scholarly journals Expression and characterization of Kunitz domain 3 and C-terminal of human tissue factor pathway inhibitor-2

2009 ◽  
Vol 41 (11) ◽  
pp. 948-954 ◽  
Author(s):  
L. Zhu ◽  
J. Wang ◽  
J. Mu ◽  
H. Wang ◽  
C. Zhang ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Human Tissue ◽  
Tissue Factor Pathway Inhibitor ◽  
Kunitz Domain ◽  
Tissue Factor Pathway ◽  
Domain 3

Structural Mechanism for Heparin-Binding of the Third Kunitz Domain of Human Tissue Factor Pathway Inhibitor†,‡

Biochemistry ◽  
2002 ◽  
Vol 41 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Shouhei Mine ◽  
Toshio Yamazaki ◽  
Toshiyuki Miyata ◽  
Saburo Hara ◽  
Hisao Kato
Keyword(s):  
Tissue Factor ◽  
Human Tissue ◽  
Tissue Factor Pathway Inhibitor ◽  
Heparin Binding ◽  
Structural Mechanism ◽  
The Third ◽  
Kunitz Domain ◽  
Tissue Factor Pathway

The second Kunitz domain of human tissue factor pathway inhibitor: cloning, structure determination and interaction with factor Xa 1 1Edited by T. Richmond

1997 ◽  
Vol 269 (3) ◽  
pp. 395-407 ◽  
Author(s):  
Maurits J.M Burgering ◽  
Leon P.M Orbons ◽  
Antoon van der Doelen ◽  
John Mulders ◽  
Henri J.M Theunissen ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Structure Determination ◽  
Human Tissue ◽  
Tissue Factor Pathway Inhibitor ◽  
Factor Xa ◽  
Kunitz Domain ◽  
Tissue Factor Pathway

scholarly journals Characterization of human tissue factor pathway inhibitor variants expressed in Saccharomyces cerevisiae

1993 ◽  
Vol 268 (18) ◽  
pp. 13344-13351
Author(s):  
J.G. Petersen ◽  
G. Meyn ◽  
J.S. Rasmussen ◽  
J. Petersen ◽  
S.E. Bjørn ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Tissue Factor ◽  
Human Tissue ◽  
Tissue Factor Pathway Inhibitor ◽  
Tissue Factor Pathway

scholarly journals Engineering Kunitz Domain 1 (KD1) of Human Tissue Factor Pathway Inhibitor-2 to Selectively Inhibit Fibrinolysis

2010 ◽  
Vol 286 (6) ◽  
pp. 4329-4340 ◽  
Author(s):  
Madhu S. Bajaj ◽  
Godwin I. Ogueli ◽  
Yogesh Kumar ◽  
Kanagasabai Vadivel ◽  
Gregory Lawson ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Human Tissue ◽  
Tissue Factor Pathway Inhibitor ◽  
Kunitz Domain ◽  
Tissue Factor Pathway

Factor Xa Enhances the Binding of Tissue Factor Pathway Inhibitor to Acidic Phospholipids

1996 ◽  
Vol 75 (05) ◽  
pp. 796-800 ◽  
Author(s):  
Sanne Valentin ◽  
Inger Schousboe
Keyword(s):  
Tissue Factor ◽  
Tissue Factor Pathway Inhibitor ◽  
Factor Xa ◽  
Microtitre Plate ◽  
C Terminus ◽  
Microtitre Plate Assay ◽  
Kunitz Domain ◽  
Tissue Factor Pathway ◽  
Acidic Phospholipids

SummaryIn the present study, the interaction between tissue factor pathway inhibitor (TFPI) and phospholipids has been characterized using a microtitre plate assay. TFPI was shown to bind calcium-independently to an acidic phospholipid surface composed of phosphatidylserine, but not a surface composed of the neutral phosphatidylcholine. The interaction was demonstrated to be dependent on the presence of the TFPI C-terminus. The presence of heparin (1 U/ml, unfractionated) was able to significantly reduce the binding of TFPI to phospholipid. The interaction of TFPI with phosphatidylserine was significantly decreased in the presence of calcium, but this was counteracted, and even enhanced, following complex formation of TFPI with factor Xa prior to incubation with the phospholipid surface. Moreover, a TFPI variant, not containing the third Kunitz domain and the C-terminus, was unable to bind to phospholipid. However, following the formation of a TFPI/factor Xa-complex this TFPI variant was capable of interacting with the phospholipid surface. This indicates that the role of factor Xa as a TFPI cofactor, at least in part, is to mediate the binding of TFPI to the phospholipid surface.

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