Determination of Inorganic Tin in Biological Samples by Hydride Generation-Atomic Absorption Spectrometry after Silica Gel Cleanup

Abstract A method is described for the determination of inorganic tin in biological samples by hydride generation-atomic absorption spectrometry (HG-AAS). A sample is extracted with ethyl acetate after addition of HC1 and NaCl. The concentrated extract is passed through a silica gel column. The column is washed with ethanol, water, and 0.2N HC1 successively, and then inorganic tin is eluted with 2N HC1 and measured by HG-AAS. Recoveries from fish muscle spiked with 0.1 ng/g Sn4+ are 78.9 ± 4.2% (average ± standard deviation, n = 5). The detection limit is 0.01 jug/g as Sn.

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Preconcentration and Determination of Tellurium in Garlic Samples by Hydride Generation Atomic Absorption Spectrometry

Journal of AOAC International ◽

10.1093/jaoac/88.4.1242 ◽

2005 ◽

Vol 88 (4) ◽

pp. 1242-1246 ◽

Cited By ~ 7

Author(s):

Marcos M Kaplan ◽

Soledad Cerutti ◽

José A Salonia ◽

José A Gásquez ◽

Luis D Martinez

Keyword(s):

Standard Deviation ◽

Atomic Absorption Spectrometry ◽

Atomic Absorption ◽

Hydride Generation ◽

Absorption Spectrometry ◽

Calibration Graph ◽

Lanthanum Hydroxide ◽

Relative Standard ◽

Hydroxide Precipitate

Abstract A procedure for the determination of traces of total tellurium (Te) in garlic (Allium sativa) is described that combines hydride generation atomic absorption spectrometry with preconcentration of the analyte by coprecipitation. The samples, each spiked with lanthanum nitrate (20 mg/L), are introduced into an Amberlite XAD-4 resin and mixed with ammonium buffer (pH 9.1). Te is preconcentrated by coprecipitation with the generated lanthanum hydroxide precipitate. The precipitate is quantitatively collected in the resin, eluted with hydrochloric acid, and then transferred into the atomizer device. Considering a sample consumption of 25 mL, an enrichment factor of 10 was obtained. The detection limit (3σ) was 0.03 μg/L, and the precision (relative standard deviation) was 3.5% (n = 10) at the 10 μg/L level. The calibration graph using the preconcentration system for Te was linear with a correlation coefficient of 0.9993. Satisfactory results were obtained for the analysis of Te in garlic samples.

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Determination of Inorganic Arsenic in Marine Food Samples by Hydrochloric Acid Distillation and Flow-Injection Hydride-Generation Atomic Absorption Spectrometry

Journal of AOAC International ◽

10.1093/jaoac/82.5.1217 ◽

1999 ◽

Vol 82 (5) ◽

pp. 1217-1223 ◽

Cited By ~ 7

Author(s):

Joar Karsten Øygard ◽

Anne-Katrine Lundebye ◽

Kåre Julshamn

Keyword(s):

Atomic Absorption Spectrometry ◽

Atomic Absorption ◽

Flow Injection ◽

Biological Samples ◽

Certified Reference Materials ◽

Hydride Generation ◽

Absorption Spectrometry ◽

Fish Fillet ◽

Inorganic As

Abstract A simple, rapid, and reliable method was developed for determination of inorganic As in biological samples such as fish fillet. Inorganic AS was distilled from the sample as AsCl3 with HCI. The separated inorganic AS was determined by flow-injection hydride-generation atomic absorption spectrometry after prereduction with Kl and HCI. The influences of various concentrations of Kl, ascorbic acid, and HCI in the prereduction stage; NaBH4 as the reductant; and HCI as the carrier solution on analytical results were studied. Digestion was performed in a Kjeldahl digestion system for 75 min with 4 mL nitric acid and 1 mL sulfuric acid at 380°C. The concentrations of inorganic As in samples were less than 0.1 mg/kg dry weight for fish fillet and somewhat higher for crustaceans and bivalve molluscs. The total and inorganic As contents of various marine biological samples and certified reference materials were determined.

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