scholarly journals Altered sterol profile induced in Leishmania amazonensis by a natural dihydroxymethoxylated chalcone

2009 ◽  
Vol 63 (3) ◽  
pp. 469-472 ◽  
Author(s):  
E. C. Torres-Santos ◽  
M. I. Sampaio-Santos ◽  
F. S. Buckner ◽  
K. Yokoyama ◽  
M. Gelb ◽  
...  
2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Valter Viana Andrade-Neto ◽  
Thaís Martins Pereira ◽  
Marilene do Canto-Cavalheiro ◽  
Eduardo Caio Torres-Santos

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
MA Brenzan ◽  
CV Nakamura ◽  
BPD Filho ◽  
T Ueda-Nakamura ◽  
MCM Young ◽  
...  

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
LS Espindola ◽  
EM Silva ◽  
RM Araújo ◽  
R Braz-Filho

2021 ◽  
Vol 9 (2) ◽  
pp. 320
Author(s):  
Wilmer Alcazar ◽  
Sami Alakurtti ◽  
Maritza Padrón-Nieves ◽  
Maija Liisa Tuononen ◽  
Noris Rodríguez ◽  
...  

Herein, we evaluated in vitro the anti-leishmanial activity of betulin derivatives in Venezuelan isolates of Leishmania amazonensis, isolated from patients with therapeutic failure. Methods: We analyzed promastigote in vitro susceptibility as well as the cytotoxicity and selectivity of the evaluated compounds. Additionally, the activity of selected compounds was determined in intracellular amastigotes. Finally, to gain hints on their potential mechanism of action, the effect of the most promising compounds on plasma and mitochondrial membrane potential, and nitric oxide and superoxide production by infected macrophages was determined. Results: From the tested 28 compounds, those numbered 18 and 22 were chosen for additional studies. Both 18 and 22 were active (GI50 ≤ 2 µM, cytotoxic CC50 > 45 µM, SI > 20) for the reference strain LTB0016 and for patient isolates. The results suggest that 18 significantly depolarized the plasma membrane potential (p < 0.05) and the mitochondrial membrane potential (p < 0.05) when compared to untreated cells. Although neither 18 nor 22 induced nitric oxide production in infected macrophages, 18 induced superoxide production in infected macrophages. Conclusion: Our results suggest that due to their efficacy and selectivity against intracellular parasites and the potential mechanisms underlying their leishmanicidal effect, the compounds 18 and 22 could be used as tools for designing new chemotherapies against leishmaniasis.


Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07136
Author(s):  
Ma. Florencia Peralta ◽  
Ma. Laura Guzman ◽  
Ma. Estefanía Bracamonte ◽  
J. Diego Marco ◽  
Ma. Eugenia Olivera ◽  
...  

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