Coulometric Determination of Activity of Acid or Alkaline Phosphatases in Serum

1972 ◽  
Vol 18 (6) ◽  
pp. 503-508 ◽  
Author(s):  
Marvin A Brooks ◽  
William C Purdy
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Detection System ◽  
Constant Current ◽  
Coulometric Determination ◽  
Citrate Buffer ◽  
Serum Alkaline Phosphatase Activity ◽  
Clin Pathol

Abstract A constant-current coulometric technique is described for measuring acid or alkaline phosphatase activity in serum. Electrogenerated bromine is used as the titrant of phenol enzymatically released from phenyl phosphate. A biamperometric end-point detection system is used, with an applied potential of 135 mV. Titrations are performed at pH 1 with use of a generating electrolyte of 0.5 molar KBr in 0.05 molar H2SO4. A procedure is described for determining serum alkaline phosphatase activity by use of 2-amino-2-methyl-1-propanol (0.25 mol/liter, pH 10.25) with Mg2+ activator (1 mmol/liter), and for determining serum acid phosphatase activity in citrate buffer (0.1 mol/liter, pH 4.9). Results compare favorably with those of the method of Kind and King [J. Clin. Pathol. 7, 322 (1954)].

Alkaline phosphatase activity in the plasma of children and adolescents.

1977 ◽  
Vol 23 (3) ◽  
pp. 469-472 ◽  
Author(s):  
G A Fleisher ◽  
E S Eickelberg ◽  
L R Elveback
Keyword(s):  
Alkaline Phosphatase ◽  
Public Schools ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Intraclass Correlation ◽  
Serum Alkaline Phosphatase Activity ◽  
Upper Limits ◽  
Prepubertal Girls

Abstract We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in boys. Adult values were not reached until six to eight years later. In 180 pairs of siblings, a significant intraclass correlation was noted. A possible role of alkaline phosphatase in the regulation of protein synthesis is suggested.

Effects of various fibrates on serum alkaline phosphatase activity

2002 ◽  
Vol 165 (1) ◽  
pp. 187-188 ◽  
Author(s):  
Emanuel Ganotakis ◽  
Vasilios Tsimihodimos ◽  
Eleni Bairaktari ◽  
Evagelos Rizos ◽  
Vasilios Athyros ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity

AutoAnalyzer Assay for Serum Alkaline Phosphatase Activity, with Sodium Thymolphthalein Monophosphate as Substrate

1973 ◽  
Vol 19 (1) ◽  
pp. 103-105 ◽  
Author(s):  
Gary J Proksch ◽  
Dean P Bonderman ◽  
John A Griep
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Flow Diagram ◽  
Good Precision ◽  
Serum Alkaline Phosphatase Activity ◽  
Manual Method ◽  
Automated Method ◽  
Simple Flow

Abstract An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation

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