Assessment of the effects of four crosslinking agents on gelatin hydrogel for myocardial tissue engineering applications

ABSTRACTThe objective of the present in vitro research was to determine cardiomyocyte function on poly lactic-co-glycolic acid (50:50 (PLA:PGA); PLGA) with greater amounts of carbon nanofibers (CNFs) and variations in CNF size, for myocardial tissue engineering applications. The addition of CNFs would increase conductivity and strength of pure PLGA. For this reason, different PLGA: CNF ratios (100:0, 75:25, 50:50, 25:75, 0:100 wt.%) were created and conductivity and cytocompatibility properties with human cardiomyocytes were determined. Results showed that PLGA:CNF materials were conductive and that conductivity increased with greater amounts of PLGA added, from 0 S.m-1 for 100:0 wt.% (pure PLGA) to 5.5x10-3 S.m-1 for 0:100 wt.% (pure CNFs) material. Furthermore, results indicated that cardiomyocyte density increased with greater amounts of CNFs of 200nm in diameter in PLGA (up to 25:75 wt.% , PLGA:CNFs). This study, thus, provided an alternative conductive scaffold using nanotechnology which should be further explored for cardiovascular applications.

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Development of a three dimensional (3D) knitted scaffold for myocardial tissue engineering. Part II: biological performance of the knitted scaffolds

Journal of the Textile Institute ◽

10.1080/00405000.2021.1907990 ◽

2021 ◽

pp. 1-11

Author(s):

Derya Haroglu ◽

Ahmet Eken ◽

Zeynep Burçin Gönen ◽

Dilek Bahar

Keyword(s):

Tissue Engineering ◽

Three Dimensional ◽

Myocardial Tissue ◽

Biological Performance ◽

Myocardial Tissue Engineering

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Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Polymers ◽

10.3390/polym13050794 ◽

2021 ◽

Vol 13 (5) ◽

pp. 794 ◽

Cited By ~ 1

Author(s):

Su Jeong Lee ◽

Ji Min Seok ◽

Jun Hee Lee ◽

Jaejong Lee ◽

Wan Doo Kim ◽

...

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Sodium Alginate ◽

Three Dimensional ◽

Fibroblast Cell ◽

Chemical Crosslinking ◽

Fibroblast Cell Line ◽

Engineering Applications ◽

Encapsulated Cells ◽

Crosslinking Agents

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

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