In-vitro susceptibility of Gram-positive cocci to LY146032 teicoplanin, sodium fusidate, vancomycin, and rifampicin

1987 ◽  
Vol 20 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Donald J. Pohlod ◽  
Louis D. Saravolatz ◽  
Margaret M. Somerville
Keyword(s):  
Gram Positive ◽  
In Vitro Susceptibility ◽  
Gram Positive Cocci

scholarly journals In vitro susceptibility of gram-positive cocci to paldimycin.

1987 ◽  
Vol 31 (1) ◽  
pp. 104-107 ◽  
Author(s):  
D J Pohlod ◽  
L D Saravolatz ◽  
M M Somerville
Keyword(s):  
Gram Positive ◽  
In Vitro Susceptibility ◽  
Gram Positive Cocci

Use of Fosfomycin Etest® to Determine In Vitro Susceptibility of Clinical Isolates of Enterobacterales Other Than Escherichia coli , Non-Fermenting Gram-Negative Bacilli, and Gram-positive Cocci

2021 ◽  
Author(s):  
James A. Karlowsky ◽  
Melanie R. Baxter ◽  
Alyssa R. Golden ◽  
Heather J. Adam ◽  
Andrew Walkty ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolates ◽  
High Rate ◽  
Major Error ◽  
Gram Positive ◽  
In Vitro Susceptibility ◽  
Gram Negative ◽  
Agar Dilution ◽  
Gram Positive Cocci

Clinical isolates of Enterobacterales other than Escherichia coli (EOTEC), non-fermenting Gram-negative bacilli, and Gram-positive cocci were tested for susceptibility to fosfomycin using Etest ® and reference agar dilution. Applying EUCAST (v. 11.0, 2021) intravenous fosfomycin breakpoints, Etest ® MICs for EOTEC showed essential agreement (EA), categorical agreement (CA), major error (ME), and very major error (VME) rates of 70.4%, 88.4%, 4.1%, and 32.1%, respectively. No species of EOTEC tested with acceptable rates for all of EA (≥90%), CA (≥90%), ME (≤3%), and VME (≤3%). Etest ® MICs for Enterococcus faecalis , interpreted using CLSI oral/urine criteria (M100, 2021), showed EA, CA, minor error, ME, and VME rates of 98.5%, 81.2%, 18.8%, 0%, and 0%. Against Staphylococcus aureus , EA, CA, and ME rates were 84.1%, 98.7%, and 1.3% (EUCAST intravenous criteria). S. aureus isolates with fosfomycin MICs >32 μg/ml (resistant) were not identified by agar dilution. We conclude performing fosfomycin Etest ® on isolates of S. aureus will reliably identify fosfomycin-susceptible isolates with low, acceptable rates of MEs and VMEs. Testing of urinary isolates of E. faecalis by Etest ® is associated with an unacceptably high rate of minor errors (18.8%) but low, acceptable rates of MEs and VMEs when results are interpreted using CLSI criteria. Isolates of EOTEC tested by Etest ® with resulting MICs interpreted by EUCAST criteria were associated with an unacceptably high VME rate (32.1%). In vitro testing of clinical isolates beyond E. coli , E. faecalis , and S. aureus to determine susceptibility to fosfomycin is problematic with current methods and breakpoints.

In vitro activity of telavancin and comparators against selected groups of Gram-positive cocci

2013 ◽  
Vol 41 (3) ◽  
pp. 213-217 ◽  
Author(s):  
Russell Hope ◽  
Aiysha Chaudhry ◽  
Rachael Adkin ◽  
David M. Livermore
Keyword(s):  
Vitro Activity ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Positive Cocci

Comparative in vitro activity of ceftobiprole against Gram-positive cocci

2010 ◽  
Vol 36 (2) ◽  
pp. 111-113 ◽  
Author(s):  
Carmen Betriu ◽  
Esther Culebras ◽  
María Gómez ◽  
Fátima López-Fabal ◽  
Iciar Rodríguez-Avial ◽  
...  
Keyword(s):  
Vitro Activity ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Positive Cocci

Działanie in vitro olejku cyprysowego (Oleum Cupressi) na bakterie beztlenowe

2019 ◽  
Vol 20 (2) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Anaerobic Bacteria ◽  
Growth Control ◽  
Cupressus Sempervirens ◽  
Tannerella Forsythia ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Gram Positive Cocci ◽  
Porphyromonas Levii

Introduction. Cypress (Cupressus sempervirens L.) belongs to the family Cupressaceae. It is evergreen, and grows in Mediterranean region. The Cypress leaves and young branches are utilized to produce the essential oil. Cypress oil contain a number of components, in it α-pinene, Δ3-carene, α-terpinyl acetate, cedrol, α-terpinolene, β-myrcene, limonene, α-terpineolene, terpinen-4-ol, β-pinene, δ-cadinene and sabinene. The oil is used in therapy different diseases. It to have antimicrobial activity. Aim. The aim of the date was evaluation the susceptibility of anaerobic bacteria to Cypress oil. Material and methods. The anaerobic bacteria were isolated from patients. The 62 microorganisms, in it 36 strains of Gram-negative rods, 14 Gram-positive cocci and 12 Gram-positive rods, and 7 reference strains were tested. Susceptibility (MIC) was determined by means of plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. The Cypress oil was dissolved in DMSO and distilled water to obtain final following concentrations: 2.5, 5.0, 7.5, 10.0, 15.0 and 20.0 mg/ml. Inoculum containing 106 CFU per 1 ml was seeded with Steers replicator upon the agar with oil or without the oil (strains growth control). The agar plates was incubated in anaerobic condition in anaerobic jar in 37°C for 48 hrs. The MIC was interpreted as the lowest concentration of Cypress oil inhibiting the growth of tested bacteria. Results. The results indicated that from among Gram-negative rods the most susceptible to Cypress oil was the strains from genus Tannerella forsythia (MIC < 2.5-5.0 mg/ml), Bacteroides uniformis (MIC = 5.0 mg/ml), Bacteroides vulgatus and Porphyromonas asaccharolytica (MIC 5.0-7.5 mg/ml) and Porphyromonas levii (MIC = 7.5 mg/ml). The strains from genera Fusobacterium and of Bacteroides fragilis were the susceptible to 2.5-≥ 20.0 mg/ml. The Cypress oil was least active towards Prevotella and Parabacteroides strains (MIC ≥ 20.0 mg/ml).The tested Gram-positive cocci were more susceptible. The growth of the strains were inhibited by concentrations in ranges ≤ 2.5-7.5 mg/ml. The oil was minor active towards Gram-positive rods (MIC ≤ 2.5-20.0 mg/ml). Among the strains the genus of Actinomyces odontolyticus (MIC = 5.0 mg/ml) and Actinomyces viscosus (MIC ≤ 2.5-7.5 mg/ml) were the most susceptible. The growth of rods of Bifidobacterium breve was inhibited by concentrations 10.0 mg/ml. The data indicates that the Gram-negative rods were the less susceptible than Gram-positive bacteria to cypress oil. Conclusions. Among Gram-negative rods the most susceptible were the strains Tannerella forsythia, Bacteroides uniformis, Bacteroides vulgatus, Porphyromonas asaccharolytica and Porphyromonas levii. The oil was more active against Gram-positive cocci. Gram-positive anaerobic bacteria demonstrate the more susceptible to Cypress oil then Gram-positive rods.

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