scholarly journals Rethinking Phage Ecology by Rooting it Within an Established Plant Framework

PHAGE ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 121-136
Author(s):  
Martha R.J. Clokie ◽  
Bob G. Blasdel ◽  
Benoit O.L. Demars ◽  
Thomas Sicheritz-Pontén
2021 ◽  
Author(s):  
M. T. Jahn ◽  
T. Lachnit ◽  
S. M. Markert ◽  
C. Stigloher ◽  
L. Pita ◽  
...  

AbstractBacteriophages (phages) are ubiquitous elements in nature, but their ecology and role in animals remains little understood. Sponges represent the oldest known extant animal-microbe symbiosis and are associated with dense and diverse microbial consortia. Here we investigate the tripartite interaction between phages, bacterial symbionts, and the sponge host. We combined imaging and bioinformatics to tackle important questions on who the phage hosts are and what the replication mode and spatial distribution within the animal is. This approach led to the discovery of distinct phage-microbe infection networks in sponge versus seawater microbiomes. A new correlative in situ imaging approach (‘PhageFISH-CLEM‘) localised phages within bacterial symbiont cells, but also within phagocytotically active sponge cells. We postulate that the phagocytosis of free virions by sponge cells modulates phage-bacteria ratios and ultimately controls infection dynamics. Prediction of phage replication strategies indicated a distinct pattern, where lysogeny dominates the sponge microbiome, likely fostered by sponge host-mediated virion clearance, while lysis dominates in seawater. Collectively, this work provides new insights into phage ecology within sponges, highlighting the importance of tripartite animal-phage-bacterium interplay in holobiont functioning. We anticipate that our imaging approach will be instrumental to further understanding of viral distribution and cellular association in animal hosts.


1975 ◽  
Vol 17 (4) ◽  
pp. 517-524 ◽  
Author(s):  
F. B. Holl

The problems of feeding a rapidly expanding world population with decreasing arable land resources are placing increasing demands on our genetic resources and the plant breeders who exploit them. Innovative or novel genetic approaches may provide solutions to some of the problems of genetic analysis and the development of additional genetic variability in plants. The present status of techniques such as wide crossing, DNA feeding, tissue, cell and protoplast culture and somatic cell hybridisation is discussed. Plant tissue culture procedures may enable us to expand the gene pools for disease and pest resistance, greater tolerance to environmental stress, increased quality or possibly to develop new plant types. However, the utility of innovative procedures will require a rigorous evaluation of their potential and limitations, and the ability to produce material which can be readily introgressed into established plant breeding systems.


2009 ◽  
pp. 251-280 ◽  
Author(s):  
T. Frede Thingstad ◽  
Gunnar Bratbak ◽  
Mikal Heldal
Keyword(s):  

2017 ◽  
Vol 20 (6) ◽  
pp. 1387-1402
Author(s):  
Sandra L. Hoffberg ◽  
Rodney Mauricio ◽  
Richard J. Hall

2018 ◽  
Vol 216 ◽  
pp. 141-149 ◽  
Author(s):  
M.A. Rossini ◽  
M.E. Otegui ◽  
E.L. Martínez ◽  
G.A. Maddonni

1994 ◽  
Vol 74 (4) ◽  
pp. 763-766 ◽  
Author(s):  
A. A. Schneiter ◽  
B. L. Johnson

Injury to sunflower (Helianthus annuus L.) caused by hail and pests can result in financial losses to producers. This field study was conducted to determine the response of sunflower to different types and levels of physical injury. The information will assist producers in deciding whether to replant or maintain a crop. Studies were conducted at four North Dakota locations. Treatments consisted of stand reduction, terminal bud removal, and top one third of the plant cut off, imposed on either 25 or 50% of an established plant population at five stages of plant development. Plant response to injury varied among treatment stages. When plants were injured at vegetative stages V4 and V8, 98.2 and 93.2% of the treated plants produced multiple branches and heads. When plants were injured during the transition from vegetative to reproductive development (V12, R1), a greater proportion (26.1 and 20.6%, respectively) of the plants died. The majority of the plants injured at stages R1 and R3 neither died nor branched (55.5 and 96.1%, respectively); they continued to live but did not produce seed. Living injured plants reduced total crop yield more than the injured plants that died, since living injured plants continued to compete with uninjured plants for space, light, nutrients and moisture but did not contribute toward yield. Key words: Sunflower, Helianthus annuus L., hail, crop injury


BioScience ◽  
1989 ◽  
Vol 39 (3) ◽  
pp. 189-190
Author(s):  
Richard E. Ford
Keyword(s):  

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