Rat Cytomegalovirus Induces a Temporal Downregulation of Major Histocompatibility Complex Class I Cell Surface Expression

2005 ◽  
Vol 18 (4) ◽  
pp. 607-615 ◽  
Author(s):  
Gerco C. Hassink ◽  
Joanne G. Duijvestijn-Van Dam ◽  
Danijela Koppers-Lalic ◽  
Jacqueline Van Gaans-Van Den Brink ◽  
Daphne Van Leeuwen ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Surface ◽  
Major Histocompatibility Complex Class ◽  
Surface Expression ◽  
Class I ◽  
Cell Surface Expression ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Transcription of Preintegrated HIV-1 cDNA Modulates Cell Surface Expression of Major Histocompatibility Complex Class I via Nef

2011 ◽  
Vol 85 (6) ◽  
pp. 2828-2836 ◽  
Author(s):  
R. D. Sloan ◽  
B. D. Kuhl ◽  
D. A. Donahue ◽  
A. Roland ◽  
T. Bar-Magen ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Surface ◽  
Major Histocompatibility Complex Class ◽  
Surface Expression ◽  
Class I ◽  
Cell Surface Expression ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Hiv 1

Statins Affect Cell-Surface Expression of Major Histocompatibility Complex Class II Molecules by Disrupting Cholesterol-Containing Microdomains

2005 ◽  
Vol 66 (6) ◽  
pp. 653-665 ◽  
Author(s):  
Hedwich F. Kuipers ◽  
Paula J. Biesta ◽  
Tom A. Groothuis ◽  
Jacques J. Neefjes ◽  
A. Mieke Mommaas ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Surface ◽  
Major Histocompatibility Complex Class ◽  
Surface Expression ◽  
Class Ii ◽  
Cell Surface Expression ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Availability of endogenous peptides limits expression of an M3a-Ld major histocompatibility complex class I chimera.

1994 ◽  
Vol 179 (1) ◽  
pp. 155-165 ◽  
Author(s):  
J M Vyas ◽  
R R Rich ◽  
D D Howell ◽  
S M Shawar ◽  
J R Rodgers
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Surface ◽  
Major Histocompatibility Complex Class ◽  
Surface Expression ◽  
Class I ◽  
Dependent Manner ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Endogenous Peptides ◽  
Histocompatibility Complex

Taking advantage of our understanding of the peptide specificity of the major histocompatibility complex class I-b molecule M3a, we sought to determine why these molecules are poorly represented on the cell surface. To this end we constructed a chimeric molecule with the alpha 1 and alpha 2 domains of M3a and alpha 3 of Ld thereby allowing use of available monoclonal antibodies to quantify surface expression. Transfected, but not control, B10.CAS2 (H-2M3b) cells were lysed readily by M3a-restricted monoclonal cytotoxic T lymphocytes. Thus, the chimera bound, trafficked, and presented endogenous mitochondrial peptides. However, despite high levels of M3a-Ld mRNA, transfectants were negative by surface staining. This finding was consistent with inefficient trafficking to the cell surface. Incubation at 26 degrees C, thought to permit trafficking of unoccupied heavy (H) chains, resulted in detectable cell surface expression of chimeric molecules. Incubation with exogenous peptide at 26 degrees C (but not at 37 degrees C) greatly enhanced expression of M3a-Ld molecules in a dose-dependent manner, suggesting stabilization of unoccupied molecules. Stable association of beta 2-microglobulin with the chimeric H chain was observed in labeled cell lysates only in the presence of exogenous specific peptide, indicating that peptide is required for the formation of a ternary complex. These results indicate that surface expression of M3a-Ld is limited largely by the steady-state availability of endogenous peptides. Since most known M3a-binding peptides are N-formylated, native M3a may normally be expressed at high levels only during infection by intracellular bacteria.

scholarly journals Interaction between the CD8 Coreceptor and Major Histocompatibility Complex Class I Stabilizes T Cell Receptor-Antigen Complexes at the Cell Surface

2005 ◽  
Vol 280 (30) ◽  
pp. 27491-27501 ◽  
Author(s):  
Linda Wooldridge ◽  
Hugo A. van den Berg ◽  
Meir Glick ◽  
Emma Gostick ◽  
Bruno Laugel ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Cell Surface ◽  
Major Histocompatibility Complex Class ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Class I ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex
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