Extraction of Light Filth from Fish Paste and Sauce (Bagoong)
Not Containing Spice: Collaborative Study

Abstract A collaborative study was conducted to validate a new method for the extraction of light filth from fish paste and sauce (Bagoong) not containing spice. A 225 g test portion is digested by boiling in a mixture of acid and emulsifying agents. Light filth is isolated by wet sieving on a No. 230 plain weave sieve with Tergitol, a deaeration boil in 40% isopropanol, and flotation with mineral oil and 40% isopropanol in a Wildman trap flask. Three spiking levels were used in the study for rat hairs and Insect fragments; 1 level was used for whole or equivalent insects. For rat hairs, recoveries at the low, medium, and high levels averaged 77,94, and 76%, respectively. Recoveries of Insect fragments for these levels averaged 92,88, and 93%, respectively; recoveries of whole or equivalent Insects averaged 85,70, and 80%, respectively. The method was adopted first action by AOAC International for the extraction of light filth from fish paste and sauce (Bagoong) not containing spice.

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Collaborative Study of an Acid Autoclave Method for Mineral Oil Extraction of Light Filth from Spaghetti and Macaroni

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/52.3.463 ◽

1969 ◽

Vol 52 (3) ◽

pp. 463-465

Author(s):

Joel J Thrasher

Keyword(s):

Collaborative Study ◽

Mineral Oil ◽

Oil Extraction ◽

New Method ◽

Official Method ◽

Plain Weave ◽

Filter Papers ◽

Wet Sieving ◽

Better Than

Abstract A method for the extraction of light filth from alimentary pastes based on rapid autoclaving of acidified samples, wet sieving on a No. 230 plain weave sieve, and heating the sieve retainings in an acidic mineral oil mixture is described. This new method is faster and results in higher and more reproducible recoveries than method 36.025. Recoveries were equal or better than those achieved by the official method, and the filter papers were cleaner.

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Extraction of Light Filth from Oriental Fish Products Containing Spice: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/76.1.44 ◽

1993 ◽

Vol 76 (1) ◽

pp. 44-46

Author(s):

Larry E Glaze ◽

◽

W Davis ◽

D M Floyd ◽

C R Graham ◽

...

Keyword(s):

Collaborative Study ◽

Mineral Oil ◽

New Method ◽

Test Portion ◽

Fish Products ◽

Plain Weave

Abstract A collaborative study was conducted to validate a new method for the extraction of light filth from oriental fish products containing spice. A100 g test portion is digested by boiling in a mixture of HCI, Igepal DM-710, and CO-730. Light filth is isolated by wetsieving on a No. 230 plain-weave sieve with Tergitol, deaeration boiling in 40% isopropanol, and extracting with mineral oil-heptane (85 + 15) and 40% isopropanol in a Wildman trap flask. Three spiking levels for rat hairs and insect fragments were used in the study. For rat hairs, recoveries at the low, medium, and high levels averaged 80.0,71.6, and 88.0%, respectively. Recoveries of insect fragments for low, medium, and high levels averaged 87.8,83.7, and 89.4%, respectively. The method was adopted first action by AOAC International.

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Extraction of Light Filth from Oriental Sauces Containing Soy Sauce, Thickeners, and Spices: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/76.1.47 ◽

1993 ◽

Vol 76 (1) ◽

pp. 47-49

Author(s):

Marvin J Nakashima ◽

◽

J A Gallman ◽

R R Haynos ◽

C E Highfield ◽

...

Keyword(s):

Collaborative Study ◽

Steam Bath ◽

Mineral Oil ◽

Soy Sauce ◽

Test Portion ◽

Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from oriental sauces containing soy sauce, thickeners, and spices. A100 g test portion is pretreated in a 2% solution of Tergitol Anionic 4 over a steam bath, and oils are removed by wet-sieving on No. 230 sieve. Filth is isolated from 40% isopropanol by using Na4EDTA and mineral oil. Average recoveries by 9 collaborators for 3 spike levels of rat hairs (5,10, and 15) were 84, 78, and 79%, respectively; for insect fragments (5,15, and 30), recoveries were 92, 95, and 96%, respectively. The method was adopted first action by AOAC International.

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Extraction of Light Filth from Barley, Oatmeal, and Mixed Dry Infant Cereals: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/63.2.187 ◽

1980 ◽

Vol 63 (2) ◽

pp. 187-188

Author(s):

Russell G Dent ◽

◽

D M Floyd ◽

J Nagy ◽

A R Olsen ◽

...

Keyword(s):

Collaborative Study ◽

Mineral Oil ◽

New Method ◽

Interlaboratory Studies ◽

Light Mineral ◽

Wet Sieving

Abstract A new method has been developed for the extraction of light filth from oatmeal, barley, and mixed dry infant cereals which involves HC1 digestion followed by wet sieving. The residue is defatted in a paper cup, using isopropanol, and transferred to a 2 L Wildman trap flask with 40% isopropanol. Filth elements are extracted with light mineral oil. Interlaboratory studies resulted in 92 and 86% average recoveries of rodent hairs and insect fragments, respectively. The proposed method has been adopted as official first action.

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Extraction of Light Filth from Whole Leaves of Alfalfa, Lemon Balm, Papaya, and Spearmint: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/70.6.997 ◽

1987 ◽

Vol 70 (6) ◽

pp. 997-999

Author(s):

Marvin J Nakashima ◽

Larry E Glaze

Keyword(s):

Collaborative Study ◽

Tween 80 ◽

Mineral Oil ◽

Test Portion ◽

Lemon Balm ◽

Aoac Method ◽

Wet Sieving

Abstract Results are reported for a collaborative study to extend AOAC method 44.A06-44.A08 to extraction of light filth from whole leaves of alfalfa, lemon balm, papaya, and spearmint. A 5 g (spearmint) or 10 g (alfalfa, lemon balm, papaya) test portion is defatted with isopropanol in a simple reflux apparatus. Rat hairs, insect fragments, and whole insects are isolated by wet sieving on a No. 230 sieve, a deaerating boil in 40% isopropanol, and flotation with mineral oil-heptane (85 + 15) from Tween 80-Na„EDTA (1 + 1) and 40% isopropanol in a Wildman trap flask. Each product was spiked at a different level. For rat hairs, recoveries averaged 82.2% from alfalfa, 88.9% from lemon balm, 80.6% from papaya, and 79.6% from spearmint. Recoveries of whole or equivalent insects from these products averaged 66.1, 218.8, 69.4, and 85.4%, respectively; recoveries of insect fragments from these products averaged 89.6, 94.4, 94.1, and 88.1%, respectively. The method has been adopted official first action for extraction of light filth from whole leaves of alfalfa, papaya, and spearmint. The extension of the method to lemon balm was not recommended because of interferences by intrinsic whole insects, which were the same species as the spike material.

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Extraction of Light Filth from Bean Paste: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/77.5.1143 ◽

1994 ◽

Vol 77 (5) ◽

pp. 1143-1146

Author(s):

John R Bryce ◽

Larry E Glaze

Keyword(s):

United States ◽

Extraction Method ◽

Tap Water ◽

Collaborative Study ◽

The United States ◽

Mineral Oil ◽

New Method ◽

Test Portion ◽

Soybean Paste ◽

Magnetic Stirring

Abstract Bean paste is a popular Asian food frequently imported to the United States. The main varieties are: hot bean and blackbean, which are used in pastry fillings; and soybean paste, which is usually used as a condiment. A new method was developed for the extraction of light filth from bean pastes containing beans and flour, and from hot bean paste containing red pepper. A100 g test portion is boiled in tap water containing Igepal DM-710 and CO-730 and washed with hot tap water on a No. 230 sieve. The residue is transferred to a beaker and boiled in isopropanol in a reflux apparatus. The mixture is transferred to a No. 230 sieve. The residue is washed again, transferred to a 2 L trap flask with 40% isopropanol, boiled with magnetic stirring, cooled, and trapped off with flotation liquid [mineral oil-heptane (85 + 15, v/v)]. Ten laboratories participated in a collaborative study validating the extraction method for the detection of light filth. Average recoveries were 94.9 and 82.8% for insect fragments and rat hairs, respectively. The method has been adopted first action by AOAC INTERNATIONAL.

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Collaborative Study of a New Method for the Extraction of Light Filth from Soy Flour

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/55.1.60 ◽

1972 ◽

Vol 55 (1) ◽

pp. 60-61

Author(s):

Russell G Dent ◽

Michael Brunson

Keyword(s):

Petroleum Ether ◽

Collaborative Study ◽

Enzymatic Digestion ◽

Mineral Oil ◽

New Method ◽

Soy Flour ◽

Wet Sieving ◽

Official Procedure

Abstract Collaborative results are presented for a proposed method for light filth extraction from soy flour. The method involves dispersion in saturated NaCl and isopropanol, wet sieving, and extraction with hot mineral oil from alcohol in a percolator. Average per cent recoveries of spiked elytral fragments and rodent hairs were 88.2 and 65.5, respectively, by the proposed method compared to 64.5 and 56, respectively, by official procedure 40.053. The existing procedure for soy flour is time-consuming because it requires an overnight enzymatic digestion and a hazardous petroleum ether defatting step. The proposed method eliminates both of these steps arid the analysis can be completed in 1–2 hr. It is recommended that the method be adopted as official first action to replace 40.053.

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Collaborative Study of a New Method for the Extraction of Light Filth from White Flour

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/55.3.514 ◽

1972 ◽

Vol 55 (3) ◽

pp. 514-515

Author(s):

Mary T Miller

Keyword(s):

Acid Hydrolysis ◽

Collaborative Study ◽

Mineral Oil ◽

New Method ◽

White Flour

Abstract A new method for the separation of light filth from white flour is based on an autoclave-acid hydrolysis, followed by separation of light fdth elements with mineral oil in a Corning or Kilborn separator. This method is rapid and gives clean papers and good recoveries. The method has been adopted as official first action, to replace 40.045 and 40.046 for extraction of light filth from white flour.

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Extraction of Light Filth from Whole Peppermint Leaves: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/68.4.697 ◽

1985 ◽

Vol 68 (4) ◽

pp. 697-698

Author(s):

Jack Boese ◽

Marvin Nakashima ◽

Larry E Glaze

Keyword(s):

Collaborative Study ◽

Tween 80 ◽

Mineral Oil ◽

Isopropanol Solution ◽

Wet Sieving

Abstract Results are reported for a collaborative study of a method for the extraction of light filth from whole peppermint leaves. A 5 g sample is defatted with isopropanol in a simple reflux appartus. Rat hairs, insect fragments, and whole insects are isolated by wet sieving on a No. 230 sieve, a deaerating boil in 40% isopropanol solution, flotation with Tween 80-Na4edta (1 + 1) and mineral oil-heptane (85 + 15), and trappings in a Wildman trap flask. Average recoveries obtained by 6 collaborators for 3 spike levels of rat hairs (5,10,15) were 83.3, 87.5, and 82.2%, respectively. For whole insects (5,10,15) recoveries averaged 85.0, 80.0 and 77.2% respectively; for insect fragments (20, 30, 50) recoveries averaged 79.6, 88.3, and 84.8%, respectively. The average recoveries for the 3 levels of each analyte were not significantly different. The method has been adopted official first action.

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Collaborative Study of a New Method for Extraction of Light Filth from Reconditioned (Coarsely Ground) Nutmeg

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/54.3.575 ◽

1971 ◽

Vol 54 (3) ◽

pp. 575-578

Author(s):

Alberto W Vazquez

Keyword(s):

Collaborative Study ◽

Mineral Oil ◽

New Method ◽

Official Method ◽

Collaborative Testing

Abstract A new method has been developed for extraction of light filth from coarsely ground nutmeg. Nutmeg tissue is defatted by 3 successive 10 min extractions with boiling CHCI3, after which the solvent is removed by aspiration and an isopropanol rinse. Following sieving on a No. 230 sieve and a brief deaeration and hydrolysis step in acidified boiling 60% ethanol-CaCl2, light filth elements are extracted with mineral oil from the 60% ethanol-CaCl2 mixture. Collaborative testing of the proposed method yielded satisfactory recoveries of added light filth elements. The official method for ground spices, 40.106(b), was compared in a collaborative study with the proposed method for the analysis of ground nutmeg and mace. Results were encouraging, but further study is indicated. The method as described for reconditioned nutmeg has been adopted as official first action.

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