Impaired Generation of Anti-AKR/Gross Murine Leukemia Virus Cytotoxic T Lymphocytes in Mice Experimentally Infected with MuLV

1996 ◽  
Vol 9 (2) ◽  
pp. 107-119 ◽  
Author(s):  
MICHAEL A. COPPOLA ◽  
WILLIAM R. GREEN ◽  
ROBERT F. RICH
Keyword(s):  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Murine Leukemia

scholarly journals Specificity studies on cytolytic T lymphocytes directed against murine leukemia virus-induced tumors. Analysis of monoclonal cytolytic T lymphocytes.

1982 ◽  
Vol 155 (4) ◽  
pp. 1050-1062 ◽  
Author(s):  
F Plata
Keyword(s):  
T Lymphocytes ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Target Cells ◽  
Cytolytic T Lymphocytes ◽  
Tumor Target ◽  
Induced Tumors ◽  
Definition Of ◽  
Leukemia Viruses ◽  
Murine Leukemia

The specificities of cloned cytolytic T lymphocytes (CTL) were studied for the analysis of CTL populations generated against murine leukemia viruses (MuLV) in H-2 congenic BALB/c (H-2d) and BALB.B (H-2b) mice. In particular, CTL generated in response to tumors induced by Gross MuLV and Friend MuLV were studied; these tumors expressed virus-induced antigens that do not cross-react and that can be distinguished from each other. The systematic study of 92 CTL clones clearly indicated that MuLV-immune CTL were highly heterogeneous with respect to both the intensities of target cell lysis that they mediated and to their specificity of recognition of MuLV-induced tumor target cells. Various categories of CTL clones were identified, ranging from CTL clones tht were tightly H-2 restricted and specific for the immunizing tumor to CTL clones that displayed no discernible patterns of specificity and that attacked a large number of different target cells. In addition, the surface markers of these cloned CTL were defined, and the best conditions for their prolonged maintenance in culture were determined. The present data indicate that future efforts in the definition of target antigens recognized by tumor-specific CTL should be performed with monoclonal lymphocytes.

scholarly journals Thymic targets for Abelson murine leukemia virus are early gamma/delta T lymphocytes.

1991 ◽  
Vol 88 (9) ◽  
pp. 3700-3704 ◽  
Author(s):  
G. D. Holland ◽  
K. Ito ◽  
D. A. Kaehler ◽  
S. Tonegawa ◽  
R. Risser
Keyword(s):  
T Lymphocytes ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Gamma Delta ◽  
Abelson Murine Leukemia Virus ◽  
Murine Leukemia

scholarly journals Mink Cell Focus-Forming Murine Leukemia Virus Infection Induces Apoptosis of Thymic Lymphocytes

2000 ◽  
Vol 74 (17) ◽  
pp. 8119-8126 ◽  
Author(s):  
Fayth K. Yoshimura ◽  
Tao Wang ◽  
Fei Yu ◽  
Hyeong-Reh C. Kim ◽  
Jerrold R. Turner
Keyword(s):  
T Lymphocytes ◽  
Virus Infection ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Mink Cell ◽  
Thymus Cells ◽  
Thymus Size ◽  
Murine Leukemia

ABSTRACT In a previous study we identified the subpopulations of thymus cells that were infected by the lymphomagenic MCF13 murine leukemia virus (MLV) (F. K. Yoshimura, T. Wang, and M. Cankovic, J. Virol. 73:4890–4898, 1999) and observed an effect on thymus size by virus infection. In this report we describe our results which demonstrate that MCF13 MLV infection of thymuses reduced the number of T lymphocytes in this organ. Histological examination showed diffuse lymphocyte depletion, which was most striking in the CD4+CD8+ lymphocyte-enriched cortical zone. Consistent with this, flow cytometric analysis showed that the lymphocytes which were depleted were predominantly the immature CD3−CD4+ CD8+ and CD3+ CD4+CD8+ cells. A comparison of the percentages of live, apoptotic, and dead cells of the gp70+ and gp70− thymic lymphocytes suggested that this effect on thymus cellularity is a result of virus infection. Studies of the survival of thymic T lymphocytes in culture showed that cells from MCF13 MLV-inoculated mice underwent greater apoptosis and death than cells from control animals. Assays for apoptosis included 7-amino-actinomycin D staining, DNA fragmentation, and cleavage of caspase-3 and poly(ADP-ribose) polymerase proenzymes. Our results suggest that apoptosis of thymic lymphocytes by virus infection is an important step in the early stages of MCF13 MLV tumorigenesis.

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