Epidemiology of Clostridium difficile Colonization in Newborns: Results Using a Bacteriophage and Bacteriocin Typing System

1988 ◽  
Vol 158 (2) ◽  
pp. 349-354 ◽  
Author(s):  
A. E. Bacon ◽  
R. Fekety ◽  
D. R. Schaberg ◽  
R. G. Faix
Keyword(s):  
Clostridium Difficile ◽  
Typing System ◽  
Bacteriocin Typing

Development and application of a multiple typing system for Clostridium difficile.

1991 ◽  
Vol 57 (7) ◽  
pp. 1873-1879 ◽  
Author(s):  
D E Mahony ◽  
J Clow ◽  
L Atkinson ◽  
N Vakharia ◽  
W F Schlech
Keyword(s):  
Clostridium Difficile ◽  
Typing System

scholarly journals Typing of Clostridium difficile isolates endemic in Japan by sequencing of slpA and its application to direct typing

2010 ◽  
Vol 59 (5) ◽  
pp. 556-562 ◽  
Author(s):  
Haru Kato ◽  
Hideaki Kato ◽  
Yoichiro Ito ◽  
Takayuki Akahane ◽  
Sayuri Izumida ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Protein A ◽  
Sequence Type ◽  
Typing System ◽  
Pcr Ribotyping ◽  
Stool Specimens ◽  
Encoding Gene ◽  
Ribotype 078 ◽  
Toxic Culture ◽  
Pcr Ribotype 027

A typing system for Clostridium difficile using sequencing of the surface-layer protein A encoding gene (slpA) was evaluated and used to analyse clinical isolates in Japan. A total of 160 stool specimens from symptomatic patients in Japan was examined and 87 C. difficile isolates were recovered. slpA sequence typing was found to have reliable typability and discriminatory power in comparison with PCR ribotyping, and the typing results were highly reproducible and comparable. slpA sequence typing was used to type C. difficile in DNA extracted directly from stool specimens. Among the 90 stool specimens in which direct typing results were obtained, 77 specimens were positive for C. difficile culture, and typing results from isolated strains agreed with those from direct typing in all 77 specimens. The slpA sequence type smz was dominant at all four hospitals examined, and this endemic type was detected by culture and/or direct typing in 61 (62 %) of 99 stool specimens positive for toxic culture and/or direct slpA sequence typing. Comparison of epidemic strains reported throughout the world revealed one isolate identified as slpA sequence type gc8, which was found to correspond to PCR ribotype 027 (BI/NAP1/027), whereas no isolates were found with the slpA gene identical to that of PCR ribotype 078 strain. slpA sequence typing is valuable for comparison of C. difficile strains epidemic in diverse areas because the typing results are reproducible and can easily be shared. In addition, slpA sequence typing could be applied to direct typing without culture.

Rapid analysis of Clostridium difficile strains recovered from hospitalized patients by using the slpA sequence typing system

2009 ◽  
Vol 15 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Hideaki Kato ◽  
Makoto Nakamura ◽  
Hideaki Kato ◽  
Yasuhito Iwashima ◽  
Atsushi Nakamura ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Rapid Analysis ◽  
Hospitalized Patients ◽  
Typing System

scholarly journals A study on bacteriocin typing of avian strains ofPasteurella multocida

1980 ◽  
Vol 85 (1) ◽  
pp. 59-63
Author(s):  
Rose Mushin
Keyword(s):  
Pasteurella Multocida ◽  
Typing System ◽  
Avian Origin ◽  
Bacteriocin Typing ◽  
Set Up

SummaryIn two groups, each containing 56Pasteurella multocidastrains of avian origin, the percentage of multicin-producing strains was 80·4 and 71·4. In two groups containing 46 and 58 isolates tested for sensitivity to multicins, 82·6% and 62·1% respectively were listed as indicators.P. multocidastrains producing bacteriocins were active on a range of 1–33 indicators. A preliminary multicin typing system was set up with the use of eight selected indicators; 52% of multicin-producing strains acted on one indicator only.P. multocidaserotypes and serologically un-typable strains were subdivided into multicin types.

scholarly journals Bacteriophage and bacteriocin typing scheme for Clostridium difficile.

1983 ◽  
Vol 17 (6) ◽  
pp. 1148-1152 ◽  
Author(s):  
T L Sell ◽  
D R Schaberg ◽  
F R Fekety
Keyword(s):  
Clostridium Difficile ◽  
Typing Scheme ◽  
Bacteriocin Typing

scholarly journals Bacteriocin typing of streptococcal isolates from endocarditis

1977 ◽  
Vol 5 (5) ◽  
pp. 517-520
Author(s):  
G W Upton ◽  
H P Dalton ◽  
R A Kolstad
Keyword(s):  
Yeast Extract ◽  
Brain Heart Infusion ◽  
Clinical Situation ◽  
Bacteriocin Production ◽  
Streptococcus Sanguis ◽  
Extract Agar ◽  
Bacteriocin Producer ◽  
Typing System ◽  
Bacteriocin Typing ◽  
Yeast Extract Agar

A typing system based on bacteriocin production and sensitivity for Streptococcus sanguis strains was devised. Bacteriocin producer strains were grown (37degrees C) anaerobically on brain heart infusion-yeast extract agar for 18 h. Bacteriocin indicator strains were suspended to molten brain heart infusion-yeast extract agar and then overlayed onto the producer strain. After an additional 18 to 24 h of incubation, zones of bacteriocin inhibition were recorded. After establishment of the typing system, eight Streptococcus strains from bacterial endocarditis patients were characterized by the typing system. Four patient strains had identical bacteriocin reduction patterns but could be differentiated by differences in their bacteriocin sensitivity. Two isolates from the same patient had identical production and sensitivity patterns. By including both bacteriocin production and bacteriocin sensitivity, all seven epidemiologically unrelated isolates could be differentiated. On the basis of these results, there does not appear to be a correlation between bacteriocin production and infectivity, but the usefulness of the application of a typing system to a clinical situation is demonstrated.

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