Cold Sweetening in Diploid Potato: Mapping Quantitative Trait Loci and Candidate Genes

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1423-1434
Author(s):  
Cristina M Menéndez ◽  
Enrique Ritter ◽  
Ralf Schäfer-Pregl ◽  
Birgit Walkemeier ◽  
Alexandra Kalde ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Cold Storage ◽  
Quantitative Trait ◽  
Metabolic Pathways ◽  
Sugar Content ◽  
Candidate Gene Approach ◽  
Diploid Potato ◽  
Glucose Content ◽  
Gene Markers ◽  
Cold Sweetening

Abstract A candidate gene approach has been used as a first step to identify the molecular basis of quantitative trait variation in potato. Sugar content of tubers upon cold storage was the model trait chosen because the metabolic pathways involved in starch and sugar metabolism are well known and many of the genes have been cloned. Tubers of two F1 populations of diploid potato grown in six environments were evaluated for sugar content after cold storage. The populations were genotyped with RFLP, AFLP, and candidate gene markers. QTL analysis revealed that QTL for glucose, fructose, and sucrose content were located on all potato chromosomes. Most QTL for glucose content mapped to the same positions as QTL for fructose content. QTL explaining >10% of the variability for reducing sugars were located on linkage groups I, III, VII, VIII, IX, and XI. QTL consistent across populations and/or environments were identified. QTL were linked to genes encoding invertase, sucrose synthase 3, sucrose phosphate synthase, ADP-glucose pyrophosphorylase, sucrose transporter 1, and a putative sucrose sensor. The results suggest that allelic variants of enzymes operating in carbohydrate metabolic pathways contribute to the genetic variation in cold sweetening.

scholarly journals Quantitative trait loci for starch-corrected chip color after harvest, cold storage and after reconditioning mapped in diploid potato

2019 ◽  
Vol 295 (1) ◽  
pp. 209-219 ◽  
Author(s):  
Dorota Sołtys-Kalina ◽  
Katarzyna Szajko ◽  
Iwona Wasilewicz-Flis ◽  
Dariusz Mańkowski ◽  
Waldemar Marczewski ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Cold Storage ◽  
Quantitative Trait ◽  
Genetic Factors ◽  
Starch Content ◽  
Chip Color ◽  
Diploid Potato ◽  
Trait Loci ◽  
On Chip ◽  
Variance Explained

Abstract The objective of this study was to map the quantitative trait loci (QTLs) for chip color after harvest (AH), cold storage (CS) and after reconditioning (RC) in diploid potato and compare them with QTLs for starch-corrected chip color. Chip color traits AH, CS, and RC significantly correlated with tuber starch content (TSC). To limit the effect of starch content, the chip color was corrected for TSC. The QTLs for chip color (AH, CS, and RC) and the starch-corrected chip color determined with the starch content after harvest (SCAH), after cold storage (SCCS) and after reconditioning (SCRC) were compared to assess the extent of the effect of starch and the location of genetic factors underlying this effect on chip color. We detected QTLs for the AH, CS, RC and starch-corrected traits on ten potato chromosomes, confirming the polygenic nature of the traits. The QTLs with the strongest effects were detected on chromosomes I (AH, 0 cM, 11.5% of variance explained), IV (CS, 43.9 cM, 12.7%) and I (RC, 49.7 cM, 14.1%). When starch correction was applied, the QTLs with the strongest effects were revealed on chromosomes VIII (SCAH, 39.3 cM, 10.8% of variance explained), XI (SCCS, 79.5 cM, 10.9%) and IV (SCRC, 43.9 cM, 10.8%). Applying the starch correction changed the landscape of QTLs for chip color, as some QTLs became statistically insignificant, shifted or were refined, and new QTLs were detected for SCAH. The QTLs on chromosomes I and IV were significant for all traits with and without starch correction.

Mapping of candidate genes associated with late blight resistancein potato and comparison of their location with known quantitative trait loci

2008 ◽  
Vol 88 (4) ◽  
pp. 599-610 ◽  
Author(s):  
Z. D. Tian ◽  
J. Liu ◽  
L. Portal ◽  
M. Bonierbale ◽  
C. H. Xie
Keyword(s):  
Quantitative Trait Loci ◽  
Late Blight ◽  
Candidate Gene ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Gene Markers ◽  
Trait Loci ◽  
Candidate Gene Markers

Phytophthora infestans, the causal agent of late blight, threatens potato production worldwide. Many quantitative trait loci (QTL) for late blight resistance have been mapped in several potato populations. At the same time, numerous expressed sequences tags (EST) related to late blight resistance have been deposited in databases. In order to screen for putative candidate genes associated with late blight resistance, 65 candidate genes were selected for mapping and investigation of their relationship with QTL in three diploid potato populations PCC1, BCT, and PD. In total, 26 primers from the 65 selected genes that showed PCR length polymorphism were mapped on the linkage groups of three populations. Further comparison between map location of QTL and candidate gene loci indicated that three candidate gene markers were placed in a QTL region. The locus of a putative receptor-like protein kinase b co-localized with an important QTL region on chromosome XI of PCC1. In the PD population, the Lox gene was in a QTL with moderate effect on chromosome III and two protein phosphatase loci were localized in a QTL with the largest effect on chromosome XII. These mapped candidate gene markers could be used as a bridge to other genetic maps of potato. The association of candidate genes with QTL forms the basis for further studies on the contributions of these candidate genes to natural variation for potato late blight resistance. Key words: Candidate gene, quantitative resistance loci, late blight, potato

scholarly journals Isolation and Manipulation of Quantitative Trait Loci for Disease Resistance in Rice Using a Candidate Gene Approach

2008 ◽  
Vol 1 (5) ◽  
pp. 786-793 ◽  
Author(s):  
Ke-Ming Hu ◽  
De-Yun Qiu ◽  
Xiang-Ling Shen ◽  
Xiang-Hua Li ◽  
Shi-Ping Wang
Keyword(s):  
Disease Resistance ◽  
Quantitative Trait Loci ◽  
Candidate Gene ◽  
Quantitative Trait ◽  
Candidate Gene Approach ◽  
Trait Loci

Development of the sugar beet quality in the Netherlands since 1980 and introduction of invert sugar as a new parameter for beet quality assessment

2015 ◽  
pp. 95-103 ◽  
Author(s):  
Dirk P. Vermeulen
Keyword(s):  
The Netherlands ◽  
Sugar Beet ◽  
Quality Assessment ◽  
Sugar Content ◽  
Quality Parameters ◽  
Invert Sugar ◽  
Internal Quality ◽  
Glucose Content ◽  
Thick Juice ◽  
Trial Phase

The technological beet quality has been always important for the processors of sugar beet. An investigation into the development of the beet quality in the Netherlands since 1980 has shown that beet quality has improved significantly. Internal quality parameters that are traditionally determined in the beet laboratory, i.e. sugar content, Na, K and -aminoN, all show an improving trend over the years. In the factories, better beet quality has led to lower lime consumption in the juice purification and significantly higher thick juice purity. In 2013, Suiker Unie introduced the serial analysis of the glucose content in beet brei as part of the routine quality assessment of the beet. The invert sugar content is subsequently calculated from glucose content with a new correlation. The background, the trial phase and the first experiences with the glucose analyzer are discussed.

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