and general clinical blood tests were carried out. In the course of a laboratory blood test in young cattle, two weeks before disease manifestation, disorders of mineral and protein metabolism, a decrease in liver function were established. At the same time, with the increase in the age of the calves, the imbalance in protein metabolism was aggravated against the background of a change in diet and mineral metabolism improved as a result of the maturation of the digestive system.

Monitoring of the bovine digestive diseases on the Transbaikal territory

The dynamics of digestive diseases of bacterial etiology among cattle are presented. Statistical, laboratory and annual average data on registered animals with this pathology, mortality and forced slaughter of livestock were analysed for the period 2016-2020. It has been found that digestive diseases of bacterial etiology are prevalent both in young cattle (53.7% to 61.4%) and in adult cattle (43.1% of the total number of diseased animals). The following pathogens were identified on the basis of bacterial tests on biological material: enteropathogenic E. coli (37.8% of the total number of diseased animals) and group D streptococci, E. Faecalis (15.1%). The rest of the herd had associated infections (19.2%) and non-contagious digestive diseases (27.9%). As a result of this monitoring, a high incidence of digestive diseases among cattle in livestock farms in the following districts of the Trans-Baikal Territory was recorded: Akshinsky, Krasnochikoysky, Kyrinsky, Nerchinsky, Olovyaninsky, Priargunsky, Khiloksky, Shelopuginsky, Aginsky. The lowest prevalence of cattle diseases in organizations of various forms of ownership has been recorded in peasant farms in the region (6.8-10.9%). The average number of registered animals with this pathology is recorded in agricultural organizations (26.9-37.6%). A high percentage of these diseases were recorded in households (62.8%). When farms follow the organization of therapeutic and prophylactic measures, housing and feeding hygiene rules, it is possible to ensure the safety and productivity of livestock, high quality and safety of livestock products and to prevent bacterial diseases of the digestive organs.

Design and study of immunogenicity of virus vaccine against viral pneumoenteritis of calves

The immunogenicity of a virus vaccine against viral pneumoenteritis in young cattle was studied in laboratory animals. The results of the selection of optimal vaccine strains to study the effects of different inactivants on infectious rhinotracheitis virus, viral diarrhea, parainfluenza-3, respiratory syncytial virus, rotavirus and coronavirus are presented. The accumulation of avirulent vaccine virus strains was carried out using established virological methods on transplanted cell cultures of MDBC (calf kidney cells) and SPEV (fetal pig kidney cells). Theotropine and formalin were used as inactivating agents to develop inactivation regimes for vaccine strains - components of the experimental vaccine. The antigenic activity of attenuated strains of infectious rhinotracheitis virus, viral diarrhea, parainfluenza-3, respiratory syncytial virus, rotavirus and coronavirus on white mice and calves and the level of specific antibodies in the sera of guinea pigs immunized with vaccines using different adjuvants were studied. Avirulent strains of viruses were used in the design of a new virus vaccine against viral pneumoenteritis: infectious rhinotracheitis (IBR-VBF-VHAVM No. 404); diarrhea (VDVBF-VHAVM No. 406); parainfluenza-3 (PG-VBF-VHAVM No. 403); respiratory syncytial virus (RSV-VBF-VHAVM No. 405); rotavirus (RTV-VBF-VHAVM No. 401) and coronavirus (CV-VBFVHAVM No. 407). The selected vaccine strains are non-reactive and induce active production of antiviral antibodies in sufficiently high titres in both laboratory animals (white mice) and farm animals (cattle). The most effective inactivating agents are 0.1% theotropine and 0.2% formalin. Two types of oil-based adjuvants, ISA 15 and ISA 25, were used to select the optimal adjuvants for the design of the virus vaccine. Adjuvant ISA 15 was used at 15% of the antigen quantity, ISA 25 at 25%. The adjuvant IZA 15 at a concentration of 15% is the optimum adjuvant for the preparation of an experimental virus vaccine against viral pneumoenteritis in young cattle.

Influence of complex feed additives on the meat productivity of young cattle in the conditions of Yakutia

The paper presents the results of fattening young cattle in the conditions of Yakutia with the use of complex feed additives from local natural raw materials in their diets. One of the ways to increase the meat productivity of livestock is to optimize feeding by inserting complex feed additives into their diets. Therefore, studies were conducted to determine the effectiveness of complex feed additives in the fattening of young cattle in the conditions of Yakutia. At the beginning of the experiment, the live weight of the animals in the groups did not differ much, but starting from the age of 12 months, the superiority of the animals from the experimental groups is noted. In the period of 12-15 months of age, the animals from the experimental groups exceeded the growth rate of the control group by 6.70% and 4.76%. In the period of 15-18 months old, the animals of the control group were inferior to the experimental groups by 28.31% and 16.54%. On average, the animals of the experimental groups grew more intensively in comparison with the control group by 12.73% and 8.66 %. Analysis of animal slaughter data showed that the experimental groups were superior to the control group in a number of indicators, such as the mass of the steam carcass by 8.04% and 4.43 %, the mass of visceral fat by 18.59% and 7.39%. The organoleptic evaluation of meat products also confirmed the superiority of the experimental groups over the control group. Thus, the use of complex feed additives in the feeding of young cattle on fattening showed its effectiveness.