Improved Method for Measurement of delta-Aminolevulinic Acid Dehydratase Activity of Human Erythrocytes

1971 ◽  
Vol 17 (10) ◽  
pp. 1038-1041 ◽  
Author(s):  
Helen B Burch ◽  
Abraham L Siegel
Keyword(s):  
Aminolevulinic Acid ◽  
Current Method ◽  
Mercury Salt ◽  
Improved Method ◽  
Substrate Solution ◽  
Color Development ◽  
Enzyme Substrate ◽  
Acid Dehydratase ◽  
Dehydratase Activity ◽  
A Current

Abstract We have evaluated and modified a current method for estimating δ-aminolevulinic acid dehydratase (5-aminolaevulinate hydro-lyase, EC 4.2.1.24) activity in blood, so as to adequately control the pH of the enzyme-substrate solution at the optimum throughout the incubation period. This has improved the sensitivity and reliability of the assay. Also, N-ethylmaleimide has been substituted for a potentially hazardous mercury salt, used to remove sulfhydryl groups before color development with a modified Ehrlich’s reagent.

scholarly journals Erythrocyte D -Aminolevulinic Acid Dehydratase (Ala-D) Activity and Blood Lead Level (Pb-B) of Tortoise (Testudo Hemanni, Gmel.) of Vicinity of Lead and Zinc Smelter “Trepca” in Kosova

1998 ◽  
Vol 1 (1) ◽  
pp. 24-25
Author(s):  
I. Elezaj ◽  
D.A. Rozhaja
Keyword(s):  
Aminolevulinic Acid ◽  
Lead Level ◽  
Blood Lead ◽  
Significant Negative Correlation ◽  
Polluted Environment ◽  
Zinc Smelter ◽  
Acid Dehydratase ◽  
Lead And Zinc ◽  
Testudo Hermanni ◽  
Dehydratase Activity

In comparison with control animals, a significant negative correlation (r = 0.77 -), between lead level and the activity of daminolevulinic acid dehydratase activity in the blood of land turtle (Testudo hermanni, Gmel.) caught in five different localities from heavy polluted environment of lead and zinc smelter “Trepca” was found. The concentration ratio of lead level in blood between control animals and those from the vicinity of smelter was 10:160 % and ratio of ALA-D activity was 10:18,3 U/LE.

Effects of Cadmium, Lead, Mercury and Zinc on °-Aminolevulinic Acid Dehydratase Activity from Radish Leaves

2002 ◽  
Vol 45 (1) ◽  
pp. 85-89 ◽  
Author(s):  
V.M. Morsch ◽  
M.R.C. Schetinger ◽  
A.F. Martins ◽  
J.B.T. Rocha
Keyword(s):  
Aminolevulinic Acid ◽  
Acid Dehydratase ◽  
Cadmium Lead ◽  
Dehydratase Activity
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