Interference of fetal hemoglobin and labile glycosylated hemoglobin with measurements of glycosylated hemoglobin.

1983 ◽  
Vol 29 (3) ◽  
pp. 543-545 ◽  
Author(s):  
R W Yatscoff ◽  
G J Tevaarwerk ◽  
C L Clarson ◽  
L M Warnock
Keyword(s):  
Glycosylated Hemoglobin ◽  
Accurate Determination ◽  
Fetal Hemoglobin ◽  
Hemoglobin Concentration ◽  
Exchange Chromatography ◽  
Affinity Column ◽  
Affinity Column Chromatography ◽  
Hemoglobin A1 ◽  
Total Hemoglobin Concentration

Abstract We examined the effect of fetal hemoglobin and labile glycosylated hemoglobin on a number of diverse methods used to measure glycosylated hemoglobin. Samples were supplemented with various amounts of cord blood to give proportions of fetal hemoglobin ranging from 1 to 20% of total hemoglobin concentration. Procedures in which the separation of hemoglobin A1 from the major hemoglobin A fraction is based on differences in ionic properties (cation-exchange chromatography and electrophoresis) are subject to interference by fetal hemoglobin, whereas procedures that base the quantitation on other properties (colorimetry and affinity column chromatography) are not. The same procedures that are affected by the presence of fetal hemoglobin are also subject to interference by labile glycosylated hemoglobin. We conclude that the affinity chromatographic and colorimetric methods may give a more nearly accurate determination of glycosylated hemoglobin.

Results of routine determination of clinically significant hemoglobin derivatives by multicomponent analysis.

1986 ◽  
Vol 32 (6) ◽  
pp. 972-978 ◽  
Author(s):  
A Zwart ◽  
E J van Kampen ◽  
W G Zijlstra
Keyword(s):  
Pulmonary Disease ◽  
Fetal Hemoglobin ◽  
Hemoglobin Concentration ◽  
Major Surgery ◽  
Chemical Laboratory ◽  
Total Hemoglobin ◽  
Clinically Significant ◽  
Blood Specimens ◽  
Total Hemoglobin Concentration

Abstract Our recently developed multiwavelength method for multi-component analysis of hemoglobin (Hb) derivatives (Clin Chem 1984;30:373-379) was adapted for routine use in the clinical chemical laboratory. The method was applied in 4066 determinations on blood specimens from patients awaiting major surgery (n = 3863) or visiting the outpatient department for pulmonary disease (n = 203). Mean total hemoglobin concentration was 141 (SD 14) g/L. The proportion of HbCO was slightly to moderately increased (1.5-10.0%) in 36.5% of all patients; in a few cases it was as high as 15%. Mean methemoglobin was 0.4% (SD 0.2%) in the surgical patients, but 1.5% (SD 0.8%) in the patients with pulmonary disease. In some patients of the latter group the proportion of methemoglobin amounted to 5%. Sulfhemoglobin was found less than 0.4% in all specimens. Interference by paraproteins and by increased concentrations of bilirubin and lipids in plasma was easily detected by means of the performance checks provided by the spectrophotometer (an HP 8451 UV/Vis). The method is equally suitable for measuring blood samples containing fetal hemoglobin.

New spectrophotometric method for the determination of hemoglobin A1 compared with a microcolumn technique.

1982 ◽  
Vol 28 (1) ◽  
pp. 96-99 ◽  
Author(s):  
O Wålinder ◽  
G Ronquist ◽  
P J Fager
Keyword(s):  
Phytic Acid ◽  
Spectrophotometric Method ◽  
Healthy Subjects ◽  
Large Scale ◽  
Glycosylated Hemoglobin ◽  
Standard Curve ◽  
Sugar Moiety ◽  
Hemoglobin A1 ◽  
The Mean

Abstract We compared a spectrophotometric kit method (Glycospec) for determination of glycosylated hemoglobin (HbA1) with a microcolumn kit method (Bio-Rad). The Glycospec method is based on the change in absorbance when phytic acid binds to hemoglobin A. With glycosylated hemoglobin there is no such change because the binding is blocked by the sugar moiety. Inter-assay CVs were 2-6% for both methods. In healthy subjects the mean (+/- SD) value for HbAl was about 1% higher with the spectrophotometric than the microcolumn method. For samples from 122 diabetics the correlation between values for HbAl obtained with the two methods was acceptable (r = 0.89), although the spectrophotometric technique yielded 2-4% higher values, a difference at least partly due to the absence of 2,3-diphosphoglycerate from the spectrophotometric standards. Adding 1.8 mmol of it per liter to these standards caused displacement of the standard curve; HbAl values then agreed well with those of the microcolumn method. The spectrophotometric procedure is easily automated, and therefore is well suited for large-scale analyses if problems with standards and calibration can be solved.

Molar absorptivities of human hemoglobin in the visible spectral range

1983 ◽  
Vol 54 (5) ◽  
pp. 1287-1291 ◽  
Author(s):  
W. G. Zijlstra ◽  
A. Buursma ◽  
A. Zwart
Keyword(s):  
Human Blood ◽  
Hemoglobin Concentration ◽  
Visible Spectral Range ◽  
Human Hemoglobin ◽  
Spectrophotometric Methods ◽  
Total Hemoglobin ◽  
Wide Range ◽  
Different Types ◽  
Total Hemoglobin Concentration

In a recent paper, Burkhard and Barnikol (J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 52: 124–130, 1982) claimed that the absorption spectra of human (oxy-)hemoglobin are dependent on the total hemoglobin concentration (CHb) and it is suggested that this might also be the case with cyanmethemoglobin (HiCN). Such relationships would invalidate the widely used spectrophotometric methods for the determination of total hemoglobin and the fractions of various hemoglobin derivatives in human blood. Although Burkhard and Barnikol's findings are rather improbable considering earlier data, we measured the millimolar absorptivities of oxyhemoglobin (epsilon HbO2) and cyanmethemoglobin (epsilon HiCN) at various wavelengths over a wide range of concentrations (CHb approximately equal to 0.004–10 mmol x 1(-1)), using two different types of spectrophotometers. epsilon HbO2 and epsilon HiCN proved to be independent of CHb. Moreover the values obtained confirmed those in the earlier literature, whereas those of Burkhard and Barnikol are some 30% higher. Consequently there is no reason to doubt the validity of the generally accepted millimolar absorptivities of human hemoglobin.

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