scholarly journals Influence of parity and live weight on the concentration of Pregnancy-Specific Protein B (PSPB) in Kedah Kelantan (KK) cattle

2021 ◽  
Vol 756 (1) ◽  
pp. 012019
Author(s):  
N. A. Hanafiah ◽  
R. A. R. R. Khalif ◽  
N. D. Rusli ◽  
C. H. Hasnita ◽  
M. M. Rahman ◽  
...  
Keyword(s):  
Live Weight ◽  
Specific Protein ◽  
Protein B

Secretion of progesterone, estradiol-17β, PGE, PGF2α, and pregnancy-specific protein B by 90-day intact and ovariectomized pregnant ewes☆

1999 ◽  
Vol 58 (2-4) ◽  
pp. 139-148 ◽  
Author(s):  
Y.S Weems ◽  
P.J Bridges ◽  
B.R LeaMaster ◽  
R.G Sasser ◽  
D.L Vincent ◽  
...  
Keyword(s):  
Specific Protein ◽  
Estradiol 17Β ◽  
Protein B

scholarly journals Detection of pregnancy in sheep using an ELISA for pregnancy-specific protein B

2018 ◽  
Vol 66 (2) ◽  
pp. 329-336
Author(s):  
Tímea Milisits-Németh ◽  
Orsolya Gabriella Balogh ◽  
István Egerszegi ◽  
László Kern ◽  
R. Garth Sasser ◽  
...  
Keyword(s):  
Accurate Method ◽  
Specific Protein ◽  
Economic Benefits ◽  
Seasonal Breeding ◽  
Serum Samples ◽  
Mating Period ◽  
Blood Samples ◽  
Sheep Production ◽  
Protein B

The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals. Ninety-three ewes of three breeds (British Milksheep – BM, Lacaune – L and Transylvanian Racka – TR), each from three farms in Hungary, were included in the study. BM and L ewes were artificially inseminated (AI). Thirty-five days after AI, all ewes were examined by transabdominal ultrasound. The TR flock was mated naturally over a six-week period. At the end of the mating period, the ewes were similarly examined by ultrasound. Blood samples were taken from all pregnant ewes twice (35 and 65 days after AI), and serum samples were assayed by the BioPRYN test. It can be concluded that the detection of serum PSPB by ELISA is a much easier, safer, less expensive and highly accurate method for the detection of ovine pregnancy. Although some breed-related differences were detectable at 35 and 65 days post breeding, no differences in oPSPB levels were found in pregnant ewes carrying different numbers of fetuses.

scholarly journals Pregnancy-Specific Protein B in Yankasa Ewes During Pregnancy and Postpartum Periods

2021 ◽  
Vol 44 (1) ◽  
pp. 55-62
Author(s):  
Adewale Ayodeji Adeyeye ◽  
Yusha'u Usman Abubakar ◽  
Olufisayo Oluwadamilare Leigh ◽  
Iyorhembe Utim Ate ◽  
Jashilagari Stephen ◽  
...  
Keyword(s):  
Specific Protein ◽  
Trophoblast Cells ◽  
Blood Samples ◽  
Pregnancy And Postpartum ◽  
Group A ◽  
Group B ◽  
Protein B

Abstract Pregnancy-specific protein B (PSPB) is produced by mono and binucleate trophoblast cells in the placenta of ruminants during pregnancy. This study was designed to determine the pattern of serum PSPB in Yankasa ewes during pregnancy and postpartum periods. Mature cycling Yankasa ewes were synchronized and divided into two groups A (n=11) and B (n=13). Group A was bred, while group B was unbred. Blood samples for PSPB assessment were collected from the ewes starting from the day of breeding until 4 weeks post-lambing. All pregnant Yankasa ewes lambed with singleton lambs after an average of 151.18 days. There was a significant (p<0.05) increase in PSPB in pregnant compared with the non-pregnant ewes in the period between 3 weeks post-breeding and 3 weeks post-lambing. Peaks were detected in the first (100.60 ng/ml), second (133.90 ng/ml), and third (114.82 ng/ml) trimesters at 5, 10 and 21 weeks of gestation, respectively, but steadily decreased within 4 weeks (2.38 ng/ml) postpartum. In conclusion, PSPB detected pregnancy in Yankasa ewes from 3 weeks post-breeding with peak levels at 5, 10 and 21 weeks post-breeding in the first, second, and third trimesters, respectively. PSPB decreased gradually after lambing until 4 weeks postpartum.

Sign in / Sign up

Export Citation Format

Share Document