scholarly journals Persistence of chronic myelocytic leukemia despite deletion of rearranged bcr/c-abl sequences in blast crisis.

1986 ◽  
Vol 164 (5) ◽  
pp. 1389-1396 ◽  
Author(s):  
C R Bartram ◽  
J W Janssen ◽  
R Becher ◽  
A de Klein ◽  
G Grosveld
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
Southern Blot ◽  
Blast Crisis ◽  
Cell Lineage ◽  
Chronic Myelocytic Leukemia ◽  
Northern Blots ◽  
Myelocytic Leukemia ◽  
Ph Chromosome

We report on a Ph+ chronic myelocytic leukemia (CML) case, cytogenetically characterized by the occurrence of a second Philadelphia (Ph) chromosome in lymphoid blast crisis of T cell lineage. In situ hybridization analyses showed a deletion of translocated c-abl sequences, present on the Ph during chronic state, from both Ph in acute state. Moreover, Southern blot analyses of blastic cells exhibited a rearrangement within bcr, but a deletion of 5' bcr sequences, and Northern blots failed to detect the hybrid 8.5 kb bcr/c-abl transcript usually observed in Ph+ CML.

Some observations on fluorescence in situ hybridization evaluation of chronic myelocytic leukemia

1997 ◽  
Vol 98 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Zhong Chen ◽  
Mathilda Notohamiprodjo ◽  
Paul D. Richards ◽  
Frank B. Lane ◽  
Rodman Morgan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

scholarly journals Localization of the human c-sis oncogene in Ph1-positive and Ph1- negative chronic myelocytic leukemia by in situ hybridization

Blood ◽  
1984 ◽  
Vol 63 (1) ◽  
pp. 223-225 ◽  
Author(s):  
CR Bartram ◽  
A de Klein ◽  
A Hagemeijer ◽  
G Grosveld ◽  
N Heisterkamp ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Region ◽  
Philadelphia Chromosome ◽  
Active Role ◽  
Chromosome 22 ◽  
Chronic Myelocytic Leukemia ◽  
Metaphase Chromosomes ◽  
Myelocytic Leukemia ◽  
Negative Case

Abstract Oncogenes are a group of evolutionary conserved cellular genes (c-onc) homologous to the transforming genes of oncogenic retroviruses (v-onc). Some of them are localized near the breakpoints of specific chromosomal aberrations occurring in various neoplasms, as for example the Philadelphia translocation, t(9;22)(q34;q11), in chronic myelocytic leukemia (CML). Recently, we localized the human c-abl oncogene to chromosome region 9q34 and demonstrated a translocation of this gene to the Philadelphia chromosome (Ph1,22q-) in various forms of Ph1- positive, but not Ph1-negative, chronic myelocytic leukemia (CML). Another human oncogene, c-sis, is located on chromosome 22 and was recently reported to be transferred to chromosome 9q+ in one CML patient. We have now studied 2 CML patients with classic and variant types of Ph1 translocation, one Ph1-negative case, and a healthy control using in situ hybridization of a c-sis probe to metaphase chromosomes. These studies show that c-sis: (1) is localized to region 22q12.3-q13.1, far away from the breakpoint region 22q11 in CML, (2) segregates with the translocated part of chromosome 22 to different chromosomes in Ph1-positive patients, and (3) remains on chromosome 22 in the Ph1-negative case. Therefore, these data give no support for an active role of the c-sis gene in the generation of CML. Thus, if either of these two oncogenes is involved in the development of Ph1-positive CML, c-abl appears to be the more important one.

scholarly journals Localization of the human c-sis oncogene in Ph1-positive and Ph1- negative chronic myelocytic leukemia by in situ hybridization

Blood ◽  
1984 ◽  
Vol 63 (1) ◽  
pp. 223-225 ◽  
Author(s):  
CR Bartram ◽  
A de Klein ◽  
A Hagemeijer ◽  
G Grosveld ◽  
N Heisterkamp ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Region ◽  
Philadelphia Chromosome ◽  
Active Role ◽  
Chromosome 22 ◽  
Chronic Myelocytic Leukemia ◽  
Metaphase Chromosomes ◽  
Myelocytic Leukemia ◽  
Negative Case

Oncogenes are a group of evolutionary conserved cellular genes (c-onc) homologous to the transforming genes of oncogenic retroviruses (v-onc). Some of them are localized near the breakpoints of specific chromosomal aberrations occurring in various neoplasms, as for example the Philadelphia translocation, t(9;22)(q34;q11), in chronic myelocytic leukemia (CML). Recently, we localized the human c-abl oncogene to chromosome region 9q34 and demonstrated a translocation of this gene to the Philadelphia chromosome (Ph1,22q-) in various forms of Ph1- positive, but not Ph1-negative, chronic myelocytic leukemia (CML). Another human oncogene, c-sis, is located on chromosome 22 and was recently reported to be transferred to chromosome 9q+ in one CML patient. We have now studied 2 CML patients with classic and variant types of Ph1 translocation, one Ph1-negative case, and a healthy control using in situ hybridization of a c-sis probe to metaphase chromosomes. These studies show that c-sis: (1) is localized to region 22q12.3-q13.1, far away from the breakpoint region 22q11 in CML, (2) segregates with the translocated part of chromosome 22 to different chromosomes in Ph1-positive patients, and (3) remains on chromosome 22 in the Ph1-negative case. Therefore, these data give no support for an active role of the c-sis gene in the generation of CML. Thus, if either of these two oncogenes is involved in the development of Ph1-positive CML, c-abl appears to be the more important one.

scholarly journals Extranodal NK/T-cell lymphoma in Tunisia: clinicopathological features, immunophenotype and EBV infection

2019 ◽  
Vol 31 (1) ◽  
Author(s):  
Nabiha Missaoui ◽  
Sarra Mestiri ◽  
Aida Bouriga ◽  
Nihed Abdessayed ◽  
Mouna Belakhdher ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
Granzyme B ◽  
Clinicopathological Features ◽  
Histopathological Diagnosis ◽  
Ebv Infection ◽  
Nk T Cell ◽  
Epstein Barr ◽  
Hodgkin's Lymphomas

Abstract Background Extranodal NK/T-cell lymphomas (ENKTL) are rare non-Hodgkin’s lymphomas with aggressive clinical behavior. ENKTL are frequently associated with the Epstein-Barr virus (EBV). Data on ENKTL in Africa and Arab world are extremely limited. The study investigated the clinicopathological characteristics, EBV infection, and immunophenotype of ENKTL in Tunisia. We conducted a retrospective study of ENKTL. Main clinicopathological features were reported. The expression of CD3, CD4, CD5, CD8, CD20, CD56, CD57, and Granzyme B were analyzed by immunohistochemistry. EBV infection was detected by IHC (LMP-1) and Epstein-Barr encoding region (EBER1/2) in situ hybridization. Results A total of nine ENKTL were identified (mean age of 48 years and male-to-female ratio of 8:1). There were five nasal ENKTL, and the remaining four cases had extranasal involvement (palate, sub-mandibular gland, skin, and soft tissues of the ankle). The histopathology showed a lymphoid and pleomorphic proliferation characterized by images of angiocentrism. Strong and diffuse CD3 expression was observed in all cases. Tumor cells exhibited an expression of CD5 (two cases), CD8 (three cases), CD56 (six cases), CD57 (three cases), and Granzyme B (eight cases). All ENKTL cases were EBV-associated. Overall 5-year survival rate was 57%. Although six ENKTL were diagnosed at early clinical stages, the prognosis was unfavorable and associated with patient death in three cases. Conclusions ENKTL are exceptional in Tunisia with unfavorable outcome. Histopathological diagnosis remains challenging in clinical practice. However, a careful histopathological examination combined with a correct interpretation of immunohistochemistry and in situ hybridization results refines the ENKTL diagnosis.

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