Roscovitine Treatment Improves Synchronization of Donor Cell Cycle in G0/G1 Stage and In Vitro Development of Handmade Cloned Buffalo (Bubalus bubalis) Embryos

2012 ◽  
Vol 14 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Mushariffa Muzaffer ◽  
G. Krishnakanth ◽  
Ambika P. Saha ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Donor Cell ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Vitro Development

Stage specific expression of cell cycle genes during in vivo or in vitro development of ovarian follicles in sheep

2016 ◽  
Vol 143 ◽  
pp. 1-7 ◽  
Author(s):  
V. Praveen Chakravarthi ◽  
S.S.R. Kona ◽  
A.V.N. Siva Kumar ◽  
M. Bhaskara ◽  
V.H. Rao
Keyword(s):  
Cell Cycle ◽  
Ovarian Follicles ◽  
Specific Expression ◽  
In Vitro Development ◽  
Cell Cycle Genes ◽  
Vitro Development

Expression of nitric oxide synthase isoforms in different stages of buffalo (Bubalus bubalis) ovarian follicles: Effect of nitric oxide on in vitro development of preantral follicle

2012 ◽  
Vol 77 (2) ◽  
pp. 280-291 ◽  
Author(s):  
Pawan K. Dubey ◽  
Vrajesh Tripathi ◽  
Ram Pratap Singh ◽  
G. Saikumar ◽  
Amar Nath ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Bubalus Bubalis ◽  
Ovarian Follicles ◽  
Preantral Follicle ◽  
In Vitro Development ◽  
Oxide Synthase ◽  
Vitro Development

Donor cell trichostatin A treatment improves the in vitro development of cloned goat embryos

2015 ◽  
Vol 124 ◽  
pp. 76-80 ◽  
Author(s):  
Yi Min Wang ◽  
Xiang Bin Ding ◽  
Xin Feng Liu ◽  
Yong Zhang
Keyword(s):  
Trichostatin A ◽  
Donor Cell ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Metabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfer

2017 ◽  
Author(s):  
◽  
Bethany Rae Mordhorst
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Donor Cell ◽  
Nuclear Reprogramming ◽  
In Vitro Development ◽  
Fetal Fibroblasts ◽  
Pharmaceutical Agents ◽  
Vitro Development

Gene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. This process involves using cells from a donor (which may have been gene edited) that are typically grown in culture and using their nuclear content to reconstruct a new zygote. To do this, the cell may be placed in the perivitelline space of an enucleated oocyte and activated artificially by a calcium-containing media and electrical pulse waves. While it is remarkable that this process works, it is highly inefficient. In pigs the success of transferred embryos becoming live born piglets is only 1-3%. The creation of more cloned pigs enables further study for the benefit of both A) biomedicine in the development of prognosis and treatments and B) agriculture, whether it be for disease resistance, feed efficiency, gas emissions, etc. Two decades of research has not drastically improved the cloning efficiency of most mammals. One of the main impediments to successful cloning is thought to be due to inefficient nuclear reprogramming and remodeling of the donor cell nucleus. In the following chapters we detail our efforts to improve nuclear reprogramming of porcine fetal fibroblasts by altering the metabolism to be more blastomere-like in nature. We used two methods to alter metabolism 1) pharmaceutical agents and 2) hypoxia. After treating donor cells both methods were used in nuclear transfer. Pharmaceutical agents did not improve in vitro development of gestational survival of clones. Hypoxia did improve in vitro development and we are currently awaiting results of gestation.

scholarly journals α-Tocopherol and l-ascorbic acid increase the in vitro development of IVM/IVF swamp buffalo (Bubalus bubalis) embryos

animal ◽  
2008 ◽  
Vol 2 (10) ◽  
pp. 1486-1490 ◽  
Author(s):  
K. Saikhun ◽  
T. Faisaikarm ◽  
Z. Ming ◽  
K.H. Lu ◽  
Y. Kitiyanant
Keyword(s):  
Ascorbic Acid ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Swamp Buffalo ◽  
Vitro Development

Effect of cryoprotectants and their concentration on in vitro development of vitrified-warmed immature oocytes in buffalo (Bubalus bubalis)

2004 ◽  
Vol 61 (5) ◽  
pp. 831-842 ◽  
Author(s):  
N.A Wani ◽  
S.N Maurya ◽  
A.K Misra ◽  
V.B Saxena ◽  
B.D Lakhchaura
Keyword(s):  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Immature Oocytes ◽  
Vitro Development

scholarly journals Supplementation of L-ascorbic acid improves the in vitro development of buffalo (Bubalus bubalis) embryos and alters the expression of apoptosis-related genes

2021 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Mayank Roshan ◽  
Diksha Dua ◽  
Ankur Sharma ◽  
Manish Tiwari ◽  
ManojKumar Singh ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Vitro Development

17 TREATMENT WITH MGCD 0103 IMPROVES THE IN VITRO DEVELOPMENT OF PORCINE EMBRYOS DERIVED FROM SOMATIC CELL NUCLEAR TRANSFER

2016 ◽  
Vol 28 (2) ◽  
pp. 138
Author(s):  
H.-Y. Zhu ◽  
L. Jin ◽  
Q. Guo ◽  
Y.-C. Zhang ◽  
X.-C. Li ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Donor Cell ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
In Vitro Development ◽  
Single Donor ◽  
Or Groups ◽  
Humidified Air ◽  
Vitro Development

We use MGCD 0103 to test whether the treatment with this novel histone deacetylase inhibitor improves the in vitro development of porcine somatic cell NT (SCNT) embryos. Matured eggs were cultured in medium supplemented with 0.05 M sucrose and 0.4 μg mL–1 demecolcine for 1 h. Treated eggs with a protruding membrane were transferred to medium supplemented with 5 μg mL–1 cytochalasin B and 0.4 μg mL–1 demecolcine. Protrusions were then removed by aspirating with a 15-μm inner diameter glass pipette. A single donor cell was inserted into the perivitelline space of each egg and electrically fused using 2 direct pulses of 150 V mm–1 for 50 μs in 0.28 M mannitol. Fused eggs cultured for 1 h were activated by 2 direct pulses of 100 V mm–1 for 20 μs and incubated with 2 mM 6-DMAP for 4 h. Subsequently, the cloned embryos were cultured in medium for 7 days at 38.5°C in 5% CO2 humidified air. In Experiment 1, after activation and treatment with 6-DMAP for 4 h, the SCNT embryos were cultured in medium supplemented with 0, 0.2, 2, or 20 μM MGCD 0103 for 24 h and then transferred to medium without MGCD 0103. In Experiment 2, SCNT embryos were cultured in medium supplemented with 0.2 μM MGCD 0103 for 0, 6, 24, or 48 h and then transferred to medium without MGCD 0103. As shown in Table 1, development to the blastocyst stage increased in SCNT embryos treated with 0.2 μM MGCD 0103 compared with the control or groups treated with 2 or 20 μM MGCD 0103 (25.51 v. 10.74, 3.53, 3.20%, respectively; P < 0.05). As shown in Table 1, treatment for 6 h with 0.2 μM MGCD 0103 significantly improved the rate of blastocyst formation compared with the control or groups treated for 24 or 48 h (21.17 v. 10.48, 19.23, 10.20%, respectively; P < 0.05). Our results suggested that 0.2 μM MGCD 0103 treatment for 6 h can improve in vitro developmental competence of porcine SCNT embryos. Table 1.In vitro development of pig SCNT embryos with different concentrations of MGCD 0103 for 24 h, and with 0.2 μM MGCD 0103 for different durations

scholarly journals Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level

2015 ◽  
Vol 17 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Himanshu Agrawal ◽  
Prabhat Palta ◽  
Manmohan S. Chauhan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Dna Methyltransferase ◽  
Bubalus Bubalis ◽  
Dna Methyltransferase 1 ◽  
In Vitro Development ◽  
Vitro Development ◽  
Methyltransferase 1
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