scholarly journals STUDIES ON THE MECHANISM OF ACTION OF RILEY VIRUS

1967 ◽  
Vol 125 (2) ◽  
pp. 277-288 ◽  
Author(s):  
B. W. J. Mahy ◽  
K. E. K. Rowson ◽  
C. W. Parr
Keyword(s):  
Enzyme Activity ◽  
Virus Infection ◽  
Mechanism Of Action ◽  
Clearance Rate ◽  
Plasma Clearance ◽  
Alanine Transaminase ◽  
Phosphoglucose Isomerase ◽  
Slow Phase ◽  
Rabbit Muscle ◽  
Blocking Agents

The plasma clearance of intravenously injected rabbit muscle LDH was studied. In normal mice the clearance followed a biphasic exponential curve comprising an initial fast and subsequent slow phase. Riley virus-infected mice showed only the slow phase of enzyme clearance. The change from fast to slow clearance rate in normal mice appeared to depend upon the level of plasma enzyme activity rather than on the amount of enzyme cleared. Treatment of mice with RES-blocking agents (cholesterol oleate and carbon) inhibited the fast clearance phase, whereas an RES-stimulating agent (stilbestrol) caused an accelerated rate of enzyme clearance. Riley virus infection was found to inhibit the clearance of phosphoglucose isomerase, but had no effect on the clearance of alanine transaminase. The activity of the former enzyme is raised in the plasma of infected mice, whereas the activity of the latter enzyme is unaltered.

Rat Plasma Clearance Rate and Organ Distribution of β-Hexosaminidase Isoenzymes from Human Serum

1992 ◽  
Vol 38 (9) ◽  
pp. 1893-1898 ◽  
Author(s):  
A Isaksson ◽  
B Hultberg ◽  
T Jonung
Keyword(s):  
Enzyme Activity ◽  
Liver Disease ◽  
Human Serum ◽  
Enzyme Immunoassay ◽  
Clearance Rate ◽  
Plasma Clearance ◽  
Chronic Alcoholism ◽  
Rat Plasma ◽  
Organ Distribution ◽  
Rapid Clearance

Abstract beta-Hexosaminidase (EC 3.2.1.30) is markedly increased in human serum in liver disease, chronic alcoholism, and pregnancy. Knowledge of the clearance rate of plasma/serum beta-hexosaminidase is necessary to evaluate this increase. We studied the plasma clearance of beta-hexosaminidase isoenzymes (purified from human serum and placenta) after their infusion into rat circulation. A recently developed enzyme immunoassay method was used to measure the human beta-hexosaminidase isoenzymes; this method relies on both immunoreactivity and enzyme activity, so intact human isoenzymes were measured. In comparison with the placental isoenzymes (t1/2 less than 2 min), the serum forms showed a considerably slower clearance (t1/2 = 2-4 h). However, desialylation of the serum forms resulted in their rapid clearance (t1/2 less than 2 min). The organ localization of the enzyme eliminated from the circulation was investigated 24 h after infusion. Placental and native serum isoenzymes accumulated mainly in the liver and the spleen. Desialylated serum forms were almost exclusively localized to the liver.

Catabolism of Human Fibrinogen Fragment D in Normal Subjects and Patients with Liver Cirrhosis

1980 ◽  
Vol 44 (03) ◽  
pp. 146-149 ◽  
Author(s):  
Nicole Ardaillou ◽  
Jeannine Yvart ◽  
Philippe Le Bras ◽  
Marie-José Larrieu
Keyword(s):  
Liver Cirrhosis ◽  
Clearance Rate ◽  
Plasma Clearance ◽  
Normal Subjects ◽  
Fractional Catabolic Rate ◽  
Human Fibrinogen ◽  
Plasma Pool ◽  
Catabolic Rate ◽  
Chloramine T

SummaryThe catabolism of human fragment D, (FgD), obtained by plasmin digestion of fibrinogen has been investigated in normal subjects and patients with liver cirrhosis and the results compared with those obtained for fibrinogen (Fg). Fg was labelled with I-125 and Fg D with I-131 using the chloramine T method. The plasma disappearance curves of both labelled proteins fitted a two exponential curve. In controls the plasma clearance rate of Fg D was greater than that of Fg as shown by the marked difference between the half-lives of these two tracers: 8,9 and 83,5 hours for Fg D and Fg respectively. The fractional catabolic rate of Fg D was 3.38 times the plasma pool per day. In nine patients with liver cirrhosis, catabolism of Fg was not modified. In contrast, catabolism of Fg D was significantly reduced with a half life of 13.0 hours and a low fractional catabolic rate. These results suggest the role of the liver in the catabolism of Fg D in man.

scholarly journals Mechanism of Action of Rabbit Muscle Phosphoglucomutase

1974 ◽  
Vol 249 (10) ◽  
pp. 3166-3169 ◽  
Author(s):  
Olov Wålinder ◽  
Jayant G. Joshi
Keyword(s):  
Mechanism Of Action ◽  
Rabbit Muscle

scholarly journals Pseudoisoenzymes of Rabbit Muscle Phosphoglucose Isomerase

1972 ◽  
Vol 247 (4) ◽  
pp. 1170-1179
Author(s):  
Michael N. Blackburn ◽  
John M. Chirgwin ◽  
Gordon T. James ◽  
Thomas D. Kempe ◽  
Thomas F. Parsons ◽  
...  
Keyword(s):  
Phosphoglucose Isomerase ◽  
Rabbit Muscle

Studies on the Sympathetic Control of Normal and Diseased Synovial Blood Vessels: The Effect of α and β Receptor Stimulation and Inhibition, Monitored by the 133Xenon Clearance Technique

1971 ◽  
Vol 40 (2) ◽  
pp. 197-209 ◽  
Author(s):  
W. C. Dick ◽  
R. Jubb ◽  
W. W. Buchanan ◽  
J. Williamson ◽  
K. Whaley ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Clearance Rate ◽  
Qualitative Difference ◽  
Normal Subjects ◽  
Indirect Measure ◽  
Sympathetic Control ◽  
Blocking Agents ◽  
Disappearance Curve ◽  
Β Receptor ◽  
T Values

1. The half-life (T½) of the disappearance curve of 133Xe from the knee joint has been used as an indirect measure of synovial perfusion. 2. The effects of intra-articularly administered isoprenaline and noradrenaline and their respective blocking agents, propranolol and phentolamine, on the T½ values have been investigated in normal subjects and in patients with osteoarthritis and rheumatoid arthritis. 3. In normal subjects isoprenaline increased the clearance rate and this effect was blocked by propranolol. Noradrenaline decreased the clearance rate and this effect was blocked by phentolamine. 4. The injection of phentolamine in normal subjects caused an increase in synovial perfusion, suggesting the presence of a basal α-constrictor tone. 5. In the patients with osteoarthritis and rheumatoid arthritis quantitative differences were found in the responses to isoprenaline and noradrenaline and their respective blocking agents. 6. There appeared to be a qualitative difference in the case of phentolamine; it is possible that vasoconstrictor tone may be lost in inflamed joints.

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