Optimizing a liquid chromatographic mobile phase for simultaneous analysis of eight antiepileptic drugs.

Abstract A simple, isocratic liquid-chromatographic method was developed for simultaneously measuring ethosuximide, primidone, phenobarbital, phenytoin, carbamazepine, and their bioactive metabolites within 10 min. The chromatographic system involves a Waters' Radial-NOVA PAK C18 reversed-phase column and acetone/methanol/acetonitrile/10 mmol/L phosphate buffer (10/21/8/61 by vol, pH adjusted to 7.95 with NaOH) as mobile phase. The antiepileptic drugs are extracted from 50 microL of serum by mixing with 50 microL of acetonitrile containing 10 mg of tolybarb per liter as internal standard. After centrifugation, 20 microL of the supernate is injected onto the column and eluted with mobile phase at the rate of 2.8 mL/min at ambient temperature. The column effluent is monitored at 200 nm. The method can detect the five antiepileptic drugs in concentrations as low as 0.5 mg/L. Analytical recovery ranges from 98 to 102%. Within-run CV ranged from 2.9 to 5.8% and between-run CV from 4.7 to 7.1%. The method can also be used to measure N-desmethyl-methsuximide, chloramphenicol, and pentobarbital.

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Simultaneous analysis of acetaminophen, pseudoephedrine hydrochloride and chlorpheniramine maleate in a cold tablet using an isocratic, mixed micellar high-performance liquid chromatographic mobile phase

Journal of Chromatography A ◽

10.1016/s0021-9673(00)90050-5 ◽

1987 ◽

Vol 410 ◽

pp. 206-210 ◽

Cited By ~ 23

Author(s):

Thomas A. Biemer

Keyword(s):

Mobile Phase ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Simultaneous Analysis ◽

Chlorpheniramine Maleate ◽

Liquid Chromatographic

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A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

Current Analytical Chemistry ◽

10.2174/1573411014666180806155002 ◽

2019 ◽

Vol 15 (6) ◽

pp. 635-641

Author(s):

Nadia M. Mostafa ◽

Ghada M. Elsayed ◽

Nagiba Y. Hassan ◽

Dina A. El Mously

Keyword(s):

Simultaneous Determination ◽

Mobile Phase ◽

Dosage Form ◽

Chromatographic Method ◽

Green Analytical Chemistry ◽

Chlorpheniramine Maleate ◽

Accuracy And Precision ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

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