Assessment of fetal lung maturity by colorimetric phospholipid determination without digestion.

1979 ◽  
Vol 25 (5) ◽  
pp. 793-796 ◽  
Author(s):  
H G Worth ◽  
D J Wright
Keyword(s):  
Statistical Analysis ◽  
Amniotic Fluid ◽  
Total Phospholipid ◽  
Fetal Lung ◽  
Routine Laboratory ◽  
Fetal Lung Maturity ◽  
Laboratory Results ◽  
Original Procedure ◽  
Lung Maturity

Abstract A previously described method for the determination of phospholipid concentration in amniotic fluid without digestion has been modified to make it more suitable for use in a routine laboratory. Results compare well with those by the original procedure. Results from nearly 70 amniotic fluids, collected at delivery, were compared with lecithin/sphingomyelin (L/S) ratios determined on the same fluids. Statistical analysis of these data showed that for the prediction of lung immaturity, determination of total phospholipid concentration was at least as good as the L/S ratio.

Is the L/S Ratio Really a Lecithin/Sphingomyelin Ratio?

1979 ◽  
Vol 16 (1-6) ◽  
pp. 184-190 ◽  
Author(s):  
Coral G. Duck-Chong ◽  
Louise M. Brown
Keyword(s):  
Amniotic Fluid ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Original Procedure ◽  
Cold Acetone ◽  
Lung Maturity

Fetal lung maturity is commonly assessed by determining the ratio of lecithin/sphingomyelin in centrifuged amniotic fluid. In a variety of chromatographic systems currently used for the routine determination of the lecithin/sphingomyelin ratio, including the systems recommended in the original procedure, at least one and frequently two additional phospholipids, normally present in amniotic fluid, tend to chromatograph between or overlapping with lecithin and/or sphingomyelin. These phospholipids have been tentatively identified as phosphatidylserine and phosphatidylinositol. The extra phospholipids contribute significantly to the routine lecithin/sphingomyelin ratio with considerable variation between individual cases. Treatment of dried lipid extracts with cold acetone before chromatography, as suggested in the original lecithin/sphingomyelin ratio procedure, does not remove the interfering phospholipids.

Determination of fetal lung maturity by fluorescence polarization of amniotic fluid

1978 ◽  
Vol 130 (7) ◽  
pp. 782-787 ◽  
Author(s):  
Thomas A. Blumenfeld ◽  
Raymond I. Stark ◽  
L. Stanley James ◽  
John D. George ◽  
Inga Dyrenfurth ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity

scholarly journals DETERMINATION OF FETAL LUNG MATURITY BY FLUORESCENT POLARIZATION (FP) OF AMNIOTIC FLUID

1977 ◽  
Vol 11 (4) ◽  
pp. 413-413 ◽  
Author(s):  
John F Vogt ◽  
Steven A Golde ◽  
Luis A Cabal ◽  
Steven Gabbe ◽  
Joan E Hedgman
Keyword(s):  
Amniotic Fluid ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity
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