scholarly journals Effects of purified perforin and granzyme A from cytotoxic T lymphocytes on guinea pig ventricular myocytes

1994 ◽  
Vol 28 (5) ◽  
pp. 643-649 ◽  
Author(s):  
B. Felzen ◽  
G. Berke ◽  
D. Rosen ◽  
R. Coleman ◽  
J. Tschopp ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Guinea Pig ◽  
Cytotoxic T Lymphocytes ◽  
Ventricular Myocytes ◽  
Granzyme A

Interaction of Cytotoxic T Lymphocytes and Guinea Pig Ventricular Myocytes

1996 ◽  
Vol 78 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Bella Felzen ◽  
Revital Lavy ◽  
Maria Garcia ◽  
Gideon Berke ◽  
Phyllis Gardner ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Guinea Pig ◽  
Cytotoxic T Lymphocytes ◽  
Ventricular Myocytes

Mechanisms whereby cytotoxic T lymphocytes damage guinea-pig ventricular myocytes in vitro

1994 ◽  
Vol 427 (5-6) ◽  
pp. 422-431 ◽  
Author(s):  
Bella Felzen ◽  
Gideon Berke ◽  
Dalia Rosen ◽  
Ofer Binah
Keyword(s):  
T Lymphocytes ◽  
Guinea Pig ◽  
Cytotoxic T Lymphocytes ◽  
Ventricular Myocytes

Immunological rejection of heart transplant: how lytic granules from cytotoxic T lymphocytes damage guinea pig ventricular myocytes

1992 ◽  
Vol 420 (2) ◽  
pp. 172-179 ◽  
Author(s):  
Ofer Binah ◽  
Shimon Marom ◽  
Irit Rubinstein ◽  
Richard B. Robinson ◽  
Gideon Berke ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Guinea Pig ◽  
Cytotoxic T Lymphocytes ◽  
Heart Transplant ◽  
Ventricular Myocytes ◽  
Lytic Granules ◽  
Immunological Rejection

scholarly journals Fas (CD95/Apo-1)–Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes From Perforin-Deficient Mice

1998 ◽  
Vol 82 (4) ◽  
pp. 438-450 ◽  
Author(s):  
Bella Felzen ◽  
Mark Shilkrut ◽  
Hadar Less ◽  
Israel Sarapov ◽  
Gila Maor ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Ventricular Myocytes ◽  
Deficient Mice

scholarly journals Rab27a Is Required for Regulated Secretion in Cytotoxic T Lymphocytes

2001 ◽  
Vol 152 (4) ◽  
pp. 825-834 ◽  
Author(s):  
Jane C. Stinchcombe ◽  
Duarte C. Barral ◽  
Emilie H. Mules ◽  
Sarah Booth ◽  
Alistair N. Hume ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Immunological Synapse ◽  
Target Cells ◽  
Rab Proteins ◽  
Loss Of Function ◽  
Α Subunit ◽  
Lytic Granules ◽  
Granzyme A ◽  
Geranylgeranyl Transferase

Rab27a activity is affected in several mouse models of human disease including Griscelli (ashen mice) and Hermansky-Pudlak (gunmetal mice) syndromes. A loss of function mutation occurs in the Rab27a gene in ashen (ash), whereas in gunmetal (gm) Rab27a dysfunction is secondary to a mutation in the α subunit of Rab geranylgeranyl transferase, an enzyme required for prenylation and activation of Rabs. We show here that Rab27a is normally expressed in cytotoxic T lymphocytes (CTLs), but absent in ashen homozygotes (ash/ash). Cytotoxicity and secretion assays show that ash/ash CTLs are unable to kill target cells or to secrete granzyme A and hexosaminidase. By immunofluorescence and electron microscopy, we show polarization but no membrane docking of ash/ash lytic granules at the immunological synapse. In gunmetal CTLs, we show underprenylation and redistribution of Rab27a to the cytosol, implying reduced activity. Gunmetal CTLs show a reduced ability to kill target cells but retain the ability to secrete hexosaminidase and granzyme A. However, only some of the granules polarize to the immunological synapse, and many remain dispersed around the periphery of the CTLs. These results demonstrate that Rab27a is required in a final secretory step and that other Rab proteins also affected in gunmetal are likely to be involved in polarization of the granules to the immunological synapse.

scholarly journals Granzyme A released upon stimulation of cytotoxic T lymphocytes activates the thrombin receptor on neuronal cells and astrocytes.

1994 ◽  
Vol 91 (17) ◽  
pp. 8112-8116 ◽  
Author(s):  
H. S. Suidan ◽  
J. Bouvier ◽  
E. Schaerer ◽  
S. R. Stone ◽  
D. Monard ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Neuronal Cells ◽  
Thrombin Receptor ◽  
Granzyme A ◽  
Stimulation Of

Activity of Granzyme A, a Serine Protease in the Killing Granules of Cytotoxic T Lymphocytes, Is Reduced in Cells from HIV-infected Hemophiliacs

1996 ◽  
Vol 12 (3) ◽  
pp. 235-239 ◽  
Author(s):  
CHENG-FENG CHIANG ◽  
SCOTT A. TENENBAUM ◽  
C. REYNOLD VERRET ◽  
CINDY A. LEISSINGER ◽  
ROBERT F. GARRY
Keyword(s):  
T Lymphocytes ◽  
Serine Protease ◽  
Cytotoxic T Lymphocytes ◽  
Granzyme A ◽  
In Cells
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