Expression and Characterization of a cDNA Clone Encoding an Immunodominant Surface Glycoprotein of Pneumocystis carinii

1992 ◽  
Vol 166 (5) ◽  
pp. 1113-1123 ◽  
Author(s):  
P. J. Haidaris ◽  
T. W. Wright ◽  
F. Gigliotti ◽  
C. G. Haidaris
Keyword(s):  
Cdna Clone ◽  
Pneumocystis Carinii ◽  
Surface Glycoprotein

scholarly journals Characterization of Major Surface Glycoprotein Genes of Human Pneumocystis carinii and High-Level Expression of a Conserved Region

1998 ◽  
Vol 66 (9) ◽  
pp. 4268-4273 ◽  
Author(s):  
Qin Mei ◽  
Ross E. Turner ◽  
Vivian Sorial ◽  
Diane Klivington ◽  
C. William Angus ◽  
...  
Keyword(s):  
Genomic Library ◽  
Pneumocystis Carinii ◽  
Surface Glycoprotein ◽  
High Level Expression ◽  
Serum Samples ◽  
Major Surface Glycoprotein ◽  
Adult Humans ◽  
Major Surface ◽  
High Level

ABSTRACT To facilitate studies of Pneumocystis cariniiinfection in humans, we undertook to better characterize and to express the major surface glycoprotein (MSG) of human P. carinii, an important protein in host-pathogen interactions. Seven MSG genes were cloned from a single isolate by PCR or genomic library screening and were sequenced. The predicted proteins, like rat MSGs, were closely related but unique variants, with a high level of conservation among cysteine residues. A conserved immunodominant region (of approximately 100 amino acids) near the carboxy terminus was expressed at high levels in Escherichia coli and used in Western blot studies. All 49 of the serum samples, which were taken from healthy controls as well as from patients with and withoutP. carinii pneumonia, were reactive with this peptide by Western blotting, supporting the hypothesis that most adult humans have been infected with P. carinii at some point. This recombinant MSG fragment, which is the first human P. carinii antigen available in large quantities, may be a useful reagent for investigating the epidemiology of P. cariniiinfection in humans.

Characterization of Major Surface Glycoprotein Genes of Human Pneumocystis carinii and High-Level Expression of a Conserved Region

1998 ◽  
Vol 66 (9) ◽  
pp. 4268-4273
Author(s):  
Qin Mei ◽  
Ross E. Turner ◽  
Vivian Sorial ◽  
Diane Klivington ◽  
C. William Angus ◽  
...  
Keyword(s):  
Pneumocystis Carinii ◽  
Surface Glycoprotein ◽  
High Level Expression ◽  
Major Surface Glycoprotein ◽  
Conserved Region ◽  
Major Surface ◽  
High Level

scholarly journals The cloning and characterization of the arom gene of Pneumocystis carinii

1993 ◽  
Vol 139 (12) ◽  
pp. 2901-2914 ◽  
Author(s):  
S. Banerji ◽  
A. E. Wakefield ◽  
A. G. Allen ◽  
D. J. Maskell ◽  
S. E. Peters ◽  
...  
Keyword(s):  
Pneumocystis Carinii

Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase

1993 ◽  
Vol 23 (3) ◽  
pp. 543-551 ◽  
Author(s):  
Cathal Wilson ◽  
Norbert Eller ◽  
Anton Gartner ◽  
Oscar Vicente ◽  
Erwin Heberle-Bors
Keyword(s):  
Map Kinase ◽  
Cdna Clone ◽  
Isolation And Characterization
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