Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

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Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications

Journal of Functional Biomaterials ◽

10.3390/jfb12030045 ◽

2021 ◽

Vol 12 (3) ◽

pp. 45

Author(s):

Yasaman Delkash ◽

Maxence Gouin ◽

Tanguy Rimbeault ◽

Fatemeh Mohabatpour ◽

Petros Papagerakis ◽

...

Keyword(s):

Tissue Engineering ◽

Sodium Alginate ◽

Vascular Endothelial Cells ◽

Three Dimensional ◽

Heart Tissue ◽

Biological Properties ◽

3D Bioprinting ◽

Engineering Applications ◽

In Vitro Characterization

Three-dimensional (3D) bioprinting is an emerging fabrication technique to create 3D constructs with living cells. Notably, bioprinting bioinks are limited due to the mechanical weakness of natural biomaterials and the low bioactivity of synthetic peers. This paper presents the development of a natural bioink from chicken eggwhite and sodium alginate for bioprinting cell-laden patches to be used in endothelialized tissue engineering applications. Eggwhite was utilized for enhanced biological properties, while sodium alginate was used to improve bioink printability. The rheological properties of bioinks with varying amounts of sodium alginate were examined with the results illustrating that 2.0–3.0% (w/v) sodium alginate was suitable for printing patch constructs. The printed patches were then characterized mechanically and biologically, and the results showed that the printed patches exhibited elastic moduli close to that of natural heart tissue (20–27 kPa) and more than 94% of the vascular endothelial cells survived in the examination period of one week post 3D bioprinting. Our research also illustrated the printed patches appropriate water uptake ability (>1800%).

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Rheological Properties of İnjectable Hyaluronic Acid Hydrogels for Soft Tissue Engineering Applications

Biointerface Research in Applied Chemistry ◽

10.33263/briac111.84248430 ◽

2020 ◽

Vol 11 (1) ◽

pp. 8424-8430

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Soft Tissue ◽

Rheological Properties ◽

Three Dimensional ◽

Diglycidyl Ether ◽

Potential Candidate ◽

Engineering Applications ◽

Crosslinker Concentration ◽

Soft Tissue Engineering

Hydrogels are cross-linked three-dimensional (3D) polymeric network, which can hold the water within its porous structure. They have recently been used in various biomedical applications. In this study, injectable hyaluronic acid (HA) hydrogels were prepared using different concentrations of 1,4-butanediol diglycidyl ether (BDDE) as a crosslinker (1-5% w/w) and investigated their rheological, swelling and injectability properties. The results demonstrated that the rheological characteristics of hydrogels enhanced with increasing crosslinker concentration. The elastic modulus of the hydrogels ranged from 280 Pa to 990 Pa, while the complex viscosities were found between 42 Pa.s and 190 Pa.s at an oscillation frequency of 1 Hz. These results clearly suggest that the injectable HA hydrogels are a potential candidate for various soft tissue engineering applications due to their highly tunable rheological properties.

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Injectable hydrogel-based scaffolds for tissue engineering applications

Reviews in Chemical Engineering ◽

10.1515/revce-2015-0074 ◽

2017 ◽

Vol 33 (1) ◽

Cited By ~ 18

Author(s):

Tanya Portnov ◽

Tiberiu R. Shulimzon ◽

Meital Zilberman

Keyword(s):

Tissue Engineering ◽

Structural Integrity ◽

Three Dimensional ◽

Dry Weight ◽

Polymer Chains ◽

Design Parameters ◽

Engineering Applications ◽

Encapsulated Cells ◽

Polymeric Networks ◽

Injectable Scaffolds

AbstractHydrogels are highly hydrated materials that may absorb from 10% to 20% up to hundreds of times their dry weight in water and are composed of three-dimensional hydrophilic polymeric networks that are similar to those in natural tissue. The structural integrity of hydrogels depends on cross-links formed between the polymer chains. Hydrogels have been extensively explored as injectable cell delivery systems, owing to their high tissue-like water content, ability to mimic extracellular matrix, homogeneously encapsulated cells, efficient mass transfer, amenability to chemical and physical modifications, and minimally invasive delivery. A variety of naturally and synthetically derived materials have been used to form injectable hydrogels for tissue engineering applications. The current review article focuses on these biomaterials, on the design parameters of injectable scaffolds, and on the

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Three-dimensional artificial liver platforms for drug screening and tissue engineering applications

10.32657/10356/142517 ◽

2020 ◽

Author(s):

◽

Gaia Ferracci

Keyword(s):

Tissue Engineering ◽

Drug Screening ◽

Three Dimensional ◽

Artificial Liver ◽

Engineering Applications

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Hyaluronic Acid-gelatin Fibrous Scaffold Produced by Electrospinning of Their Aqueous Solution for Tissue Engineering Applications

10.1557/proc-1140-hh06-19-dd03-19 ◽

2008 ◽

Author(s):

Ying Liu ◽

Richard A. Clark ◽

Lei Huang ◽

Miriam H. Rafailovich

Keyword(s):

Aqueous Solution ◽

Tissue Engineering ◽

Hyaluronic Acid ◽

Engineering Applications ◽

Fibrous Scaffold

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A Proposed Fabrication Method of Novel PCL-GEL-HAp Nanocomposite Scaffolds for Bone Tissue Engineering Applications

Advanced Composites Letters ◽

10.1177/096369351001900401 ◽

2010 ◽

Vol 19 (4) ◽

pp. 096369351001900 ◽

Cited By ~ 22

Author(s):

A. Hamlekhan ◽

M. Mozafari ◽

N. Nezafati ◽

M. Azami ◽

H. Hadipour

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Mechanical Test ◽

Fibroblast Cell ◽

Mouse Fibroblast ◽

Fabrication Method ◽

Engineering Applications ◽

Poly Caprolactone

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

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