Variability of the 5S and 45S rDNA Sites in Passiflora L. Species with Distinct Base Chromosome Numbers

The rDNA sites are useful chromosome landmarks and can provide valuable information for species identification and species relationships. In this study, we investigated the distribution of 5S and 45S rDNA sites in 29 species of Begonia sect. Coelocentrum Irmsch. using a two-colour fluorescence in situ hybridization (FISH) technique. This is the first report of chromosomal rDNA mapping in Begonia species. The analyzed species showed considerable diversity in rDNA distribution patterns. The 45S rDNA signals are always located in terminal regions on 1−4 chromosomes, while 5S rDNA signals are mainly located at proximal regions on 2−8 chromosomes, varying from specific major signals to highly dispersed minor signals. Based on rDNA FISH patterns, most of the investigated species could be distinguished from each other and species relationships were identified. In addition, the results provided clear proof that B. huangii is of hybrid origin and the triploid B. longgangensis was allotriploid rather than autotriploid as suggested before. The data will provide a useful reference for evaluation, conservation and utilization of the natural resources of the mega-diverse genus Begonia.

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Identification of 5S and 45S rDNA sites in Chrysanthemum species by using oligonucleotide fluorescence in situ hybridization (Oligo-FISH)

Molecular Biology Reports ◽

10.1007/s11033-020-06102-1 ◽

2021 ◽

Vol 48 (1) ◽

pp. 21-31

Author(s):

Jun He ◽

Sisi Lin ◽

Zhongyu Yu ◽

Aiping Song ◽

Zhiyong Guan ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

45S Rdna ◽

Rdna Sites ◽

5S And 45S Rdna

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Linked 5S and 45S rDNA Sites Are Highly Conserved through the Subfamily Aurantioideae (Rutaceae)

Cytogenetic and Genome Research ◽

10.1159/000350695 ◽

2013 ◽

Vol 140 (1) ◽

pp. 62-69 ◽

Cited By ~ 14

Author(s):

A.E. Barros e Silva ◽

W. dos Santos Soares Filho ◽

M. Guerra

Keyword(s):

45S Rdna ◽

Rdna Sites ◽

5S And 45S Rdna

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Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.128.5.0736 ◽

2003 ◽

Vol 128 (5) ◽

pp. 736-740 ◽

Cited By ~ 12

Author(s):

Young A Choi ◽

Ryutaro Tao ◽

Keizo Yonemori ◽

Akira Sugiura

Keyword(s):

Fluorescent In Situ Hybridization ◽

Diploid Species ◽

5S Rdna ◽

Diospyros Kaki ◽

45S Rdna ◽

Multicolor Fish ◽

Rdna Probe ◽

Rdna Sites ◽

5S And 45S Rdna

5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on different chromosomes of Diospyros species tested, suggesting that 5S and 45S rDNA sites can be used as chromosome markers in Diospyros. The number of 5S rDNA sites varied with the Diospyros species. More 5S rDNA sites were observed in four diploid species native to Southern Africa than in three Asian diploid species. The former had four or six 5S rDNA sites while the latter had two. Three Asian polyploidy species had four to eight 5S rDNA sites. Among the Asian species, the number of 5S rDNA sites seemed to increase according to ploidy level of species. These features of 5S rDNA sites were very similar to those of 45S rDNA sites in Diospyros. Phylogenetic relationship between D. kaki and wild species tested are discussed based on the number and chromosomal distribution of 5S and 45S rDNA.

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