Use or abuse of the fluorescence polarization measurement of amniotic fluid for determination of fetal lung maturity.

1983 ◽  
Vol 29 (8) ◽  
pp. 1565-1565 ◽  
Author(s):  
D O Gebhardt
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Fetal Lung ◽  
Polarization Measurement ◽  
Fetal Lung Maturity ◽  
Fluorescence Polarization Measurement ◽  
Lung Maturity

Determination of fetal lung maturity by fluorescence polarization of amniotic fluid

1978 ◽  
Vol 130 (7) ◽  
pp. 782-787 ◽  
Author(s):  
Thomas A. Blumenfeld ◽  
Raymond I. Stark ◽  
L. Stanley James ◽  
John D. George ◽  
Inga Dyrenfurth ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Amniotic fluid fluorescence polarization value at physiological temperature: a marked improvement in assessing fetal lung maturity.

1983 ◽  
Vol 29 (2) ◽  
pp. 264-267 ◽  
Author(s):  
G Barkai ◽  
S Mashiach ◽  
M Modan ◽  
D M Serr ◽  
D Lanir ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Room Temperature ◽  
Fetal Lung ◽  
Hyaline Membrane Disease ◽  
P Value ◽  
Physiological Temperature ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Abstract Determination of fetal lung maturity by measurement of the fluorescence polarization (P) value of the amniotic fluid at room temperature has become the method of choice in an increasing number of perinatal units because of its simplicity and relatively high predictive value. Nevertheless, its power to discriminate between cases with and without hyaline membrane disease (HMD) needs improvement. To this end, we assessed the discriminative power of the P value at the physiological temperature of 37 degrees C (P37) as compared with the power at 25 degrees C (P25). The study group consisted of 288 consecutive cases at risk for preterm delivery. Samples from all 288 cases were measured at 25 degrees C and samples from 112 of these were measured concurrently at 37 degrees C as well. HMD occurred in 27 infants of the total group, nine of whom belonged to the subgroup tested at both temperatures. When sensitivity was fixed at 100% the specificity of P37 was 97% as compared to 79% for P25 (p less than 0.001). The percentage of cases with infants free of HMD who had borderline P values was also significantly smaller: 1% vs 21%, respectively (p less than 0.001). Although data on more HMD cases are needed to establish the precise threshold of lung maturity for P37, we conclude that P37 is a considerably better discriminator for fetal lung maturity determination than P25.

Improved fluorescence polarization assay for use in evaluating fetal lung maturity. III. Retrospective clinical evaluation and comparison with the lecithin/sphingomyelin ratio.

1986 ◽  
Vol 32 (2) ◽  
pp. 260-264 ◽  
Author(s):  
E R Ashwood ◽  
J F Tait ◽  
C A Foerder ◽  
R W Franklin ◽  
T J Benedetti
Keyword(s):  
Respiratory Distress ◽  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Clinical Laboratory ◽  
Clinical Performance ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Fluorescence Polarization Assay ◽  
A Prospective Study ◽  
Lung Maturity

Abstract We clinically evaluated, retrospectively, our improved fluorescence polarization assay for fetal lung maturity. The procedure requires 0.5 mL of amniotic fluid and a standard clinical laboratory fluorescence polarimeter (TDx Analyzer, Abbott Laboratories). We measured the L/S ratios for 93 freshly collected amniotic fluids, uncontaminated with blood or meconium, collected within three days of delivery. The fluids were stored frozen for eight to 32 months, then thawed and assayed for net fluorescence polarization. Fourteen of the infants developed respiratory distress syndrome; five, transient tachypnea of the newborn; and 74, no respiratory distress. The polarization assay and lecithin/sphingomyelin ratio had equivalent receiver operating characteristic curves, indicating no difference in their clinical performance. Although a prospective study with fresh amniotic fluid specimens will be necessary to establish a definitive reference range, the present study shows that this assay can be used to rapidly predict fetal lung maturity.

Improved fluorescence polarization assay for use in evaluating fetal lung maturity. II. Analytical evaluation and comparison with the lecithin/sphingomyelin ratio.

1986 ◽  
Vol 32 (2) ◽  
pp. 255-259 ◽  
Author(s):  
C A Foerder ◽  
J F Tait ◽  
R W Franklin ◽  
E R Ashwood
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Intensity ◽  
Fluorescence Polarization ◽  
Clinical Laboratory ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Significant Difference ◽  
Endogenous Fluorescence ◽  
Fluorescence Polarization Assay ◽  
Lung Maturity

Abstract We assessed the analytical performance of an improved fluorescence polarization assay for use in evaluating fetal lung maturity and compared results with the lecithin/sphingomyelin ratio. During a three-month period 150 patients' samples were assayed by clinical laboratory personnel with both techniques. Values for the lecithin/sphingomyelin ratio correlate closely with net fluorescence polarization values (r = -0.85), less closely with net fluorescence intensity (r = 0.65). Background fluorescence intensity and polarization varied widely, indicating a need to correct measurements for endogenous fluorescence. Net fluorescence polarization values have a CV of 0.32% within-run, 1.07% between-day. A comparison of two amniotic fluid centrifugation procedures showed no significant difference in such values. For both methods, however, such values are slightly but significantly higher than those obtained for amniotic fluids without prior centrifugation. Short-term storage (less than 30 days) of uncentrifuged amniotic fluid samples at -20 degrees C does not significantly affect results.

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