scholarly journals Reversed-Phase Thin-Layer Chromatography with Different Stationary Phases in Studies of Quantitative Structure–Biological Activity Relationship of New Antimycotic Compounds

1999 ◽  
Vol 82 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Jan K Rózylo ◽  
Anna Zabiñska ◽  
Joanna Matysiak ◽  
Andrzej Niewiadomy
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Quantitative Structure Activity Relationship ◽  
Reversed Phase ◽  
Activity Relationship ◽  
Quantitative Structure ◽  
Acetone Concentration ◽  
Qsar Studies ◽  
Layer Chromatography

Abstract Reversed-phase thin-layer chromatography with RP-8, RP-18, and RP-18W stationary phases was used in quantitative structure–activity relationship (QSAR) studies of new antimycotic compounds. The retention behavior of 10 dihydroxythioben-zanilides was examined for acquisition of log k data. With water–acetone mixtures as the mobile phases, the concentration range for which the correlation between log k′ and acetone concentration is linear was established for each stationary phase and used to determine hydrophobicity parameters log k′w by linear extrapolation. The effect of substituents on retention constants was quantitated by using the group contribution parameters τw. On the basis of QSAR equations obtained from these studies, log k′w, data can be used to predict antifungal activities of dihydroxythiobenzanilides with satisfactory accuracy.

scholarly journals Reversed-phase thin-layer chromatography behavior of aldopentose derivatives

2012 ◽  
Vol 66 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Dragana Livaja-Popovic ◽  
Eva Loncar ◽  
Lidija Jevric ◽  
Radomir Malbasa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chromatographic Behavior ◽  
Log P ◽  
Retention Factors ◽  
Mobile Phases ◽  
Acetone Water ◽  
Layer Chromatography

Quantitative structure-retention relationships (QSRR) have been used to study the chromatographic behavior of some aldopentose. The behavior of aldopentose derivatives was investigated by means of the reversed-phase thin-layer chromatography (RP TLC) on the silica gel impregnated with paraffin oil stationary phases. Binary mixtures of methanol-water, acetone-water and dioxane-water were used as mobile phases. Retention factors, RM0, corresponding to zero percent organic modifier in the aqueous mobile phase was determined. Lipophilicity C0 was calculated as the ratio of the intercept and slope values. There was satisfactory correlation between them and log P values calculated using different theoretical procedures. Some of these correlations offer very good predicting models, which are important for a better understanding of the relationships between chemical structure and retention. The study showed that the hydrophobic parameters RM0 and C0 can be used as a measures of lipophilicity of investigated compounds.

Microchannel Thin Layer Chromatography with in situ Plate Scanning Micro-DRIFTS Detection Using Plain and Polybutadiene Modified Zirconia Stationary Phases

2002 ◽  
Vol 56 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Beata A. Musial ◽  
André J. Sommer ◽  
Neil D. Danielson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Diffuse Reflectance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Diffuse Reflectance Infrared ◽  
Layer Chromatography ◽  
Tlc Separation ◽  
Zirconia Stationary Phases

Thin layer chromatography (TLC) of various dyes is compared in microchannels packed with either bare zirconia (normal phase) or polybutadiene (PBD) modified zirconia (reversed phase). In situ micro-diffuse reflectance infrared Fourier transform spectroscopy (μDRIFTS) of the analyte spots is possible due to the low absorption background of both plain and PBD zirconia. An instrument coupling μDRIFTS with a motorized stage that could profile the microchannel TLC plate is developed. The retention order of anthracene and pyrene probes as a function of percent acetonitrile are generally as expected on PBD zirconia but opposite for plain zirconia. Impurities in technical grade methylene blue can be determined after TLC separation on plain zirconia. The separation and infrared identification of various dyes such as dichlorofluorescein and rhodamine B on PBD zirconia is improved using a mobile phase with an additive such as phosphate or dihexylamine that prevents band streaking.

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