Collaborative Study of a New Method for the Extraction of Light Filth from Soy Flour

1972 ◽  
Vol 55 (1) ◽  
pp. 60-61
Author(s):  
Russell G Dent ◽  
Michael Brunson
Keyword(s):  
Petroleum Ether ◽  
Collaborative Study ◽  
Enzymatic Digestion ◽  
Mineral Oil ◽  
New Method ◽  
Soy Flour ◽  
Wet Sieving ◽  
Official Procedure

Abstract Collaborative results are presented for a proposed method for light filth extraction from soy flour. The method involves dispersion in saturated NaCl and isopropanol, wet sieving, and extraction with hot mineral oil from alcohol in a percolator. Average per cent recoveries of spiked elytral fragments and rodent hairs were 88.2 and 65.5, respectively, by the proposed method compared to 64.5 and 56, respectively, by official procedure 40.053. The existing procedure for soy flour is time-consuming because it requires an overnight enzymatic digestion and a hazardous petroleum ether defatting step. The proposed method eliminates both of these steps arid the analysis can be completed in 1–2 hr. It is recommended that the method be adopted as official first action to replace 40.053.

Extraction of Light Filth from Barley, Oatmeal, and Mixed Dry Infant Cereals: Collaborative Study

1980 ◽  
Vol 63 (2) ◽  
pp. 187-188
Author(s):  
Russell G Dent ◽  
◽  
D M Floyd ◽  
J Nagy ◽  
A R Olsen ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
New Method ◽  
Interlaboratory Studies ◽  
Light Mineral ◽  
Wet Sieving

Abstract A new method has been developed for the extraction of light filth from oatmeal, barley, and mixed dry infant cereals which involves HC1 digestion followed by wet sieving. The residue is defatted in a paper cup, using isopropanol, and transferred to a 2 L Wildman trap flask with 40% isopropanol. Filth elements are extracted with light mineral oil. Interlaboratory studies resulted in 92 and 86% average recoveries of rodent hairs and insect fragments, respectively. The proposed method has been adopted as official first action.

Collaborative Study of an Enzymatic Digestion Method for the Isolation of Light Filth from Ground Beef or Hamburger

1976 ◽  
Vol 59 (1) ◽  
pp. 51-52
Author(s):  
Phillip Alioto ◽  
Mary Andreas
Keyword(s):  
Collaborative Study ◽  
Ground Beef ◽  
Enzymatic Digestion ◽  
Mineral Oil ◽  
Digestion Method ◽  
Light Mineral ◽  
Filter Papers ◽  
Wet Sieving

Abstract Collaborative results are presented for a proposed method for light filth extraction from ground beef or hamburger. The method involves enzymatic digestion, wet sieving, and extraction with light mineral oil from 40% isopropanol. Recoveries are good and filter papers are clean. This method has been adopted as official first action.

Extraction of Light Filth from Fish Paste and Sauce (Bagoong) Not Containing Spice: Collaborative Study

1992 ◽  
Vol 75 (2) ◽  
pp. 263-265
Author(s):  
Larry E Glaze
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
New Method ◽  
Test Portion ◽  
Plain Weave ◽  
Wet Sieving

Abstract A collaborative study was conducted to validate a new method for the extraction of light filth from fish paste and sauce (Bagoong) not containing spice. A 225 g test portion is digested by boiling in a mixture of acid and emulsifying agents. Light filth is isolated by wet sieving on a No. 230 plain weave sieve with Tergitol, a deaeration boil in 40% isopropanol, and flotation with mineral oil and 40% isopropanol in a Wildman trap flask. Three spiking levels were used in the study for rat hairs and Insect fragments; 1 level was used for whole or equivalent insects. For rat hairs, recoveries at the low, medium, and high levels averaged 77,94, and 76%, respectively. Recoveries of Insect fragments for these levels averaged 92,88, and 93%, respectively; recoveries of whole or equivalent Insects averaged 85,70, and 80%, respectively. The method was adopted first action by AOAC International for the extraction of light filth from fish paste and sauce (Bagoong) not containing spice.

Collaborative Study of an Enzymatic Digestion Method for the Isolation of Light Filth from Fresh Pork Sausage

1973 ◽  
Vol 56 (3) ◽  
pp. 631-633
Author(s):  
John S Gecan ◽  
Philip Alioto
Keyword(s):  
Particle Size ◽  
Collaborative Study ◽  
Enzymatic Digestion ◽  
Mineral Oil ◽  
Size Reduction ◽  
Particle Size Reduction ◽  
Digestion Method ◽  
Pork Sausage ◽  
Wet Sieving ◽  
Better Than

Abstract An alternative method has been developed for the isolation of light filth from fresh pork sausage. The method involves particle size reduction, enzymatic digestion, wet sieving, deaerating, and floating with n-heptane-mineral oil from cooled 40% isopropanol. Collaborative study of the method showed the extraction papers and the recovery of light filth elements to be comparable to or better than those obtained for 40.B07. The method has been adopted as official first action as an alternative to 40.B07.

Collaborative Study of an Acid Autoclave Method for Mineral Oil Extraction of Light Filth from Spaghetti and Macaroni

1969 ◽  
Vol 52 (3) ◽  
pp. 463-465
Author(s):  
Joel J Thrasher
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
Oil Extraction ◽  
New Method ◽  
Official Method ◽  
Plain Weave ◽  
Filter Papers ◽  
Wet Sieving ◽  
Better Than

Abstract A method for the extraction of light filth from alimentary pastes based on rapid autoclaving of acidified samples, wet sieving on a No. 230 plain weave sieve, and heating the sieve retainings in an acidic mineral oil mixture is described. This new method is faster and results in higher and more reproducible recoveries than method 36.025. Recoveries were equal or better than those achieved by the official method, and the filter papers were cleaner.

Collaborative Study of a New Method for the Extraction of Light Filth from White Flour

1972 ◽  
Vol 55 (3) ◽  
pp. 514-515
Author(s):  
Mary T Miller
Keyword(s):  
Acid Hydrolysis ◽  
Collaborative Study ◽  
Mineral Oil ◽  
New Method ◽  
White Flour

Abstract A new method for the separation of light filth from white flour is based on an autoclave-acid hydrolysis, followed by separation of light fdth elements with mineral oil in a Corning or Kilborn separator. This method is rapid and gives clean papers and good recoveries. The method has been adopted as official first action, to replace 40.045 and 40.046 for extraction of light filth from white flour.

Extraction of Light Filth from Whole Peppermint Leaves: Collaborative Study

1985 ◽  
Vol 68 (4) ◽  
pp. 697-698
Author(s):  
Jack Boese ◽  
Marvin Nakashima ◽  
Larry E Glaze
Keyword(s):  
Collaborative Study ◽  
Tween 80 ◽  
Mineral Oil ◽  
Isopropanol Solution ◽  
Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from whole peppermint leaves. A 5 g sample is defatted with isopropanol in a simple reflux appartus. Rat hairs, insect fragments, and whole insects are isolated by wet sieving on a No. 230 sieve, a deaerating boil in 40% isopropanol solution, flotation with Tween 80-Na4edta (1 + 1) and mineral oil-heptane (85 + 15), and trappings in a Wildman trap flask. Average recoveries obtained by 6 collaborators for 3 spike levels of rat hairs (5,10,15) were 83.3, 87.5, and 82.2%, respectively. For whole insects (5,10,15) recoveries averaged 85.0, 80.0 and 77.2% respectively; for insect fragments (20, 30, 50) recoveries averaged 79.6, 88.3, and 84.8%, respectively. The average recoveries for the 3 levels of each analyte were not significantly different. The method has been adopted official first action.

Collaborative Study of a New Method for Extraction of Light Filth from Reconditioned (Coarsely Ground) Nutmeg

1971 ◽  
Vol 54 (3) ◽  
pp. 575-578
Author(s):  
Alberto W Vazquez
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
New Method ◽  
Official Method ◽  
Collaborative Testing

Abstract A new method has been developed for extraction of light filth from coarsely ground nutmeg. Nutmeg tissue is defatted by 3 successive 10 min extractions with boiling CHCI3, after which the solvent is removed by aspiration and an isopropanol rinse. Following sieving on a No. 230 sieve and a brief deaeration and hydrolysis step in acidified boiling 60% ethanol-CaCl2, light filth elements are extracted with mineral oil from the 60% ethanol-CaCl2 mixture. Collaborative testing of the proposed method yielded satisfactory recoveries of added light filth elements. The official method for ground spices, 40.106(b), was compared in a collaborative study with the proposed method for the analysis of ground nutmeg and mace. Results were encouraging, but further study is indicated. The method as described for reconditioned nutmeg has been adopted as official first action.

Extraction of Light Filth from Oriental Sauces Containing Soy Sauce, Thickeners, and Spices: Collaborative Study

1993 ◽  
Vol 76 (1) ◽  
pp. 47-49
Author(s):  
Marvin J Nakashima ◽  
◽  
J A Gallman ◽  
R R Haynos ◽  
C E Highfield ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Steam Bath ◽  
Mineral Oil ◽  
Soy Sauce ◽  
Test Portion ◽  
Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from oriental sauces containing soy sauce, thickeners, and spices. A100 g test portion is pretreated in a 2% solution of Tergitol Anionic 4 over a steam bath, and oils are removed by wet-sieving on No. 230 sieve. Filth is isolated from 40% isopropanol by using Na4EDTA and mineral oil. Average recoveries by 9 collaborators for 3 spike levels of rat hairs (5,10, and 15) were 84, 78, and 79%, respectively; for insect fragments (5,15, and 30), recoveries were 92, 95, and 96%, respectively. The method was adopted first action by AOAC International.

Collaborative Study of a Method for the Extraction of Light Filth from Ground Mace and Ground Caraway Seed

1976 ◽  
Vol 59 (4) ◽  
pp. 827-829
Author(s):  
Joel J Thrasher
Keyword(s):  
Collaborative Study ◽  
Mineral Oil ◽  
New Method ◽  
Filter Papers

Abstract A new method has been developed for the extraction of light filth from ground mace and ground caraway seed. The method uses a 2-step chloroform-isopropanol pretreatment followed by separation of the light filth from 40% isopropanol with mineral oil. The collaborative study resulted in clean filter papers with more reproducible recoveries of light filth elements than the official first action method, 44.116(b). The method has been adopted as official first action.

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