Immunoprecipitation Isolates Multiple TSH Receptor Forms from Human Thyroid Tissue

Thyroid ◽  
1995 ◽  
Vol 5 (2) ◽  
pp. 101-105 ◽  
Author(s):  
R.F. GROSSMAN ◽  
T. BAN ◽  
Q.Y. DUH ◽  
S. TEZELMAN ◽  
G. JOSSART ◽  
...  
Keyword(s):  
Thyroid Tissue ◽  
Tsh Receptor ◽  
Human Thyroid ◽  
Human Thyroid Tissue

STUDIES OF THE TSH RADIORECEPTOR ASSAY

1979 ◽  
Vol 90 (2) ◽  
pp. 217-226 ◽  
Author(s):  
Gildon N. Beall ◽  
Inder J. Chopra ◽  
David H. Solomon ◽  
W. James Irvine ◽  
Sally R. Kruger
Keyword(s):  
Protease Inhibitor ◽  
Specific Binding ◽  
Thyroid Tissue ◽  
Radioreceptor Assay ◽  
Tsh Receptor ◽  
Human Thyroid ◽  
Post Mortem ◽  
Human Thyroid Tissue ◽  
Several Variables ◽  
Bovine Tsh

ABSTRACT We have examined several variables in the reagents and procedures used in the TSH radioreceptor assay, the binding of iodinated TSH to its thyroidal receptor. We found that iodinated bovine TSH (S.A. 30 U/mg) was more effectively bound to receptor than iodinated human TSH (S.A. 7.3 U/mg). Iodination of TSH with the Bolton-Hunter acylation method apparently prevented binding to TSH receptor. Surgically removed human thyroid tissue specifically bound 10.3 ±1.0 (mean ± sem) of added [125I]TSH, but post-mortem human thyroid bound only 3.9 ± 0.4% of [125I]TSH (P< 0.001). Maximal binding of [125I]TSH was found at pH 5.8. Many tissue preparations contained activity, possibly due to proteases, which inactivated TSH, and inclusion of a protease inhibitor, aprotinin, significantly increased specific binding.

Effect of thyroxine and iodide treatment on xenotransplanted human thyroid tissue endogenously stimulated with TSH

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S48-S49
Author(s):  
P.-M. SCHUMM-DRAEGER ◽  
H.J.C. WENISCH ◽  
F.D. MAUL ◽  
C. TIMM
Keyword(s):  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue

Metabolism of 1-131 by Human Thyroid Tissue in Organ Culture.

1966 ◽  
Vol 122 (1) ◽  
pp. 121-126
Author(s):  
W. Hung ◽  
T. Winship ◽  
K. Bowen ◽  
J. C. Houck
Keyword(s):  
Organ Culture ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue

scholarly journals Proteolytic Activity in Extracts from Human Thyroid Tissue.

1964 ◽  
Vol 18 ◽  
pp. 1792-1794
Author(s):  
Gunnar Lundblad ◽  
Janis Immers ◽  
Tore Ledaal ◽  
Hans M. Seip
Keyword(s):  
Proteolytic Activity ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue

Distribution and characterization of steroid hormone receptors in human thyroid tissue

1986 ◽  
Vol 10 (5) ◽  
pp. 737-743 ◽  
Author(s):  
Prabir K. Chaudhuri ◽  
Nili Patel ◽  
Leslye Sandberg ◽  
Richard A. Prinz
Keyword(s):  
Hormone Receptors ◽  
Steroid Hormone ◽  
Thyroid Tissue ◽  
Steroid Hormone Receptors ◽  
Human Thyroid ◽  
Human Thyroid Tissue
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