Use of N-methyl-D-glucamine as buffer in the determination of serum alkaline phosphatase activity.

1981 ◽  
Vol 27 (10) ◽  
pp. 1729-1732 ◽  
Author(s):  
V Chromý ◽  
L Zahradnícek ◽  
J Voznícek
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Reaction Conditions ◽  
Serum Alkaline Phosphatase Activity ◽  
Nitrophenyl Phosphate ◽  
Optimum Reaction ◽  
Assay Conditions

Abstract We describe a method for determining serum alkaline phosphatase activity with use of N-methyl-D-glucamine buffer, Na+ is a definite activator, whereas NH4+ and Li+ inhibit enzyme activity. Optimum reaction conditions are: methylglucamine buffer, 0.35 mol/L, pH 10.2 +/- 0.1 (30 degrees C); NaCl, 70 mmol/L; MgCl2, 0.5 mmol/L; disodium 4-nitrophenyl phosphate, 15 mmol/L; reaction temperature, 30 degrees C; reaction time, 2 min. The assay conditions are optimum for all human serum isoenzymes.

Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer.

1976 ◽  
Vol 22 (10) ◽  
pp. 1737-1740 ◽  
Author(s):  
R C Elser
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Blood Donors ◽  
Serum Alkaline Phosphatase ◽  
Thymol Blue ◽  
Serum Alkaline Phosphatase Activity ◽  
Nitrophenyl Phosphate ◽  
Stable Substrate ◽  
High Concentrations

Abstract Thymol blue monophosphate is evaluated as a substrate for use in kinetic bichromatic analysis of serum alkaline phosphatase activity. The effect of abnormally high concentrations of hemoglobin, bilirubin, or chylomicrons is negligible. The expected range for ambulatory adults established by assaying sera from 206 blood donors, was 12-44 U/lliter at 37 degrees C. Comparison of the method with a commonly used p-nitrophenyl phosphate method yielded a correlation coefficient, r, of 0.9949. Advantages of the method are the reduced cost per test and the exceptionally stable substrate.

Continuous Spectrophotometric Measurement of Serum Alkaline Phosphatase Activity

1967 ◽  
Vol 13 (7) ◽  
pp. 608-610 ◽  
Author(s):  
George N Bowers ◽  
Mari-Lou Kelley ◽  
Robert B McComb
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Molar Absorptivity ◽  
Reaction Conditions ◽  
Spectrophotometric Measurement ◽  
Serum Alkaline Phosphatase Activity ◽  
Alkaline Phosphatase Assay ◽  
Phosphatase Assay

Abstract Findings with 9 self-indicating phenolic substrates for alkaline phosphatase assay by continuous spectrophotometric technic demonstrate that not only specific reaction conditions, e.g., buffer type, molarity, and pH, contribute significantly to the final observed "Sensitivity" attributed to any 1 substrate but also the following variables must be considered: (1) the enzyme's tissue and species source; (2) the molar absorptivity difference between the substrate and its dephosphorylated product; and (3) the molecular activity.

Fluoride therapy for osteoporosis: Characterization of the skeletal response by serial measurements of serum alkaline phosphatase activity

Metabolism ◽  
1987 ◽  
Vol 36 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Sally M.G. Farley ◽  
Jon E. Wergedal ◽  
Lynna C. Smith ◽  
Mark W. Lundy ◽  
John R. Farley ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity ◽  
Serial Measurements

Alkaline phosphatase activity in the plasma of children and adolescents.

1977 ◽  
Vol 23 (3) ◽  
pp. 469-472 ◽  
Author(s):  
G A Fleisher ◽  
E S Eickelberg ◽  
L R Elveback
Keyword(s):  
Alkaline Phosphatase ◽  
Public Schools ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Intraclass Correlation ◽  
Serum Alkaline Phosphatase Activity ◽  
Upper Limits ◽  
Prepubertal Girls

Abstract We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in boys. Adult values were not reached until six to eight years later. In 180 pairs of siblings, a significant intraclass correlation was noted. A possible role of alkaline phosphatase in the regulation of protein synthesis is suggested.

A reference method for measurement of alkaline phosphatase activity in human serum.

1983 ◽  
Vol 29 (5) ◽  
pp. 751-761 ◽  
Author(s):  
N W Tietz ◽  
C A Burtis ◽  
P Duncan ◽  
K Ervin ◽  
C J Petitclerc ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Human Serum ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Zinc Sulfate ◽  
Metal Ion ◽  
Reference Method ◽  
Reaction Conditions ◽  
Factors Affecting ◽  
Nitrophenyl Phosphate

Abstract We present an official AACC reference method for the measurement of alkaline phosphatase, the culmination of optimization experiments conducted by a group of independent laboratories. The details of this method and evaluation of factors affecting the measurement are described. A metal ion buffer has been incorporated that maintains optimal and constant concentrations of zinc(II) and magnesium(II) ions. Final reaction conditions are: pH (30 degrees C), 10.40 +/- 0.05; 2-amino-2-methyl-1-propanol buffer, 0.35 mol/L; 4-nitrophenyl phosphate, 16.0 mmol/L; magnesium acetate, 2.0 mmol/L; zinc sulfate, 1.0 mmol/L; and N-(2-hydroxyethyl)ethylenediaminetriacetic acid, 2.0 mmol/L.

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