COMPARATIVE TEST OF FLAVONOIDS AND SAPONINS BETWEEN LEAVES AND FLOWERS OF KNOBS (Gomphrena globosa l.) AS ANTI-NAUSEA AND ANTIOXIDANT THERAPY IN CANCER PATIENTS UNDERGOING CHEMOTHERAPY

Cancer is one of the leading causes of death in the world today. In 2018, there were 18.1 million new cases of cancer with a death rate of 9.6 million. Chemotherapy is one way of cancer treating. Effects of chemotherapy include gastrointestinal symptoms such as nausea, vomiting, stomatitis and myelo suppression in the form of anemia, leukopenia, and thrombocytopenia. Symptoms of nausea and vomiting are one of the most serious side effects. This condition can cause patient choose to stop therapy. Discontinuation of therapy has the potential to increase cancer progression. If not treated quickly, it will cause malnutrition. The knob plant (Gomhprena globosa l.) is a nutritious plant that has been used in traditional medicine. Knobs have potential as antioxidants of it flavonoids. The flavonoid and saponin compounds can accelerate the digestive system and act as an anti-nausea, making it suitable as therapy for patients with chemotherapy. This study aims to compare the flavonoid and saponin between the leaves and knob flowers which are best used as anti-nausea products. The research was conducted at the Chemical Laboratory of MAN 1 Gresik. The extraction method used in this is maceration. The steps for testing flavonoids and saponins on knob leaves and flowers can be done by pounding the leaves and knob flowers, labeling 4 test tubes, namely, F flowers for the Flavonoid test, S flowers for the Saponins test, F leaves for the Flavonoid test, S leaves for the Saponins test. Then, add 1 ml of leaf and flower extracts into test tube, add magnesium tape to the test tube labeled F leaves and F flowers, add 1 ml of concentrated HCl into the test tube labeled F, then enter 1 ml of HCl 2N on the test tube labeled S leaves and S flowers. Based on this study, can be concluded that knob leaves have higher flavonoids and saponins. Fact, knob leaves produce more yellow color. In the saponin test, the leaf had more foam than the flower. Therefore, knob leaves can be used as anti-nausea and antioxidants in cancer patients with chemotherapy.

PELATIHAN DAN PENDAMPINGAN DEMO INSTRUMENTASI BAGI MAHASISWA SEBAGAI BAGIAN GOOD LABORATORY PRACTICE

ABSTRAKLaboratorium kimia merupakan salah satu jenis laboratorium yang memiliki resiko sangat berbahaya dalam penyelenggaraan pendidikan, penelitian dan/atau pengabdian kepada masyarakat, sehingga laboratorium harus dikelola dan digunakan secara baik dan bijaksana. Pengoperasian peralatan sebagai salah satu aktivitas Good Laboratory Practice (GLP) merupakan aktivitas secara rutin dilakukan di laboratorium kimia. GLP dapat mewujudkan keselamatan kerja di laboratorium dan meminimalisir kerusakan peralatan akibat kesalahan operasi dan pemeliharaan instrumen. Kegiatan pengabdian ini adalah demo peralatan/instrumentasi Laboratorium Kimia Analitik FMIPA–Universitas Mataram. Metode kegiatan yang dilakukan yaitu pelatihan dan pendampingan yang berupa demo peralatan/instrumentasi meliputi kromatografi gas–spektroskopi massa (GC-MS), kromatografi cair kinerja tinggi (KCKT), spektrometer serapan atom (SSA) dan infra merah (FTIR). Kegiatan ini dilakukan untuk meningkatkan keterampilan dasar mitra dalam pengoperasian instrumentasi tersebut. Mitra kegiatan adalah 20 mahasiswa program studi Kimia semester lanjut (5-8). Keberhasilan kegiatan diukur dengan membandingkan skor tes mitra sebelum dan setelah kegiatan dilakukan. Kegiatan pengabdian berlangsung baik dan menghasilkan peningkatan pemahaman serta keterampilan mitra dalam mengoperasikan instrumen yang ditunjukkan dengan peningkatan skor tes mitra. Kegiatan ini diharapkan dapat mewujudkan GLP sehingga kegiatan penelitian dilakukan dengan aman dan efisien. Kata kunci: pelatihan; instrumen; good laboratory practise; mahasiswa. ABSTRACTChemical laboratory is one type of laboratory that has a very dangerous risk in the implementation of education, research and/or community service, so the laboratory must be managed and used properly and wisely. Equipment operation as one of the Good Laboratory Practice (GLP) activities is a routine activity carried out in chemical laboratory. GLP could realize work safety in laboratory and minimize instrument operation and maintenance mistake This community service activity was a demonstration of instrumentation of the Analytical Chemistry Laboratory, FMIPA–University of Mataram. The method of activity carried out are training and assistance in form of instrument demo including gas chromatography–mass spectroscopy (GC-MS), high performance liquid chromatography (HPLC or HPLC), atomic absorption spectrometer (SSA or AAS) and infrared spectrometer (FTIR). This activity is carried to improve basic skill of partner in operating of instruments. The activity partners are 20 students of the advanced semester (5-8th semester) in Departement of Chemistry. The success of activity was measured by comparing the partner’s test score before and after the activity was carried out.The service activity went well and could increase the partners' understanding and skills in operating the instrument. This activity is expected to realize the GLP so that research activities are carried out safely and efficiently. Keywords: training; instrument; good laboratory practice; student.

Effects of Ginger and Garlic on the Proximate and Mineral Compositions of Differently Processed Nile Tilapia (Oreochromis niloticus)

Freshly caught Nile Tilapia (Oreochromis niloticus) samples were obtained from Sabiyel Lake in Aliero Local Government Area of Kebbi State. Oreochromis niloticus samples were descaled, degutted, cut into chunks and rinsed with clean water at Fisheries Laboratory, Department of Forestry and Fisheries, Kebbi State University of Science and Technology, Aliero. The samples were subjected to different treatments designated as; T1 (oven drying), T2 (Frying), T3 (smoking), T4 (oven-dried + 5% ginger-garlic), T5 (Fried + 5% ginger-garlic), T6 (smoked + 5% ginger-garlic), T7 (oven-dried + 10% ginger-garlic), T8 (Fried + 10% ginger-garlic), T9 (smoked + 10% ginger-garlic), T10 (oven-dried + 15% ginger-garlic), T11 (Fried + 15% ginger-garlic), T12 (smoked + 15% ginger-garlic). The processed samples were taken to Agric. Chemical Laboratory, Faculty of Agriculture, Usmanu Danfodio University Sokoto, Nigeria, for the determination of proximate and mineral compositions. The treatments were analysed in 2 phases: at week 0 for the first phase of analysis and after 8 weeks of storage in an airtight plastic container at room temperature. The samples were then taken to the laboratory for the second phase of analysis. This study revealed that processing (oven drying, frying and smoking) of Oreochromis niloticus with ginger-garlic gave high nutritive content after 8 weeks of storage and thus might prevent the use of obnoxious substances as a means of preservation and substantially improve consumer’s satisfaction and quality fish protein intake.

Processing Solid Waste of Animal Beef using Bioactivators in Effort to Reduce Environmental Pollution

Cattle waste both in the form of solid and liquid waste that is produced every day if left alone without proper waste treatment, it can cause environmental pollution, namely air, water, and soil pollution. The environment becomes squalid and attracts various animals such as flies and dogs which can carry various diseases such as deare, typhoid, cholera and skin diseases which can spread quickly and smell bad. One of the efforts to overcome environmental pollution around cattle is to carry out a composting process using cow rumen bioactivator. This study aims: (1) to find out whether cow rumen bioactivator can reduce the unpleasant odor caused by cattle waste, (2) find out whether cow rumen bioactivator can accelerate the composting process, (3) determine the effect of bioactivator volume on compost quality, namely nitrogen content. (N-total), Phosphorus levels, and Potassium levels. This study uses qualitative analysis to test odors (H2S gas, CO2 gas, NH3 gas and CH4 gas) which was carried out at the CHEMICAL Laboratory of SMK Kesehatan Bhakti Indonesia Medika Kediri, and quantitative analysis to test Nitrogen levels using a Kjeldahl flask, Phosphorus levels with a visible spectrophotometer. , Potassium levels with AAS conducted at the Laboratory of PTPN X Jengkol Plosoklaten Kediri. This study showed: (1) after the composting process, cattle solid waste did not smell, (2) the composting process took 30 days, (3) the addition of various bioactivators (25 ml, 50 ml, 75 ml, and 100 ml), volume 50 ml is the optimal volume in the composting process with 3 kg of cattle solid waste. From the decomposition results, total nitrogen content was 0.592%, phosphorus content was 0.27%, and potassium content was 0.538%. These levels meet the Quality Standards for organic fertilizers and soil enhancers according to the Regulation of the Minister of Agriculture no. 2/Pert./HK.060/2/2006.

Impact of Wastewater on Ditches of Rainwater Drain in Al-Hilla City

In this research, the environmental reality of the ditches to rainwater drain located in the residential neighborhoods of Al-Hilla city in Iraq was studied. The study was conducted on three ditches near schools and health center within three residential neighborhoods. Chemical laboratory tests (BOD, COD, TSS, TN, TP, NO2-, NO3-, SO42-, Cl-, CN-, pH, T, DO) were proceeded on this water and it was found that the water is high polluted in sewage water compared to the sewage entering the treatment plant in the city and comparing with samples of water taken from ditches outside the city (as a standard sample). The pollution of this ditches with sewage water is considerable hazard on people health in this urban area, especially on children, students and the people come in to the health center in this areas.

Denis Papin's digester and its eighteenth-century European circulation

The digester, invented by Denis Papin in the 1680s, was a rudimentary pressure cooker used to soften hard bodies by boiling them at high pressure. In this paper, I propose a reassessment of Papin's work on the digester, arguing that his research was located at the intersection of the chemical laboratory and cooking practice. I then examine cases from the eighteenth-century European circulation of the instrument in Sweden, Italy and the Netherlands in order to showcase the different practices in which the digester was embedded, including chemical research, philanthropic projects to feed the destitute, and proposals for the improvement of home cooking. The digester's history represents a key episode for demonstrating the intertwined nature of natural-philosophical research and the practice of economy or ‘thrift’. All users of the digester engaged in a rationalization of its functions through quantification, not only to fulfil a concern for precision but also to display the device's potential to reform practical daily life. The digester could save time and fuel, reduce material waste, make cooking easier and foster collective meal preparation for the needy.

Survey of Jordanian Awareness about Hazardous Symbols of Chemicals

The use of chemical substances has commonly increased, there are such a number of chemical dangers all spherical us that it is probably almost now no longer feasible to feature if we centered constantly on the dangers. This is precisely why we need to don't forget the dangers. Everyone need to apprehend exactly what do in case of unstable contact with risky material. Previously we tested consciousness of Jordanian peoples and measured the employees’ interest of risky chemical compounds1. So this new seek aimed to research chemical symbols attentions, a questionnaire survey come to be executed among a whole of 245 peoples. The questionnaire come to be acquainted with flammable risky symbols as 90.6%, however handiest 7% for fitness chance symbol. Statistical assessment of the statistics come to be finished with the Statistical Package for Social Sciences (SPSS) version 25. The effects show that the descriptive information confirmed that scholars proven truthful to excellent familiarity and expertise of chemical chance caution symbols. Most college students had bad to truthful attitudes closer to chemical laboratory protection; however, the evaluation of college students’ chemical laboratory protection practices found out truthful to suitable practices. While college students’ protection focus and practices, however now no longer attitude, at this college have been acceptable, protection tactics want to be applied inside an extra expert protection training and coherent threat and protection weather management

Making sense of gas measurements: quantification of multicomponent gas mixtures in biological and chemical laboratory experiments

Textbooks in physical chemistry start from the treatise of the ideal gas. Gaseous compounds are important reactants and products of (bio)chemical reactions, and thus their absolute amounts are needed to establish mass balances. However, in contrast to solid, for dissolved and liquid compounds, their qualitative and especially quantitative analysis is less widely established in biological and chemical laboratories. This can be mainly ascribed to the seemingly simple chemical nature of gaseous compounds that is in contrast to the effort needed for their precise quantification. In this article, we will guide the reader through the considerations and steps needed to perform a meaningful analysis of multicomponent gas mixtures, which are reactants for or products of (bio)chemical reactions in aqueous solutions in the laboratory environment and scale. After a brief introduction, special focus is set on the methods for quantification and calculations needed to derive absolute amounts of gases in a mixture. The overall concept will be exemplified by biogas production as well as by an electroorganic reaction (Kolbe electrolysis of n-hexanoic acid), and general pitfalls will be highlighted.