As we are moving forward into the modern era of science, several new technologies have revolutionized various branches of science. Techniques of biodiversity conservation, fish biology etc. has also adapted to modern techniques. For a long time, most of the researches in taxonomy, including fisheries science were based on morphology and traditional methods. After the decade of 90’s, slowly severalmolecular markers like RFLP, RAPD, SNP’s etc. made inroad into taxonomy and fisheries. Molecular markers have several applications in the field of livestock improvement and understanding population dynamics to name a few. Since the 2004, a specific molecular marker, generally known as DNA Barcoding for species identification, came up. This molecular marker is a part of mitochondrial genome that encodes for Cytochrome C Oxidase Unit I (also called as COX or COI). It is advantageous because it has been tested across several animal species and it can differentiate species very well. This marker has also been used as a forensic tool to identify the species. In the current paper, we have used this molecular marker to decode evolution of native fishes of Garhwal Himalayan region. Over 350 barcodes were developed and these barcodes were used to for phylogenetic analysis.
Genetic and Chromosomal Differentiation ofRhamdia quelen(Siluriformes, Heptapteridae) Revealed by Repetitive Molecular Markers and DNA Barcoding
