4,4′-(Diazoamino)-bis(5-methoxy-2-methylbenzenesulfonic Acid): Preparation and Determination in FD&C Red No. 40

1976 ◽  
Vol 59 (1) ◽  
pp. 5-11
Author(s):  
John E Bailey ◽  
Elizabeth A Cox
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Pressure Liquid Chromatography ◽  
Sulfonic Acid ◽  
Sodium Acetate ◽  
Diazonium Salt

Abstract 4,4′-(Diazoamino)-bis(5-methoxy-2-methylbenzenesulfonic acid) (CSAT) has been found in samples of FD&C Red No. 40 submitted for certification, using high-pressure liquid chromatography (HPLC). CSAT is synthesized by diazotizing cresidine sulfonic acid (CSA) and coupling the diazonium salt with undiazotized CSA in the presence of sodium acetate. A method for quantitative determination of CSAT in FD&C Red No. 40, using HPLC and an untested method of correcting for interfering compounds, is presented.

Rapid colorimetric assay for acetaminophen without salicylate or phenylephrine interference.

1982 ◽  
Vol 28 (5) ◽  
pp. 1171-1173 ◽  
Author(s):  
M B Swanson ◽  
M I Walters
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Pressure Liquid Chromatography ◽  
Sulfonic Acid ◽  
Colorimetric Assay ◽  
Colored Complex

Abstract A simple rapid, and economical procedure is presented for the quantitative determination of acetaminophen in serum. Acetaminophen is extracted into solvent, and, when back-extracted into base, is simultaneously reacted with Folin-Ciocalteau reagent to form a colored complex. The potential interference of bilirubin is eliminated by treatment with p-diazobenzene sulfonic acid. Results obtained with this method agree with those obtained by "high-pressure" liquid chromatography.

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